Design of Aqueous Two-Phase Protein Separation Systems
Aqueous Two-Phase System (ATPS), despite its potential as a bulk protein separation technique that offers high yield, low cost and mild environment, has limited application at industrial scale. The work proposes an integrated framework that calculates flowsheet design for the application of ATPS to...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2005
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| Online Access: | http://scholars.utp.edu.my/id/eprint/3256/ http://scholars.utp.edu.my/id/eprint/3256/1/Design_of_Aqueous_Two_Phase_Protein_Separation_Systems_%28ICBP_2005%29.pdf |
| _version_ | 1848659375085846528 |
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| author | Ahmad, Murni M |
| author_facet | Ahmad, Murni M |
| author_sort | Ahmad, Murni M |
| building | UTP Institutional Repository |
| collection | Online Access |
| description | Aqueous Two-Phase System (ATPS), despite its potential as a bulk protein separation technique that offers high yield, low cost and mild environment, has limited application at industrial scale. The work proposes an integrated framework that calculates flowsheet design for the application of ATPS to extract a target protein from cell lysate. The framework integrates the liquid-liquid equilibrium calculation based on Flory-Huggins Model and the mass balance calculation for the flowsheet. The applicability of the framework is demonstrated using a case study on a multi-stage extraction of phosphofructokinase and ovalbumin using PEG6000-DxT500 and PEG6000-Na3PO4 ATPSs. |
| first_indexed | 2025-11-13T07:29:25Z |
| format | Conference or Workshop Item |
| id | oai:scholars.utp.edu.my:3256 |
| institution | Universiti Teknologi Petronas |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-13T07:29:25Z |
| publishDate | 2005 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:scholars.utp.edu.my:32562017-01-19T08:27:28Z http://scholars.utp.edu.my/id/eprint/3256/ Design of Aqueous Two-Phase Protein Separation Systems Ahmad, Murni M TP Chemical technology Aqueous Two-Phase System (ATPS), despite its potential as a bulk protein separation technique that offers high yield, low cost and mild environment, has limited application at industrial scale. The work proposes an integrated framework that calculates flowsheet design for the application of ATPS to extract a target protein from cell lysate. The framework integrates the liquid-liquid equilibrium calculation based on Flory-Huggins Model and the mass balance calculation for the flowsheet. The applicability of the framework is demonstrated using a case study on a multi-stage extraction of phosphofructokinase and ovalbumin using PEG6000-DxT500 and PEG6000-Na3PO4 ATPSs. 2005-06 Conference or Workshop Item PeerReviewed application/pdf en http://scholars.utp.edu.my/id/eprint/3256/1/Design_of_Aqueous_Two_Phase_Protein_Separation_Systems_%28ICBP_2005%29.pdf Ahmad, Murni M (2005) Design of Aqueous Two-Phase Protein Separation Systems. In: International Conference on Biopartitioning and Purification (BPP 2005), June 2005, Amsterdam, Netherlands. |
| spellingShingle | TP Chemical technology Ahmad, Murni M Design of Aqueous Two-Phase Protein Separation Systems |
| title | Design of Aqueous Two-Phase Protein Separation Systems |
| title_full | Design of Aqueous Two-Phase Protein Separation Systems |
| title_fullStr | Design of Aqueous Two-Phase Protein Separation Systems |
| title_full_unstemmed | Design of Aqueous Two-Phase Protein Separation Systems |
| title_short | Design of Aqueous Two-Phase Protein Separation Systems |
| title_sort | design of aqueous two-phase protein separation systems |
| topic | TP Chemical technology |
| url | http://scholars.utp.edu.my/id/eprint/3256/ http://scholars.utp.edu.my/id/eprint/3256/1/Design_of_Aqueous_Two_Phase_Protein_Separation_Systems_%28ICBP_2005%29.pdf |