Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production
Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2016
|
| Online Access: | http://journalarticle.ukm.my/9886/ http://journalarticle.ukm.my/9886/1/18_Sheril_Norliana_Suhaimi.pdf |
| _version_ | 1848812218704986112 |
|---|---|
| author | Sheril Norliana Suhaimi, Nur Amelia Azreen Adnan, Yee, Phang Lai Suraini Abd-Aziz, Maeda, Toshinari |
| author_facet | Sheril Norliana Suhaimi, Nur Amelia Azreen Adnan, Yee, Phang Lai Suraini Abd-Aziz, Maeda, Toshinari |
| author_sort | Sheril Norliana Suhaimi, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified based on enzymatic oxidation of ethanol, which is correlated to reduction of 2,6-dichlorophenol-indophenol dye that resulted in the formation of yellow zone. Approximately 300 colonies were able to grow on minimal media using glycerol as sole carbon. Only about 70 isolates showed positive result when using the modified ethanol production assay after pre-screening stage. The formation of decolourized zone was apparent using modified assay containing 5 mL/L of 0.05M 2,6-dichlorophenol-indophenol, 10 mL of reaction mixture and 500 μl/L of enzyme, respectively. The ethanol production capability of the isolates was further proven by anaerobic fermentation as a quantitative method. This modified method is applicable in screening for ethanol producer from glycerol as carbon source allows rapid and more bacteria can be screened. |
| first_indexed | 2025-11-14T23:58:48Z |
| format | Article |
| id | oai:generic.eprints.org:9886 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:58:48Z |
| publishDate | 2016 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:98862016-12-14T06:51:09Z http://journalarticle.ukm.my/9886/ Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production Sheril Norliana Suhaimi, Nur Amelia Azreen Adnan, Yee, Phang Lai Suraini Abd-Aziz, Maeda, Toshinari Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified based on enzymatic oxidation of ethanol, which is correlated to reduction of 2,6-dichlorophenol-indophenol dye that resulted in the formation of yellow zone. Approximately 300 colonies were able to grow on minimal media using glycerol as sole carbon. Only about 70 isolates showed positive result when using the modified ethanol production assay after pre-screening stage. The formation of decolourized zone was apparent using modified assay containing 5 mL/L of 0.05M 2,6-dichlorophenol-indophenol, 10 mL of reaction mixture and 500 μl/L of enzyme, respectively. The ethanol production capability of the isolates was further proven by anaerobic fermentation as a quantitative method. This modified method is applicable in screening for ethanol producer from glycerol as carbon source allows rapid and more bacteria can be screened. Penerbit Universiti Kebangsaan Malaysia 2016-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9886/1/18_Sheril_Norliana_Suhaimi.pdf Sheril Norliana Suhaimi, and Nur Amelia Azreen Adnan, and Yee, Phang Lai and Suraini Abd-Aziz, and Maeda, Toshinari (2016) Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production. Sains Malaysiana, 45 (5). pp. 811-815. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol45num5_2016/contentsVol45num5_2016.html |
| spellingShingle | Sheril Norliana Suhaimi, Nur Amelia Azreen Adnan, Yee, Phang Lai Suraini Abd-Aziz, Maeda, Toshinari Rapid screening method for isolation of glycerol-consuming bacteria for ethanol production |
| title | Rapid screening method for isolation of glycerol-consuming
bacteria for ethanol production |
| title_full | Rapid screening method for isolation of glycerol-consuming
bacteria for ethanol production |
| title_fullStr | Rapid screening method for isolation of glycerol-consuming
bacteria for ethanol production |
| title_full_unstemmed | Rapid screening method for isolation of glycerol-consuming
bacteria for ethanol production |
| title_short | Rapid screening method for isolation of glycerol-consuming
bacteria for ethanol production |
| title_sort | rapid screening method for isolation of glycerol-consuming
bacteria for ethanol production |
| url | http://journalarticle.ukm.my/9886/ http://journalarticle.ukm.my/9886/ http://journalarticle.ukm.my/9886/1/18_Sheril_Norliana_Suhaimi.pdf |