Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems
A comparative analysis are made to compare flowsheet designs of the application of Aqueous Two-Phase System (ATPS) to extract protein from cell lysate. The analysis that observes the protein purity and yield trends is conducted using a flowsheet calculation framework that calculates the thermodynami...
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| Format: | Conference or Workshop Item |
| Language: | English |
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2006
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| Online Access: | http://scholars.utp.edu.my/id/eprint/3232/ http://scholars.utp.edu.my/id/eprint/3232/1/Comparative_Analysis_of_Multiple_Designs_for_Aqueous_Two_Phase_Protein_Extraction_Systems_%28ARM_2006%29.pdf |
| _version_ | 1848659370178510848 |
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| author | Ahmad, Murni M |
| author_facet | Ahmad, Murni M |
| author_sort | Ahmad, Murni M |
| building | UTP Institutional Repository |
| collection | Online Access |
| description | A comparative analysis are made to compare flowsheet designs of the application of Aqueous Two-Phase System (ATPS) to extract protein from cell lysate. The analysis that observes the protein purity and yield trends is conducted using a flowsheet calculation framework that calculates the thermodynamic behaviour of the polymers and polymer-salt systems containing protein(s) within a limited range of properties and concentration. The designs include one-stage and two-stage extraction stages containing two types of ATPS i.e. PEG6000-DxT500 and PEG6000-Na3PO4 containing phosphofructokinase and ovalbumin. The results match previous findings to some extent. The analysis highlights potential for flowsheet design optimization work. |
| first_indexed | 2025-11-13T07:29:21Z |
| format | Conference or Workshop Item |
| id | oai:scholars.utp.edu.my:3232 |
| institution | Universiti Teknologi Petronas |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-13T07:29:21Z |
| publishDate | 2006 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:scholars.utp.edu.my:32322017-01-19T08:27:21Z http://scholars.utp.edu.my/id/eprint/3232/ Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems Ahmad, Murni M TP Chemical technology A comparative analysis are made to compare flowsheet designs of the application of Aqueous Two-Phase System (ATPS) to extract protein from cell lysate. The analysis that observes the protein purity and yield trends is conducted using a flowsheet calculation framework that calculates the thermodynamic behaviour of the polymers and polymer-salt systems containing protein(s) within a limited range of properties and concentration. The designs include one-stage and two-stage extraction stages containing two types of ATPS i.e. PEG6000-DxT500 and PEG6000-Na3PO4 containing phosphofructokinase and ovalbumin. The results match previous findings to some extent. The analysis highlights potential for flowsheet design optimization work. 2006-02 Conference or Workshop Item PeerReviewed application/pdf en http://scholars.utp.edu.my/id/eprint/3232/1/Comparative_Analysis_of_Multiple_Designs_for_Aqueous_Two_Phase_Protein_Extraction_Systems_%28ARM_2006%29.pdf Ahmad, Murni M (2006) Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems. In: Annual Review Meeting (ARM 2006), February 2006, Pittsburgh, USA. |
| spellingShingle | TP Chemical technology Ahmad, Murni M Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems |
| title | Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems |
| title_full | Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems |
| title_fullStr | Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems |
| title_full_unstemmed | Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems |
| title_short | Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems |
| title_sort | comparative analysis of multiple designs for aqueous two-phase protein extraction systems |
| topic | TP Chemical technology |
| url | http://scholars.utp.edu.my/id/eprint/3232/ http://scholars.utp.edu.my/id/eprint/3232/1/Comparative_Analysis_of_Multiple_Designs_for_Aqueous_Two_Phase_Protein_Extraction_Systems_%28ARM_2006%29.pdf |