Potential anticancer activity of protocatechuic acid loaded in montmorillonite/Fe3O4 nanocomposites stabilized by seaweed Kappaphycus alvarezii
Superparamagnetic magnetite nanocomposites (Fe3O4-NCs) were successfully synthesized, which comprised of montmorillonite (MMT) as matrix support, Kappaphycus alvarezii (SW) as bio-stabilizer and Fe3O4 as filler in the composites to form MMT/SW/Fe3O4-NCs. Nanocomposite with 0.5 g Fe3O4 (MMT/SW/0.5Fe3...
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| Format: | Article |
| Language: | English |
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Elsevier
2019
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| Online Access: | http://eprints.sunway.edu.my/1144/ http://eprints.sunway.edu.my/1144/1/Teow%20Sin%20Yeang%20potential.pdf |
| _version_ | 1848801986333376512 |
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| author | Yew, Yen Pin Shameli, Kamyar Shaza Eva M, Yuki, Nagao Teow, Sin Yeang * Lee, Kar Xin Eleen Dayana, M. I. |
| author_facet | Yew, Yen Pin Shameli, Kamyar Shaza Eva M, Yuki, Nagao Teow, Sin Yeang * Lee, Kar Xin Eleen Dayana, M. I. |
| author_sort | Yew, Yen Pin |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Superparamagnetic magnetite nanocomposites (Fe3O4-NCs) were successfully synthesized, which comprised of montmorillonite (MMT) as matrix support, Kappaphycus alvarezii (SW) as bio-stabilizer and Fe3O4 as filler in the composites to form MMT/SW/Fe3O4-NCs. Nanocomposite with 0.5 g Fe3O4 (MMT/SW/0.5Fe3O4) was selected for anticancer activity study because it revealed high crystallinity, particle size of 7.2 ± 1.7 nm with majority of spherical shape, and Ms = 5.85 emu/g with negligible coercivity. Drug loading and release studies were carried out using protocatechuic acid (PCA) as the model for anticancer drug, which showed 19% and 87% of PCA release in pH 7.4 and 4.8, respectively. Monolayer anticancer assay showed that PCA-loaded MMT/SW/Fe3O4 (MMT/SW/Fe3O4-PCA) had selectivity towards HCT116 (colorectal cancer cell line). Although MMT/SW/Fe3O4-PCA (0.64 mg/mL) showed higher IC50 than PCA (0.148 mg/mL) and MMT/SW/Fe3O4 (0.306 mg/mL, MMT/SW/Fe3O4-PCA showed more effective killing towards tumour spheroid model generated from HCT116. The IC50 for MMT/SW/Fe3O4-PCA, MMT/SW/Fe3O4 and PCA were 0.132, 0.23 and 0.55 mg/mL, respectively. This suggests the improved penetration efficiency and drug release of MMT/SW/Fe3O4-PCA towards HCT116 spheroids. Moreover, concentration that lower than 2 mg/mL MMT/SW/Fe3O4-PCA did not result any hemolysis in human blood, which suggests them to be ideal for intravenous injection. This study highlights the potential of MMT/SW/Fe3O4-NCs as drug delivery agent |
| first_indexed | 2025-11-14T21:16:10Z |
| format | Article |
| id | sunway-1144 |
| institution | Sunway University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:16:10Z |
| publishDate | 2019 |
| publisher | Elsevier |
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| repository_type | Digital Repository |
| spelling | sunway-11442019-12-03T09:44:18Z http://eprints.sunway.edu.my/1144/ Potential anticancer activity of protocatechuic acid loaded in montmorillonite/Fe3O4 nanocomposites stabilized by seaweed Kappaphycus alvarezii Yew, Yen Pin Shameli, Kamyar Shaza Eva M, Yuki, Nagao Teow, Sin Yeang * Lee, Kar Xin Eleen Dayana, M. I. RC0254 Neoplasms. Tumors. Oncology (including Cancer) Superparamagnetic magnetite nanocomposites (Fe3O4-NCs) were successfully synthesized, which comprised of montmorillonite (MMT) as matrix support, Kappaphycus alvarezii (SW) as bio-stabilizer and Fe3O4 as filler in the composites to form MMT/SW/Fe3O4-NCs. Nanocomposite with 0.5 g Fe3O4 (MMT/SW/0.5Fe3O4) was selected for anticancer activity study because it revealed high crystallinity, particle size of 7.2 ± 1.7 nm with majority of spherical shape, and Ms = 5.85 emu/g with negligible coercivity. Drug loading and release studies were carried out using protocatechuic acid (PCA) as the model for anticancer drug, which showed 19% and 87% of PCA release in pH 7.4 and 4.8, respectively. Monolayer anticancer assay showed that PCA-loaded MMT/SW/Fe3O4 (MMT/SW/Fe3O4-PCA) had selectivity towards HCT116 (colorectal cancer cell line). Although MMT/SW/Fe3O4-PCA (0.64 mg/mL) showed higher IC50 than PCA (0.148 mg/mL) and MMT/SW/Fe3O4 (0.306 mg/mL, MMT/SW/Fe3O4-PCA showed more effective killing towards tumour spheroid model generated from HCT116. The IC50 for MMT/SW/Fe3O4-PCA, MMT/SW/Fe3O4 and PCA were 0.132, 0.23 and 0.55 mg/mL, respectively. This suggests the improved penetration efficiency and drug release of MMT/SW/Fe3O4-PCA towards HCT116 spheroids. Moreover, concentration that lower than 2 mg/mL MMT/SW/Fe3O4-PCA did not result any hemolysis in human blood, which suggests them to be ideal for intravenous injection. This study highlights the potential of MMT/SW/Fe3O4-NCs as drug delivery agent Elsevier 2019-11-06 Article PeerReviewed text en cc_by_nc_nd_4 http://eprints.sunway.edu.my/1144/1/Teow%20Sin%20Yeang%20potential.pdf Yew, Yen Pin and Shameli, Kamyar and Shaza Eva M, and Yuki, Nagao and Teow, Sin Yeang * and Lee, Kar Xin and Eleen Dayana, M. I. (2019) Potential anticancer activity of protocatechuic acid loaded in montmorillonite/Fe3O4 nanocomposites stabilized by seaweed Kappaphycus alvarezii. International Journal of Pharmaceutics. p. 118743. ISSN 03785173 http://doi.org/10.1016/j.ijpharm.2019.118743 doi:10.1016/j.ijpharm.2019.118743 |
| spellingShingle | RC0254 Neoplasms. Tumors. Oncology (including Cancer) Yew, Yen Pin Shameli, Kamyar Shaza Eva M, Yuki, Nagao Teow, Sin Yeang * Lee, Kar Xin Eleen Dayana, M. I. Potential anticancer activity of protocatechuic acid loaded in montmorillonite/Fe3O4 nanocomposites stabilized by seaweed Kappaphycus alvarezii |
| title | Potential anticancer activity of protocatechuic acid loaded in montmorillonite/Fe3O4 nanocomposites stabilized by seaweed Kappaphycus alvarezii |
| title_full | Potential anticancer activity of protocatechuic acid loaded in montmorillonite/Fe3O4 nanocomposites stabilized by seaweed Kappaphycus alvarezii |
| title_fullStr | Potential anticancer activity of protocatechuic acid loaded in montmorillonite/Fe3O4 nanocomposites stabilized by seaweed Kappaphycus alvarezii |
| title_full_unstemmed | Potential anticancer activity of protocatechuic acid loaded in montmorillonite/Fe3O4 nanocomposites stabilized by seaweed Kappaphycus alvarezii |
| title_short | Potential anticancer activity of protocatechuic acid loaded in montmorillonite/Fe3O4 nanocomposites stabilized by seaweed Kappaphycus alvarezii |
| title_sort | potential anticancer activity of protocatechuic acid loaded in montmorillonite/fe3o4 nanocomposites stabilized by seaweed kappaphycus alvarezii |
| topic | RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.sunway.edu.my/1144/ http://eprints.sunway.edu.my/1144/ http://eprints.sunway.edu.my/1144/ http://eprints.sunway.edu.my/1144/1/Teow%20Sin%20Yeang%20potential.pdf |