Delineating the plausible molecular vaccine candidates and drug targets of multidrug-resistant Acinetobacter baumannii
Nosocomial infections have become alarming with the increase of multidrug-resistant bacterial strains of Acinetobacter baumannii. Being the causative agent in ∼80% of the cases, these pathogenic gram-negative species could be deadly for hospitalized patients, especially in intensive care units utili...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media
2019
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| Online Access: | http://eprints.sunway.edu.my/1117/ http://eprints.sunway.edu.my/1117/1/Lahiri%20Delineating%20the%20Plausible.pdf |
| _version_ | 1848801978691354624 |
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| author | Mujawar, Shama * Mishra, Rohit Pawar, Shrikant Gatherer, Derek Lahiri, Chandrajit * |
| author_facet | Mujawar, Shama * Mishra, Rohit Pawar, Shrikant Gatherer, Derek Lahiri, Chandrajit * |
| author_sort | Mujawar, Shama * |
| building | SU Institutional Repository |
| collection | Online Access |
| description | Nosocomial infections have become alarming with the increase of multidrug-resistant bacterial strains of Acinetobacter baumannii. Being the causative agent in ∼80% of the cases, these pathogenic gram-negative species could be deadly for hospitalized patients, especially in intensive care units utilizing ventilators, urinary catheters, and nasogastric tubes. Primarily infecting an immuno-compromised system, they are resistant to most antibiotics and are the root cause of various types of
opportunistic infections including but not limited to septicemia, endocarditis, meningitis, pneumonia, skin, and wound sepsis and even urinary tract infections. Conventional experimental methods including typing, computational methods encompassing comparative genomics, and combined methods of reverse vaccinology and proteomics
had been proposed to differentiate and develop vaccines and/or drugs for several outbreak strains. However, identifying proteins suitable enough to be posed as drug
targets and/or molecular vaccines against the multidrug-resistant pathogenic bacterial strains has probably remained an open issue to address. In these cases of novel protein identification, the targets either are uncharacterized or have been unable to confer the
most coveted protection either in the form of molecular vaccine candidates or as drug targets. Here, we report a strategic approach with the 3,766 proteins from the whole
genome of A. baumannii ATCC19606 (AB) to rationally identify plausible candidates and propose them as future molecular vaccine candidates and/or drug targets. Essentially, we started with mapping the vaccine candidates (VaC) and virulence factors (ViF) of A. baumannii strain AYE onto strain ATCC19606 to identify them in the latter. We move on to build small networks of VaC and ViF to conceptualize their position in the network space
of the whole genomic protein interactome (GPIN) and rationalize their candidature for drugs and/or molecular vaccines. To this end, we propose new sets of known proteins unearthed from interactome built using key factors, KeF, potent enough to compete with VaC and ViF. Our method is the first of its kind to propose, albeit theoretically, a rational approach to identify crucial proteins and pose them for candidates of vaccines and/or
drugs effective enough to combat the deadly pathogenic threats of A. baumannii. |
| first_indexed | 2025-11-14T21:16:03Z |
| format | Article |
| id | sunway-1117 |
| institution | Sunway University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T21:16:03Z |
| publishDate | 2019 |
| publisher | Frontiers Media |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | sunway-11172019-11-12T03:25:27Z http://eprints.sunway.edu.my/1117/ Delineating the plausible molecular vaccine candidates and drug targets of multidrug-resistant Acinetobacter baumannii Mujawar, Shama * Mishra, Rohit Pawar, Shrikant Gatherer, Derek Lahiri, Chandrajit * QH301 Biology QR Microbiology Nosocomial infections have become alarming with the increase of multidrug-resistant bacterial strains of Acinetobacter baumannii. Being the causative agent in ∼80% of the cases, these pathogenic gram-negative species could be deadly for hospitalized patients, especially in intensive care units utilizing ventilators, urinary catheters, and nasogastric tubes. Primarily infecting an immuno-compromised system, they are resistant to most antibiotics and are the root cause of various types of opportunistic infections including but not limited to septicemia, endocarditis, meningitis, pneumonia, skin, and wound sepsis and even urinary tract infections. Conventional experimental methods including typing, computational methods encompassing comparative genomics, and combined methods of reverse vaccinology and proteomics had been proposed to differentiate and develop vaccines and/or drugs for several outbreak strains. However, identifying proteins suitable enough to be posed as drug targets and/or molecular vaccines against the multidrug-resistant pathogenic bacterial strains has probably remained an open issue to address. In these cases of novel protein identification, the targets either are uncharacterized or have been unable to confer the most coveted protection either in the form of molecular vaccine candidates or as drug targets. Here, we report a strategic approach with the 3,766 proteins from the whole genome of A. baumannii ATCC19606 (AB) to rationally identify plausible candidates and propose them as future molecular vaccine candidates and/or drug targets. Essentially, we started with mapping the vaccine candidates (VaC) and virulence factors (ViF) of A. baumannii strain AYE onto strain ATCC19606 to identify them in the latter. We move on to build small networks of VaC and ViF to conceptualize their position in the network space of the whole genomic protein interactome (GPIN) and rationalize their candidature for drugs and/or molecular vaccines. To this end, we propose new sets of known proteins unearthed from interactome built using key factors, KeF, potent enough to compete with VaC and ViF. Our method is the first of its kind to propose, albeit theoretically, a rational approach to identify crucial proteins and pose them for candidates of vaccines and/or drugs effective enough to combat the deadly pathogenic threats of A. baumannii. Frontiers Media 2019-06-20 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/1117/1/Lahiri%20Delineating%20the%20Plausible.pdf Mujawar, Shama * and Mishra, Rohit and Pawar, Shrikant and Gatherer, Derek and Lahiri, Chandrajit * (2019) Delineating the plausible molecular vaccine candidates and drug targets of multidrug-resistant Acinetobacter baumannii. Frontiers in Cellular and Infection Microbiology, 9. ISSN 2235-2988 http://doi.org/10.3389/fcimb.2019.00203 doi:10.3389/fcimb.2019.00203 |
| spellingShingle | QH301 Biology QR Microbiology Mujawar, Shama * Mishra, Rohit Pawar, Shrikant Gatherer, Derek Lahiri, Chandrajit * Delineating the plausible molecular vaccine candidates and drug targets of multidrug-resistant Acinetobacter baumannii |
| title | Delineating the plausible molecular vaccine candidates and drug targets of multidrug-resistant Acinetobacter baumannii |
| title_full | Delineating the plausible molecular vaccine candidates and drug targets of multidrug-resistant Acinetobacter baumannii |
| title_fullStr | Delineating the plausible molecular vaccine candidates and drug targets of multidrug-resistant Acinetobacter baumannii |
| title_full_unstemmed | Delineating the plausible molecular vaccine candidates and drug targets of multidrug-resistant Acinetobacter baumannii |
| title_short | Delineating the plausible molecular vaccine candidates and drug targets of multidrug-resistant Acinetobacter baumannii |
| title_sort | delineating the plausible molecular vaccine candidates and drug targets of multidrug-resistant acinetobacter baumannii |
| topic | QH301 Biology QR Microbiology |
| url | http://eprints.sunway.edu.my/1117/ http://eprints.sunway.edu.my/1117/ http://eprints.sunway.edu.my/1117/ http://eprints.sunway.edu.my/1117/1/Lahiri%20Delineating%20the%20Plausible.pdf |