Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment
Response surface methodology (RSM) was employed to optimize nitrogen and phosphorus concentrations for removal of n-alkanes from crude oil contaminated seawater samples in batch reactors. Erlenmeyer flasks were used as bioreactors; each containing 250mL dispersed crude oil contaminated seawater, in...
| Main Authors: | , , , , , |
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| Format: | Citation Index Journal |
| Language: | English |
| Published: |
Elseviers
2010
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| Subjects: | |
| Online Access: | http://scholars.utp.edu.my/id/eprint/2937/ http://scholars.utp.edu.my/id/eprint/2937/1/Application_of_statistical_experimental_methodology_to_optimize_bioremediation_of_n-alkanes_in_aquatic_environment.pdf |
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| author | Zahed, M.A. Aziz, H.A. Mohajeri, L. Mohajeri, S. Kutty, S.R.M. Isa, M.H. |
| author_facet | Zahed, M.A. Aziz, H.A. Mohajeri, L. Mohajeri, S. Kutty, S.R.M. Isa, M.H. |
| author_sort | Zahed, M.A. |
| building | UTP Institutional Repository |
| collection | Online Access |
| description | Response surface methodology (RSM) was employed to optimize nitrogen and phosphorus concentrations for removal of n-alkanes from crude oil contaminated seawater samples in batch reactors. Erlenmeyer flasks were used as bioreactors; each containing 250mL dispersed crude oil contaminated
seawater, indigenous acclimatized microorganism and different amounts of nitrogen and phosphorus based on central composite design (CCD). Samples were extracted and analyzed according to US-EPA protocols using a gas chromatograph. During 28 days of bioremediation, a maximum of 95% total aliphatic hydrocarbons removal was observed. The obtained Model F-value of 267.73 and probability F < 0.0001 implied the model was significant. Numerical condition optimization via a quadratic model, predicted
98% n-alkanes removal for a 20-day laboratory bioremediation trial using nitrogen and phosphorus concentrations of 13.62 and 1.39 mg/L, respectively. In actual experiments, 95% removal was observed under these conditions. |
| first_indexed | 2025-11-13T07:28:40Z |
| format | Citation Index Journal |
| id | oai:scholars.utp.edu.my:2937 |
| institution | Universiti Teknologi Petronas |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-13T07:28:40Z |
| publishDate | 2010 |
| publisher | Elseviers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:scholars.utp.edu.my:29372017-03-20T01:59:48Z http://scholars.utp.edu.my/id/eprint/2937/ Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment Zahed, M.A. Aziz, H.A. Mohajeri, L. Mohajeri, S. Kutty, S.R.M. Isa, M.H. TD Environmental technology. Sanitary engineering Response surface methodology (RSM) was employed to optimize nitrogen and phosphorus concentrations for removal of n-alkanes from crude oil contaminated seawater samples in batch reactors. Erlenmeyer flasks were used as bioreactors; each containing 250mL dispersed crude oil contaminated seawater, indigenous acclimatized microorganism and different amounts of nitrogen and phosphorus based on central composite design (CCD). Samples were extracted and analyzed according to US-EPA protocols using a gas chromatograph. During 28 days of bioremediation, a maximum of 95% total aliphatic hydrocarbons removal was observed. The obtained Model F-value of 267.73 and probability F < 0.0001 implied the model was significant. Numerical condition optimization via a quadratic model, predicted 98% n-alkanes removal for a 20-day laboratory bioremediation trial using nitrogen and phosphorus concentrations of 13.62 and 1.39 mg/L, respectively. In actual experiments, 95% removal was observed under these conditions. Elseviers 2010-12 Citation Index Journal PeerReviewed application/pdf en http://scholars.utp.edu.my/id/eprint/2937/1/Application_of_statistical_experimental_methodology_to_optimize_bioremediation_of_n-alkanes_in_aquatic_environment.pdf Zahed, M.A. and Aziz, H.A. and Mohajeri, L. and Mohajeri, S. and Kutty, S.R.M. and Isa, M.H. (2010) Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment. [Citation Index Journal] |
| spellingShingle | TD Environmental technology. Sanitary engineering Zahed, M.A. Aziz, H.A. Mohajeri, L. Mohajeri, S. Kutty, S.R.M. Isa, M.H. Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment |
| title | Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment |
| title_full | Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment |
| title_fullStr | Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment |
| title_full_unstemmed | Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment |
| title_short | Application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment |
| title_sort | application of statistical experimental methodology to optimize bioremediation of n-alkanes in aquatic environment |
| topic | TD Environmental technology. Sanitary engineering |
| url | http://scholars.utp.edu.my/id/eprint/2937/ http://scholars.utp.edu.my/id/eprint/2937/1/Application_of_statistical_experimental_methodology_to_optimize_bioremediation_of_n-alkanes_in_aquatic_environment.pdf |