Evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using Punica Granatum fruit peel extract

Magnetic iron oxide nanoparticles (IONPs) are increasingly being studied for potential anticancer application to circumvent off-target cytotoxicity and other side effects from conventional chemotherapy. In this study, four different weight% of Punica granatum fruit peel extract were used as green st...

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Main Authors: Yusefi, Mostafa, Shameli, Kamyar, Roshafima, R. A., Pang, Siew Wai *, Teow, Sin Yeang *
Format: Article
Published: Elsevier 2020
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Online Access:http://eprints.sunway.edu.my/1196/
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author Yusefi, Mostafa
Shameli, Kamyar
Roshafima, R. A.
Pang, Siew Wai *
Teow, Sin Yeang *
author_facet Yusefi, Mostafa
Shameli, Kamyar
Roshafima, R. A.
Pang, Siew Wai *
Teow, Sin Yeang *
author_sort Yusefi, Mostafa
building SU Institutional Repository
collection Online Access
description Magnetic iron oxide nanoparticles (IONPs) are increasingly being studied for potential anticancer application to circumvent off-target cytotoxicity and other side effects from conventional chemotherapy. In this study, four different weight% of Punica granatum fruit peel extract were used as green stabilizers to synthesize IONPs followed by a series of characterization experiments and in vitro anticancer evaluation. Results revealed that all synthesized IONPs have high degree of crystallinity and purity. The optimum saturation magnetization, and hydrodynamic size were found to be ∼69 emu.g−1, and 26.52 nm, respectively. Morphological studies demonstrated that adding peel extract decreased size of NPs with the average particle size below 11 nm. Cytotoxicity assay showed that the IONPs were not reactive (IC50 > 250 μg/ml) against colon (HCT116), breast (MCF7), cervical (HeLa) and lung (A549) cancer cell lines and two normal cell lines derived from human colon and kidney (CCD112 and HEK293). Specifically, IONPs with 2 and 4 wt% of peel extract displayed potent anticancer activities (IC50 of 197.46 and 85.06 μg/ml respectively) against nasopharyngeal carcinoma (NPC) cell line, HONE1. Overall, this study shows the characterization of green synthesized IONPs and its potential use as anticancer therapeutic agents against NPC cells
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spelling sunway-11962019-12-31T01:27:13Z http://eprints.sunway.edu.my/1196/ Evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using Punica Granatum fruit peel extract Yusefi, Mostafa Shameli, Kamyar Roshafima, R. A. Pang, Siew Wai * Teow, Sin Yeang * RM Therapeutics. Pharmacology Magnetic iron oxide nanoparticles (IONPs) are increasingly being studied for potential anticancer application to circumvent off-target cytotoxicity and other side effects from conventional chemotherapy. In this study, four different weight% of Punica granatum fruit peel extract were used as green stabilizers to synthesize IONPs followed by a series of characterization experiments and in vitro anticancer evaluation. Results revealed that all synthesized IONPs have high degree of crystallinity and purity. The optimum saturation magnetization, and hydrodynamic size were found to be ∼69 emu.g−1, and 26.52 nm, respectively. Morphological studies demonstrated that adding peel extract decreased size of NPs with the average particle size below 11 nm. Cytotoxicity assay showed that the IONPs were not reactive (IC50 > 250 μg/ml) against colon (HCT116), breast (MCF7), cervical (HeLa) and lung (A549) cancer cell lines and two normal cell lines derived from human colon and kidney (CCD112 and HEK293). Specifically, IONPs with 2 and 4 wt% of peel extract displayed potent anticancer activities (IC50 of 197.46 and 85.06 μg/ml respectively) against nasopharyngeal carcinoma (NPC) cell line, HONE1. Overall, this study shows the characterization of green synthesized IONPs and its potential use as anticancer therapeutic agents against NPC cells Elsevier 2020-12-10 Article PeerReviewed Yusefi, Mostafa and Shameli, Kamyar and Roshafima, R. A. and Pang, Siew Wai * and Teow, Sin Yeang * (2020) Evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using Punica Granatum fruit peel extract. Journal of Molecular Structure, 1204. p. 127539. ISSN 0022 2860 http://doi.org/10.1016/j.molstruc.2019.127539 doi:10.1016/j.molstruc.2019.127539
spellingShingle RM Therapeutics. Pharmacology
Yusefi, Mostafa
Shameli, Kamyar
Roshafima, R. A.
Pang, Siew Wai *
Teow, Sin Yeang *
Evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using Punica Granatum fruit peel extract
title Evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using Punica Granatum fruit peel extract
title_full Evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using Punica Granatum fruit peel extract
title_fullStr Evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using Punica Granatum fruit peel extract
title_full_unstemmed Evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using Punica Granatum fruit peel extract
title_short Evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using Punica Granatum fruit peel extract
title_sort evaluating anticancer activity of plant-mediated synthesized iron oxide nanoparticles using punica granatum fruit peel extract
topic RM Therapeutics. Pharmacology
url http://eprints.sunway.edu.my/1196/
http://eprints.sunway.edu.my/1196/
http://eprints.sunway.edu.my/1196/