Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system
Background: In vitro modelling of cancer cells is becoming more complex due to prevailing evidence of intimate interactions between cancer cells and their surrounding stroma. A co-culture system which consists of more than one cell type is physiologically more relevant and thus, could serve as a use...
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| Format: | Article |
| Language: | English |
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BMC (Springer Nature)
2019
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| Online Access: | http://eprints.sunway.edu.my/1152/ http://eprints.sunway.edu.my/1152/1/Teow%20Sin%20Yeang%20Development%20of%20a%20luciferaseluciferin%20cell.pdf |
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| author | Teow, Sin Yeang * Liew, Kitson Che Mat, M. Marini, M. Norazlin, A. A. Chu, Tai Lin Munirah, A. Khoo, Alan Soo Beng |
| author_facet | Teow, Sin Yeang * Liew, Kitson Che Mat, M. Marini, M. Norazlin, A. A. Chu, Tai Lin Munirah, A. Khoo, Alan Soo Beng |
| author_sort | Teow, Sin Yeang * |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Background: In vitro modelling of cancer cells is becoming more complex due to prevailing evidence of intimate interactions between cancer cells and their surrounding stroma. A co-culture system which consists of more than one cell type is physiologically more relevant and thus, could serve as a useful model for various biological studies. An assay that specifically detects the phenotypic changes of cancer cells in a multi-cellular system is lacking for nasopharyngeal carcinoma (NPC).
Results: Here, we describe a luciferase/luciferin (XenoLuc) assay that could specifically measure changes in the proliferation of cancer cells in the co-culture system using two modified NPC patient-derived tumour xenograft (PDTXs) cells: Xeno284-gfp-luc2 and XenoB110-gfp-luc2. Through this assay, we are able to show that the growth of NPC xenograft cells in both two-dimensional (2D) and three-dimensional (3D) models was enhanced when co-cultured with normal human dermal fibroblasts (NHDFs). In addition, potential applications of this assay in in vitro drug or inhibitor screening experiments are also illustrated.
Conclusions: XenoLuc assay is specific, sensitive, rapid and cost-effective for measuring the growth of luciferase-expressing cells in a co- or multiple-culture system. This assay may also be adapted for tumour microenvironment studies as well as drug screening experiments in more complex 3D co-culture systems. |
| first_indexed | 2025-11-14T21:16:12Z |
| format | Article |
| id | sunway-1152 |
| institution | Sunway University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:16:12Z |
| publishDate | 2019 |
| publisher | BMC (Springer Nature) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | sunway-11522019-11-28T03:59:25Z http://eprints.sunway.edu.my/1152/ Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system Teow, Sin Yeang * Liew, Kitson Che Mat, M. Marini, M. Norazlin, A. A. Chu, Tai Lin Munirah, A. Khoo, Alan Soo Beng QH301 Biology R Medicine (General) Background: In vitro modelling of cancer cells is becoming more complex due to prevailing evidence of intimate interactions between cancer cells and their surrounding stroma. A co-culture system which consists of more than one cell type is physiologically more relevant and thus, could serve as a useful model for various biological studies. An assay that specifically detects the phenotypic changes of cancer cells in a multi-cellular system is lacking for nasopharyngeal carcinoma (NPC). Results: Here, we describe a luciferase/luciferin (XenoLuc) assay that could specifically measure changes in the proliferation of cancer cells in the co-culture system using two modified NPC patient-derived tumour xenograft (PDTXs) cells: Xeno284-gfp-luc2 and XenoB110-gfp-luc2. Through this assay, we are able to show that the growth of NPC xenograft cells in both two-dimensional (2D) and three-dimensional (3D) models was enhanced when co-cultured with normal human dermal fibroblasts (NHDFs). In addition, potential applications of this assay in in vitro drug or inhibitor screening experiments are also illustrated. Conclusions: XenoLuc assay is specific, sensitive, rapid and cost-effective for measuring the growth of luciferase-expressing cells in a co- or multiple-culture system. This assay may also be adapted for tumour microenvironment studies as well as drug screening experiments in more complex 3D co-culture systems. BMC (Springer Nature) 2019-06-14 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/1152/1/Teow%20Sin%20Yeang%20Development%20of%20a%20luciferaseluciferin%20cell.pdf Teow, Sin Yeang * and Liew, Kitson and Che Mat, M. and Marini, M. and Norazlin, A. A. and Chu, Tai Lin and Munirah, A. and Khoo, Alan Soo Beng (2019) Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system. BMC Biotechnology, 19 (34). ISSN 1472-6750 http://doi.org/10.1186/s12896-019-0528-4 doi:10.1186/s12896-019-0528-4 |
| spellingShingle | QH301 Biology R Medicine (General) Teow, Sin Yeang * Liew, Kitson Che Mat, M. Marini, M. Norazlin, A. A. Chu, Tai Lin Munirah, A. Khoo, Alan Soo Beng Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system |
| title | Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system |
| title_full | Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system |
| title_fullStr | Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system |
| title_full_unstemmed | Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system |
| title_short | Development of a luciferase/luciferin cell proliferation (XenoLuc) assay for real-time measurements of Gfp-Luc2-modified cells in a co-culture system |
| title_sort | development of a luciferase/luciferin cell proliferation (xenoluc) assay for real-time measurements of gfp-luc2-modified cells in a co-culture system |
| topic | QH301 Biology R Medicine (General) |
| url | http://eprints.sunway.edu.my/1152/ http://eprints.sunway.edu.my/1152/ http://eprints.sunway.edu.my/1152/ http://eprints.sunway.edu.my/1152/1/Teow%20Sin%20Yeang%20Development%20of%20a%20luciferaseluciferin%20cell.pdf |