Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
Continuous removal of anthraquinones (AQ) by Amberlite polymeric adsorbents (XAD-4, XAD-7 and XAD-16) through in situ adsorption in Morinda elliptica cell suspension cultures is studied for product recovery and improvement of the overall titre. Ethanol was the best eluting solvent for effective reco...
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| Format: | Article |
| Language: | English |
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2007
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| Online Access: | http://scholars.utp.edu.my/id/eprint/235/ http://scholars.utp.edu.my/id/eprint/235/1/paper.pdf |
| _version_ | 1848658937754157056 |
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| author | M.A., Abdullah L., Chiang |
| author_facet | M.A., Abdullah L., Chiang |
| author_sort | M.A., Abdullah |
| building | UTP Institutional Repository |
| collection | Online Access |
| description | Continuous removal of anthraquinones (AQ) by Amberlite polymeric adsorbents (XAD-4, XAD-7 and XAD-16) through in situ adsorption in Morinda elliptica cell suspension cultures is studied for product recovery and improvement of the overall titre. Ethanol was the best eluting solvent for effective recovery of AQ from all adsorbents. Pre-treatment of XAD-4 with sodium acetate not only enhanced intracellular AQ, but also AQ release and subsequent recovery from the adsorbent. The addition of sodium acetate pretreated XAD-4 on day 18 for 6-day contact period, achieved comparable cell growth to control (41 g/L), but with 1.3-fold higher intracellular AQ (124 mg/g DW) and two-fold increase in extracellular AQ (14.3 mg/L). High amount of adsorbent and longer contact period for the cultures entering stationary growth phase, stimulated AQ release and recovery but at the expense of cell growth. With 5-8.3 g XAD-4 adsorbent per litre M. elliptica culture in production (P) medium, between 60 and 90% AQ was recovered from extracellular AQ after 24-26 days of culture period. © 2007 Elsevier Ltd. All rights reserved.
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| institution | Universiti Teknologi Petronas |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-13T07:22:28Z |
| publishDate | 2007 |
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| spelling | oai:scholars.utp.edu.my:2352017-01-19T08:27:06Z http://scholars.utp.edu.my/id/eprint/235/ Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy M.A., Abdullah L., Chiang TP Chemical technology Continuous removal of anthraquinones (AQ) by Amberlite polymeric adsorbents (XAD-4, XAD-7 and XAD-16) through in situ adsorption in Morinda elliptica cell suspension cultures is studied for product recovery and improvement of the overall titre. Ethanol was the best eluting solvent for effective recovery of AQ from all adsorbents. Pre-treatment of XAD-4 with sodium acetate not only enhanced intracellular AQ, but also AQ release and subsequent recovery from the adsorbent. The addition of sodium acetate pretreated XAD-4 on day 18 for 6-day contact period, achieved comparable cell growth to control (41 g/L), but with 1.3-fold higher intracellular AQ (124 mg/g DW) and two-fold increase in extracellular AQ (14.3 mg/L). High amount of adsorbent and longer contact period for the cultures entering stationary growth phase, stimulated AQ release and recovery but at the expense of cell growth. With 5-8.3 g XAD-4 adsorbent per litre M. elliptica culture in production (P) medium, between 60 and 90% AQ was recovered from extracellular AQ after 24-26 days of culture period. © 2007 Elsevier Ltd. All rights reserved. 2007 Article NonPeerReviewed application/pdf en http://scholars.utp.edu.my/id/eprint/235/1/paper.pdf M.A., Abdullah and L., Chiang (2007) Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy. Process Biochemistry, 42 (5). pp. 757-763. ISSN 13595113 http://www.scopus.com/inward/record.url?eid=2-s2.0-34047104562&partnerID=40&md5=dbcbb1c43e429828f8b0003089f80106 10.1016/j.procbio.2007.01.005 10.1016/j.procbio.2007.01.005 10.1016/j.procbio.2007.01.005 |
| spellingShingle | TP Chemical technology M.A., Abdullah L., Chiang Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy |
| title | Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
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| title_full | Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
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| title_fullStr | Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
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| title_full_unstemmed | Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
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| title_short | Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
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| title_sort | enhanced anthraquinones production from adsorbent-treated morinda elliptica cell suspension cultures in production medium strategy |
| topic | TP Chemical technology |
| url | http://scholars.utp.edu.my/id/eprint/235/ http://scholars.utp.edu.my/id/eprint/235/ http://scholars.utp.edu.my/id/eprint/235/ http://scholars.utp.edu.my/id/eprint/235/1/paper.pdf |