Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa
The objectives of this study were to determine the effect of different cryoprotectants and sperm densities for long-term storage of orange mud crab, Scylla olivacea spermatozoa. Spermatozoa were obtained by homogenizing the spermatophores using a glass homogenizer in an ice-bath followed by centrifu...
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2015
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| Online Access: | http://journalarticle.ukm.my/9263/ http://journalarticle.ukm.my/9263/1/10_Mhd_Ikhwanuddin.pdf |
| _version_ | 1848812029836525568 |
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| author | Mhd Ikhwanuddin, Teng Phei Yin, Abdul Jabbar Menon, Safiah Jasmani, Ambok Bolong Abol-Munafi, |
| author_facet | Mhd Ikhwanuddin, Teng Phei Yin, Abdul Jabbar Menon, Safiah Jasmani, Ambok Bolong Abol-Munafi, |
| author_sort | Mhd Ikhwanuddin, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The objectives of this study were to determine the effect of different cryoprotectants and sperm densities for long-term storage of orange mud crab, Scylla olivacea spermatozoa. Spermatozoa were obtained by homogenizing the spermatophores using a glass homogenizer in an ice-bath followed by centrifugation at 4°C. Spermatozoa were then suspended in calcium-free saline (Ca-F saline) containing 5% of the following cryoprotectants: Glycerol, dimethyl sulfoxide (DMSO) and methanol. Sperm which vibrated and rotated were counted as live during sperm viability assessment. Samples of spermatozoa were cooled to -196°C by two-step freezing, first to -80°C and then by plunging into liquid nitrogen (LN). Spermatozoa were gradually cooled at 1°C/min. Thawing was carried out in a 30°C water bath for 2 min. This yielded live sperm after storage in LN for 30 days. The best sperm viability was obtained from a density of 108 cells per mL in DMSO. There was no significant difference (p>0.05) among cryoprotectants toward sperm viability. However, sperm viability was significantly affected (p>0.05) by cell densities. In conclusion, DMSO gave the best protection to sperm cells of S. olivacea, but the effectiveness of DMSO as a cryoprotectant is influenced by sperm density. |
| first_indexed | 2025-11-14T23:55:48Z |
| format | Article |
| id | oai:generic.eprints.org:9263 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:55:48Z |
| publishDate | 2015 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:92632016-12-14T06:49:23Z http://journalarticle.ukm.my/9263/ Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa Mhd Ikhwanuddin, Teng Phei Yin, Abdul Jabbar Menon, Safiah Jasmani, Ambok Bolong Abol-Munafi, The objectives of this study were to determine the effect of different cryoprotectants and sperm densities for long-term storage of orange mud crab, Scylla olivacea spermatozoa. Spermatozoa were obtained by homogenizing the spermatophores using a glass homogenizer in an ice-bath followed by centrifugation at 4°C. Spermatozoa were then suspended in calcium-free saline (Ca-F saline) containing 5% of the following cryoprotectants: Glycerol, dimethyl sulfoxide (DMSO) and methanol. Sperm which vibrated and rotated were counted as live during sperm viability assessment. Samples of spermatozoa were cooled to -196°C by two-step freezing, first to -80°C and then by plunging into liquid nitrogen (LN). Spermatozoa were gradually cooled at 1°C/min. Thawing was carried out in a 30°C water bath for 2 min. This yielded live sperm after storage in LN for 30 days. The best sperm viability was obtained from a density of 108 cells per mL in DMSO. There was no significant difference (p>0.05) among cryoprotectants toward sperm viability. However, sperm viability was significantly affected (p>0.05) by cell densities. In conclusion, DMSO gave the best protection to sperm cells of S. olivacea, but the effectiveness of DMSO as a cryoprotectant is influenced by sperm density. Universiti Kebangsaan Malaysia 2015-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/9263/1/10_Mhd_Ikhwanuddin.pdf Mhd Ikhwanuddin, and Teng Phei Yin, and Abdul Jabbar Menon, and Safiah Jasmani, and Ambok Bolong Abol-Munafi, (2015) Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa. Sains Malaysiana, 44 (9). pp. 1283-1288. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil9_2015/KandunganJilid44Bil9_2015.html |
| spellingShingle | Mhd Ikhwanuddin, Teng Phei Yin, Abdul Jabbar Menon, Safiah Jasmani, Ambok Bolong Abol-Munafi, Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa |
| title | Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa |
| title_full | Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa |
| title_fullStr | Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa |
| title_full_unstemmed | Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa |
| title_short | Effect of different cryoprotectants and sperm densities of orange mud crab, Scylla olivacea (Herbst, 1796) for long-term storage of spermatozoa |
| title_sort | effect of different cryoprotectants and sperm densities of orange mud crab, scylla olivacea (herbst, 1796) for long-term storage of spermatozoa |
| url | http://journalarticle.ukm.my/9263/ http://journalarticle.ukm.my/9263/ http://journalarticle.ukm.my/9263/1/10_Mhd_Ikhwanuddin.pdf |