Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system
The effect of phase forming components, pH, neutral salt (NaCl) addition on partitioning of bovine serum albumin (BSA) in PEG2000-sodium citrate based aqueous two-phase system was investigated at 25, 35, and 45C. The systems were prepared by varying the phase forming components concentration at a...
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| Format: | Article |
| Language: | English |
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2007
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| Online Access: | http://scholars.utp.edu.my/id/eprint/526/ http://scholars.utp.edu.my/id/eprint/526/1/paper.pdf |
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| author | M., Perumalsamy T., Murugesan |
| author_facet | M., Perumalsamy T., Murugesan |
| author_sort | M., Perumalsamy |
| building | UTP Institutional Repository |
| collection | Online Access |
| description | The effect of phase forming components, pH, neutral salt (NaCl) addition on partitioning of bovine serum albumin (BSA) in PEG2000-sodium citrate based aqueous two-phase system was investigated at 25, 35, and 45C. The systems were prepared by varying the phase forming components concentration at a pH of 5.0, 6.0, 7.0, 8.0, and 9.0 with different NaCl salt concentrations of 0.05M, 0.1M, 1.5M, 0.2M, 0.3M, and 0.4M. The affinity of the BSA for the lower phase increases with increase in pH, due to increase in ratio of trivalent to divalent citrate ions in the two-phase system. It was confirmed that the partition of BSA depends on the relative hydrophobicity of the compounds as well as their charge. Optimum level of pH and NaCl concentration for the partitioning of bovine serum albumin was determined.
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| first_indexed | 2025-11-13T07:23:33Z |
| format | Article |
| id | oai:scholars.utp.edu.my:526 |
| institution | Universiti Teknologi Petronas |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-13T07:23:33Z |
| publishDate | 2007 |
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| spelling | oai:scholars.utp.edu.my:5262017-01-19T08:27:06Z http://scholars.utp.edu.my/id/eprint/526/ Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system M., Perumalsamy T., Murugesan TP Chemical technology The effect of phase forming components, pH, neutral salt (NaCl) addition on partitioning of bovine serum albumin (BSA) in PEG2000-sodium citrate based aqueous two-phase system was investigated at 25, 35, and 45C. The systems were prepared by varying the phase forming components concentration at a pH of 5.0, 6.0, 7.0, 8.0, and 9.0 with different NaCl salt concentrations of 0.05M, 0.1M, 1.5M, 0.2M, 0.3M, and 0.4M. The affinity of the BSA for the lower phase increases with increase in pH, due to increase in ratio of trivalent to divalent citrate ions in the two-phase system. It was confirmed that the partition of BSA depends on the relative hydrophobicity of the compounds as well as their charge. Optimum level of pH and NaCl concentration for the partitioning of bovine serum albumin was determined. 2007 Article PeerReviewed application/pdf en http://scholars.utp.edu.my/id/eprint/526/1/paper.pdf M., Perumalsamy and T., Murugesan (2007) Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system. Separation Science and Technology, 42 (9). pp. 2049-2065. ISSN 1496395 http://www.scopus.com/inward/record.url?eid=2-s2.0-34547404303&partnerID=40&md5=371d6b4b35142ce236477645df5acaf8 10.1080/15363830701313404 10.1080/15363830701313404 10.1080/15363830701313404 |
| spellingShingle | TP Chemical technology M., Perumalsamy T., Murugesan Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system |
| title | Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system |
| title_full | Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system |
| title_fullStr | Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system |
| title_full_unstemmed | Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system |
| title_short | Partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system |
| title_sort | partition behavior of bovine serum albumin in peg2000-sodium citrate-water based aqueous two-phase system |
| topic | TP Chemical technology |
| url | http://scholars.utp.edu.my/id/eprint/526/ http://scholars.utp.edu.my/id/eprint/526/ http://scholars.utp.edu.my/id/eprint/526/ http://scholars.utp.edu.my/id/eprint/526/1/paper.pdf |