Flowsheet simulation of aqueous two-phase extraction systems for protein purification
BACKGROUND: Aqueous two-phase extraction (ATPE) has many advantages as an efficient, inexpensive large-scale liquid–liquid extraction technique for protein separation. However, the realization of ATPE as a protein separation technology at industrial scales is rather limited due to the large, multidi...
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| Format: | Citation Index Journal |
| Language: | English |
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John Wiley & Sons, Ltd.
2010
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| Online Access: | http://scholars.utp.edu.my/id/eprint/2946/ http://scholars.utp.edu.my/id/eprint/2946/1/Flowsheet_simulation_of_aqueous_two_phase_extraction_systems_for_protein_purification.PDF |
| _version_ | 1848659329775828992 |
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| author | Ahmad, Murni M |
| author_facet | Ahmad, Murni M |
| author_sort | Ahmad, Murni M |
| building | UTP Institutional Repository |
| collection | Online Access |
| description | BACKGROUND: Aqueous two-phase extraction (ATPE) has many advantages as an efficient, inexpensive large-scale liquid–liquid extraction technique for protein separation. However, the realization of ATPE as a protein separation technology at industrial scales is rather limited due to the large, multidimensional design space and the paucity of design approaches to predict phase and product behavior in an integrated fashion with overall system performance. This paper describes a framework designed to calculate suitable flowsheets for the extraction of a target protein from a complex protein feed using ATPE. The framework incorporated a routine to set up flowsheets according to target protein partitioning behavior in specific ATPE systems and a calculation of the amounts of phase-forming components needed to extract the target protein. The thermodynamics of phase formation and partitioning were modeled using Flory-Huggins theory and calculated using a Gibbs energy difference minimization approach.
RESULTS: As a case study, suitable flowsheets to recover phosphofructokinase from a simple model feedstock using poly(ethylene glycol)-dextran (PEG6000-DxT500) and poly(ethylene glycol)-salt (PEG6000-Na3PO4) two-phase systems were designed and the existence of feasible solutions was demonstrated. The flowsheets were compared in terms of product yield, product purity, phase settling rate and scaled process cost. The effect of the mass flowrates of phase-forming components on product yield and purity was also determined.
CONCLUSION: This framework is proposed as a basis for flowsheet optimization for protein purification using ATPE systems. Copyright |
| first_indexed | 2025-11-13T07:28:42Z |
| format | Citation Index Journal |
| id | oai:scholars.utp.edu.my:2946 |
| institution | Universiti Teknologi Petronas |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-13T07:28:42Z |
| publishDate | 2010 |
| publisher | John Wiley & Sons, Ltd. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:scholars.utp.edu.my:29462017-01-19T08:23:51Z http://scholars.utp.edu.my/id/eprint/2946/ Flowsheet simulation of aqueous two-phase extraction systems for protein purification Ahmad, Murni M TP Chemical technology BACKGROUND: Aqueous two-phase extraction (ATPE) has many advantages as an efficient, inexpensive large-scale liquid–liquid extraction technique for protein separation. However, the realization of ATPE as a protein separation technology at industrial scales is rather limited due to the large, multidimensional design space and the paucity of design approaches to predict phase and product behavior in an integrated fashion with overall system performance. This paper describes a framework designed to calculate suitable flowsheets for the extraction of a target protein from a complex protein feed using ATPE. The framework incorporated a routine to set up flowsheets according to target protein partitioning behavior in specific ATPE systems and a calculation of the amounts of phase-forming components needed to extract the target protein. The thermodynamics of phase formation and partitioning were modeled using Flory-Huggins theory and calculated using a Gibbs energy difference minimization approach. RESULTS: As a case study, suitable flowsheets to recover phosphofructokinase from a simple model feedstock using poly(ethylene glycol)-dextran (PEG6000-DxT500) and poly(ethylene glycol)-salt (PEG6000-Na3PO4) two-phase systems were designed and the existence of feasible solutions was demonstrated. The flowsheets were compared in terms of product yield, product purity, phase settling rate and scaled process cost. The effect of the mass flowrates of phase-forming components on product yield and purity was also determined. CONCLUSION: This framework is proposed as a basis for flowsheet optimization for protein purification using ATPE systems. Copyright John Wiley & Sons, Ltd. 2010-08-02 Citation Index Journal PeerReviewed application/pdf en http://scholars.utp.edu.my/id/eprint/2946/1/Flowsheet_simulation_of_aqueous_two_phase_extraction_systems_for_protein_purification.PDF Ahmad, Murni M (2010) Flowsheet simulation of aqueous two-phase extraction systems for protein purification. [Citation Index Journal] http://dx.doi.org/10.1002/jctb.2469 10.1002/jctb.2469 10.1002/jctb.2469 10.1002/jctb.2469 |
| spellingShingle | TP Chemical technology Ahmad, Murni M Flowsheet simulation of aqueous two-phase extraction systems for protein purification |
| title | Flowsheet simulation of aqueous two-phase extraction systems for protein purification |
| title_full | Flowsheet simulation of aqueous two-phase extraction systems for protein purification |
| title_fullStr | Flowsheet simulation of aqueous two-phase extraction systems for protein purification |
| title_full_unstemmed | Flowsheet simulation of aqueous two-phase extraction systems for protein purification |
| title_short | Flowsheet simulation of aqueous two-phase extraction systems for protein purification |
| title_sort | flowsheet simulation of aqueous two-phase extraction systems for protein purification |
| topic | TP Chemical technology |
| url | http://scholars.utp.edu.my/id/eprint/2946/ http://scholars.utp.edu.my/id/eprint/2946/ http://scholars.utp.edu.my/id/eprint/2946/ http://scholars.utp.edu.my/id/eprint/2946/1/Flowsheet_simulation_of_aqueous_two_phase_extraction_systems_for_protein_purification.PDF |