Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene glycol)-magnesium sulfate systems: Effects of molecular weights and pH
The partitioning behavior of soluble proteins from tannery wastewater using aqueous two-phase system (ATPS) was investigated. An ATPS polyethylene glycol (PEG)/MgSO4 was examined with regard to the effects of PEG molecular weight (MW) and concentration, MgSO4 concentration, pH and NaCl concentrati...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2007
|
| Subjects: | |
| Online Access: | http://scholars.utp.edu.my/id/eprint/769/ http://scholars.utp.edu.my/id/eprint/769/1/CES_-_2007.pdf |
| _version_ | 1848659037095198720 |
|---|---|
| author | Settu, sarananan J V, Rao T., Murugesan B U , Nair |
| author_facet | Settu, sarananan J V, Rao T., Murugesan B U , Nair |
| author_sort | Settu, sarananan |
| building | UTP Institutional Repository |
| collection | Online Access |
| description | The partitioning behavior of soluble proteins from tannery wastewater using aqueous two-phase system (ATPS) was investigated. An ATPS
polyethylene glycol (PEG)/MgSO4 was examined with regard to the effects of PEG molecular weight (MW) and concentration, MgSO4
concentration, pH and NaCl concentration on protein partition and extraction. The partition coefficients measured for soluble proteins were
proportional to the difference in PEG concentration between the phases. TheMWand concentration of PEG were found to have significant effects
on protein partition and extraction with low MW PEG4000 showing the best conditions for the partitioning of protein in PEG+MgSO4+water
system. Sulfate salt was chosen as the phase-forming salt because of its ability to promote hydrophobic difference between the phases. This
system was operated at room temperature (30 ◦C). Increase in pH of the system increases the partition coefficient of proteins from tannery
wastewater. The addition of sodium chloride showed significant influence on the partition coefficient. ATPS comprising PEG4000-magnesium
sulfate provided a means for the recovery of proteins from tannery wastewater. The maximum percentage yield of protein extracted is 82.68%. |
| first_indexed | 2025-11-13T07:24:03Z |
| format | Article |
| id | oai:scholars.utp.edu.my:769 |
| institution | Universiti Teknologi Petronas |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-13T07:24:03Z |
| publishDate | 2007 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:scholars.utp.edu.my:7692017-01-19T08:26:58Z http://scholars.utp.edu.my/id/eprint/769/ Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene glycol)-magnesium sulfate systems: Effects of molecular weights and pH Settu, sarananan J V, Rao T., Murugesan B U , Nair TP Chemical technology The partitioning behavior of soluble proteins from tannery wastewater using aqueous two-phase system (ATPS) was investigated. An ATPS polyethylene glycol (PEG)/MgSO4 was examined with regard to the effects of PEG molecular weight (MW) and concentration, MgSO4 concentration, pH and NaCl concentration on protein partition and extraction. The partition coefficients measured for soluble proteins were proportional to the difference in PEG concentration between the phases. TheMWand concentration of PEG were found to have significant effects on protein partition and extraction with low MW PEG4000 showing the best conditions for the partitioning of protein in PEG+MgSO4+water system. Sulfate salt was chosen as the phase-forming salt because of its ability to promote hydrophobic difference between the phases. This system was operated at room temperature (30 ◦C). Increase in pH of the system increases the partition coefficient of proteins from tannery wastewater. The addition of sodium chloride showed significant influence on the partition coefficient. ATPS comprising PEG4000-magnesium sulfate provided a means for the recovery of proteins from tannery wastewater. The maximum percentage yield of protein extracted is 82.68%. Elsevier 2007-02 Article PeerReviewed application/pdf en http://scholars.utp.edu.my/id/eprint/769/1/CES_-_2007.pdf Settu, sarananan and J V, Rao and T., Murugesan and B U , Nair (2007) Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene glycol)-magnesium sulfate systems: Effects of molecular weights and pH. Chemical Engineering Science, 62 (4). pp. 969-978. ISSN ISSN: 0009-2509 |
| spellingShingle | TP Chemical technology Settu, sarananan J V, Rao T., Murugesan B U , Nair Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene glycol)-magnesium sulfate systems: Effects of molecular weights and pH |
| title | Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene
glycol)-magnesium sulfate systems: Effects of molecular weights and pH |
| title_full | Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene
glycol)-magnesium sulfate systems: Effects of molecular weights and pH |
| title_fullStr | Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene
glycol)-magnesium sulfate systems: Effects of molecular weights and pH |
| title_full_unstemmed | Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene
glycol)-magnesium sulfate systems: Effects of molecular weights and pH |
| title_short | Partition of tannerywastewater proteins in aqueous two-phase poly (ethylene
glycol)-magnesium sulfate systems: Effects of molecular weights and pH |
| title_sort | partition of tannerywastewater proteins in aqueous two-phase poly (ethylene
glycol)-magnesium sulfate systems: effects of molecular weights and ph |
| topic | TP Chemical technology |
| url | http://scholars.utp.edu.my/id/eprint/769/ http://scholars.utp.edu.my/id/eprint/769/1/CES_-_2007.pdf |