Studies on separation and properties of lumbrokinase in pheretima praepinguis
In order to separate lumbrokinase in Pheretima praepinguis and to study the enzymatic properties, with Pheretima praepinguis as material, lumbrokinase was separated with the salting out method. Lumbrokinase activity was measured with casein medium plate method. The effect of pH and temperature on lu...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2016
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| Online Access: | http://journalarticle.ukm.my/9576/ http://journalarticle.ukm.my/9576/1/14_Tang_Mei.pdf |
| _version_ | 1848812134939492352 |
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| author | Tang, Mei Liu, Cao Liang, Zi Gong, Mingfu Hu, Die |
| author_facet | Tang, Mei Liu, Cao Liang, Zi Gong, Mingfu Hu, Die |
| author_sort | Tang, Mei |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | In order to separate lumbrokinase in Pheretima praepinguis and to study the enzymatic properties, with Pheretima praepinguis as material, lumbrokinase was separated with the salting out method. Lumbrokinase activity was measured with casein medium plate method. The effect of pH and temperature on lumbrokinase activity was studied. The activity of lumbrokinase separated from Pheretima praepinguis was relatively high. Lumbrokinase activity in neutral or slightly alkaline environment was higher. Lumbrokinase had tolerance ability to high temperature, with highly enzymatic activity under 60° and wide range of temperature adaptation. |
| first_indexed | 2025-11-14T23:57:28Z |
| format | Article |
| id | oai:generic.eprints.org:9576 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:57:28Z |
| publishDate | 2016 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:95762016-12-14T06:50:21Z http://journalarticle.ukm.my/9576/ Studies on separation and properties of lumbrokinase in pheretima praepinguis Tang, Mei Liu, Cao Liang, Zi Gong, Mingfu Hu, Die In order to separate lumbrokinase in Pheretima praepinguis and to study the enzymatic properties, with Pheretima praepinguis as material, lumbrokinase was separated with the salting out method. Lumbrokinase activity was measured with casein medium plate method. The effect of pH and temperature on lumbrokinase activity was studied. The activity of lumbrokinase separated from Pheretima praepinguis was relatively high. Lumbrokinase activity in neutral or slightly alkaline environment was higher. Lumbrokinase had tolerance ability to high temperature, with highly enzymatic activity under 60° and wide range of temperature adaptation. Universiti Kebangsaan Malaysia 2016-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9576/1/14_Tang_Mei.pdf Tang, Mei and Liu, Cao and Liang, Zi and Gong, Mingfu and Hu, Die (2016) Studies on separation and properties of lumbrokinase in pheretima praepinguis. Sains Malaysiana, 45 (1). pp. 115-118. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid45bil1_2016/KandunganJilid45Bil1_2016.htm |
| spellingShingle | Tang, Mei Liu, Cao Liang, Zi Gong, Mingfu Hu, Die Studies on separation and properties of lumbrokinase in pheretima praepinguis |
| title | Studies on separation and properties of lumbrokinase in pheretima praepinguis |
| title_full | Studies on separation and properties of lumbrokinase in pheretima praepinguis |
| title_fullStr | Studies on separation and properties of lumbrokinase in pheretima praepinguis |
| title_full_unstemmed | Studies on separation and properties of lumbrokinase in pheretima praepinguis |
| title_short | Studies on separation and properties of lumbrokinase in pheretima praepinguis |
| title_sort | studies on separation and properties of lumbrokinase in pheretima praepinguis |
| url | http://journalarticle.ukm.my/9576/ http://journalarticle.ukm.my/9576/ http://journalarticle.ukm.my/9576/1/14_Tang_Mei.pdf |