Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation
Isolation and studies of novel, crude oil biodegrading thermophilic strains may provide a wider knowledge in understanding their role in petroleum degradation. In this study, the screening of ten new thermophilic strains revealed that all strains were alkane hydroxylase producers and seven of them p...
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MDPI
2020
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| Online Access: | http://eprints.sunway.edu.my/1367/ http://eprints.sunway.edu.my/1367/1/Malihe%20Newly%20isolated%20alkane.pdf |
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| author | Durratul, F. Y. Raja Noor Zaliha, R. A. R. Masomian, Malihe * Mohd Shukri, M. A. Leow, Thean Chor |
| author_facet | Durratul, F. Y. Raja Noor Zaliha, R. A. R. Masomian, Malihe * Mohd Shukri, M. A. Leow, Thean Chor |
| author_sort | Durratul, F. Y. |
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| description | Isolation and studies of novel, crude oil biodegrading thermophilic strains may provide a wider knowledge in understanding their role in petroleum degradation. In this study, the screening of ten new thermophilic strains revealed that all strains were alkane hydroxylase producers and seven of them produced lipase concurrently. Three best strains were characterized and identified through 16S rRNA sequence analysis as Geobacillus sp. D4, Geobacillus sp. D7, and Anoxybacillus geothermalis D9 with GenBank accession numbers MK615934.1, MK615935.1, and MK615936.1, respectively. Gas chromatography (GC) analysis showed that all three strains were able to breakdown various compounds in crude oil such as alkanes, toxic poly-aromatic hydrocarbons (PAHs), organosulfur, carboxylic acids, alkene, resins, organosilicon, alcohol, organochlorine, and ester. For the first time, alkane hydroxylase and lipase activity as well as crude oil degradation by A. geothermalis species were reported. Geobacillus sp. D7 is the best alkane degrader followed by A. geothermalis D9 and Geobacillus sp. D4 with 17.3%, 13.1%, and 12.1% biodegradation efficiency (BE%), respectively. The potential of thermophiles isolated can be explored further for bioremediation of sites polluted by petroleum and oil spills. |
| first_indexed | 2025-11-14T21:17:01Z |
| format | Article |
| id | sunway-1367 |
| institution | Sunway University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:17:01Z |
| publishDate | 2020 |
| publisher | MDPI |
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| spelling | sunway-13672020-09-21T06:25:16Z http://eprints.sunway.edu.my/1367/ Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation Durratul, F. Y. Raja Noor Zaliha, R. A. R. Masomian, Malihe * Mohd Shukri, M. A. Leow, Thean Chor QR355 Virology Isolation and studies of novel, crude oil biodegrading thermophilic strains may provide a wider knowledge in understanding their role in petroleum degradation. In this study, the screening of ten new thermophilic strains revealed that all strains were alkane hydroxylase producers and seven of them produced lipase concurrently. Three best strains were characterized and identified through 16S rRNA sequence analysis as Geobacillus sp. D4, Geobacillus sp. D7, and Anoxybacillus geothermalis D9 with GenBank accession numbers MK615934.1, MK615935.1, and MK615936.1, respectively. Gas chromatography (GC) analysis showed that all three strains were able to breakdown various compounds in crude oil such as alkanes, toxic poly-aromatic hydrocarbons (PAHs), organosulfur, carboxylic acids, alkene, resins, organosilicon, alcohol, organochlorine, and ester. For the first time, alkane hydroxylase and lipase activity as well as crude oil degradation by A. geothermalis species were reported. Geobacillus sp. D7 is the best alkane degrader followed by A. geothermalis D9 and Geobacillus sp. D4 with 17.3%, 13.1%, and 12.1% biodegradation efficiency (BE%), respectively. The potential of thermophiles isolated can be explored further for bioremediation of sites polluted by petroleum and oil spills. MDPI 2020-08-01 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/1367/1/Malihe%20Newly%20isolated%20alkane.pdf Durratul, F. Y. and Raja Noor Zaliha, R. A. R. and Masomian, Malihe * and Mohd Shukri, M. A. and Leow, Thean Chor (2020) Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation. Catalysts, 10 (8). p. 851. ISSN 2073-4344 http://doi.org/10.3390/catal10080851 doi:10.3390/catal10080851 |
| spellingShingle | QR355 Virology Durratul, F. Y. Raja Noor Zaliha, R. A. R. Masomian, Malihe * Mohd Shukri, M. A. Leow, Thean Chor Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation |
| title | Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation |
| title_full | Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation |
| title_fullStr | Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation |
| title_full_unstemmed | Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation |
| title_short | Newly isolated alkane hydroxylase and lipase producing Geobacillus and Anoxybacillus species involved in crude oil degradation |
| title_sort | newly isolated alkane hydroxylase and lipase producing geobacillus and anoxybacillus species involved in crude oil degradation |
| topic | QR355 Virology |
| url | http://eprints.sunway.edu.my/1367/ http://eprints.sunway.edu.my/1367/ http://eprints.sunway.edu.my/1367/ http://eprints.sunway.edu.my/1367/1/Malihe%20Newly%20isolated%20alkane.pdf |