Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic acid by supercritical carbon dioxide technology using response surface methodology

γ-linolenic acid is an essential omega-6 unsaturated fatty acid made in the human body from linoleic acid. It can be metabolized to various important eicosanoids and it is also a precursor of prostaglandin E and several other active substances that are associated with anti-inflammatory properties. A...

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Main Authors: Yao, Ching Hsueh, Be, Jen Wang, Zer, Ran Yu, Cheng, Chi Wang, Malcolm Koo
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2015
Online Access:http://journalarticle.ukm.my/9496/
http://journalarticle.ukm.my/9496/1/12_Yao-Chang_Hsueh.pdf
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author Yao, Ching Hsueh
Be, Jen Wang
Zer, Ran Yu
Cheng, Chi Wang
Malcolm Koo,
author_facet Yao, Ching Hsueh
Be, Jen Wang
Zer, Ran Yu
Cheng, Chi Wang
Malcolm Koo,
author_sort Yao, Ching Hsueh
building UKM Institutional Repository
collection Online Access
description γ-linolenic acid is an essential omega-6 unsaturated fatty acid made in the human body from linoleic acid. It can be metabolized to various important eicosanoids and it is also a precursor of prostaglandin E and several other active substances that are associated with anti-inflammatory properties. Arthrospira platensis is known to contain relatively large quantities of γ-linolenic acid. The aim of this study was to investigate the optimal parameters under a continuous preparation method of γ-linolenic acid from A. platensis using supercritical carbon dioxide technology. A Box-Behnken experimental design and response surface methodology were used to optimize combinations among pressure (10, 20 and 30 MPa), temperature (40, 50 and 60°C) and flow rate of A. platensis extract liquor (1, 2 and 3 mL/min) for yield of γ-linolenic acid. The results showed that the extraction of γ-linolenic acid from A. platensis was optimized at a temperature of 60°C, a pressure of 30 MPa and a flow rate of 3 mL/min. These parameters could be used as a basis for facilitating future scale-up industrial applications.
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spelling oai:generic.eprints.org:94962016-12-14T06:50:06Z http://journalarticle.ukm.my/9496/ Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic acid by supercritical carbon dioxide technology using response surface methodology Yao, Ching Hsueh Be, Jen Wang Zer, Ran Yu Cheng, Chi Wang Malcolm Koo, γ-linolenic acid is an essential omega-6 unsaturated fatty acid made in the human body from linoleic acid. It can be metabolized to various important eicosanoids and it is also a precursor of prostaglandin E and several other active substances that are associated with anti-inflammatory properties. Arthrospira platensis is known to contain relatively large quantities of γ-linolenic acid. The aim of this study was to investigate the optimal parameters under a continuous preparation method of γ-linolenic acid from A. platensis using supercritical carbon dioxide technology. A Box-Behnken experimental design and response surface methodology were used to optimize combinations among pressure (10, 20 and 30 MPa), temperature (40, 50 and 60°C) and flow rate of A. platensis extract liquor (1, 2 and 3 mL/min) for yield of γ-linolenic acid. The results showed that the extraction of γ-linolenic acid from A. platensis was optimized at a temperature of 60°C, a pressure of 30 MPa and a flow rate of 3 mL/min. These parameters could be used as a basis for facilitating future scale-up industrial applications. Universiti Kebangsaan Malaysia 2015-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9496/1/12_Yao-Chang_Hsueh.pdf Yao, Ching Hsueh and Be, Jen Wang and Zer, Ran Yu and Cheng, Chi Wang and Malcolm Koo, (2015) Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic acid by supercritical carbon dioxide technology using response surface methodology. Sains Malaysiana, 44 (12). pp. 1739-1744. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil12_2015/KandunganJilid44Bil12_2015.html
spellingShingle Yao, Ching Hsueh
Be, Jen Wang
Zer, Ran Yu
Cheng, Chi Wang
Malcolm Koo,
Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic acid by supercritical carbon dioxide technology using response surface methodology
title Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic acid by supercritical carbon dioxide technology using response surface methodology
title_full Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic acid by supercritical carbon dioxide technology using response surface methodology
title_fullStr Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic acid by supercritical carbon dioxide technology using response surface methodology
title_full_unstemmed Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic acid by supercritical carbon dioxide technology using response surface methodology
title_short Optimization of a Continuous Preparation Method of Arthrospira platensis γ-linolenic acid by supercritical carbon dioxide technology using response surface methodology
title_sort optimization of a continuous preparation method of arthrospira platensis γ-linolenic acid by supercritical carbon dioxide technology using response surface methodology
url http://journalarticle.ukm.my/9496/
http://journalarticle.ukm.my/9496/
http://journalarticle.ukm.my/9496/1/12_Yao-Chang_Hsueh.pdf