Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia
Eurycoma longifolia is one of the well-known herbal plants in Southeast Asian region due to its remarkable properties, especially the root part that can be used as aphrodisiac, anti-cancer, anti-malaria and anti-ulcer agent. Uncontrolled harvesting of this plant has reduced its population. Thus, an...
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2015
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| Online Access: | http://journalarticle.ukm.my/8974/ http://journalarticle.ukm.my/8974/1/01_Zawawi_Dhiya.pdf |
| _version_ | 1848811940549230592 |
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| author | Zawawi Dhiya Dalila, Ja’afar Hafsah, Zainuddin Rokiah, Kari Rodziah, Mohd Noor Madihah, |
| author_facet | Zawawi Dhiya Dalila, Ja’afar Hafsah, Zainuddin Rokiah, Kari Rodziah, Mohd Noor Madihah, |
| author_sort | Zawawi Dhiya Dalila, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Eurycoma longifolia is one of the well-known herbal plants in Southeast Asian region due to its remarkable properties, especially the root part that can be used as aphrodisiac, anti-cancer, anti-malaria and anti-ulcer agent. Uncontrolled harvesting of this plant has reduced its population. Thus, an efficient protocol involving the induction of direct somatic embryogenesis from cotyledon culture of E. longifolia has been developed. 15% explants forming embryos were achieved on Modified Murashige and Skoog’s (MMS) medium supplemented with 0.1 mg/L zeatin plus 0.2 mg/L indole-3-butyric acid (IBA). However, the addition of 0.12 mg/L thidiazuron (TDZ) has increased the percentage up to 22.0%. The maturation phase occurred in the same medium, thus decreased the frequency of subculture and the genetic instability of the embryos. Secondary somatic embryogenesis growth of 20%, which was the highest percentage of growth, was achieved from the primary embryos that were cultured in the MMS medium with the treatment of 2.0 mg/L IBA plus 0.075 mg/L TDZ. The results indicated that the direct somatic embryogenesis obtained can be further used to develop a protocol for plantlet regeneration of E. longifolia. |
| first_indexed | 2025-11-14T23:54:23Z |
| format | Article |
| id | oai:generic.eprints.org:8974 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:54:23Z |
| publishDate | 2015 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:89742016-12-14T06:48:36Z http://journalarticle.ukm.my/8974/ Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia Zawawi Dhiya Dalila, Ja’afar Hafsah, Zainuddin Rokiah, Kari Rodziah, Mohd Noor Madihah, Eurycoma longifolia is one of the well-known herbal plants in Southeast Asian region due to its remarkable properties, especially the root part that can be used as aphrodisiac, anti-cancer, anti-malaria and anti-ulcer agent. Uncontrolled harvesting of this plant has reduced its population. Thus, an efficient protocol involving the induction of direct somatic embryogenesis from cotyledon culture of E. longifolia has been developed. 15% explants forming embryos were achieved on Modified Murashige and Skoog’s (MMS) medium supplemented with 0.1 mg/L zeatin plus 0.2 mg/L indole-3-butyric acid (IBA). However, the addition of 0.12 mg/L thidiazuron (TDZ) has increased the percentage up to 22.0%. The maturation phase occurred in the same medium, thus decreased the frequency of subculture and the genetic instability of the embryos. Secondary somatic embryogenesis growth of 20%, which was the highest percentage of growth, was achieved from the primary embryos that were cultured in the MMS medium with the treatment of 2.0 mg/L IBA plus 0.075 mg/L TDZ. The results indicated that the direct somatic embryogenesis obtained can be further used to develop a protocol for plantlet regeneration of E. longifolia. Universiti Kebangsaan Malaysia 2015-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/8974/1/01_Zawawi_Dhiya.pdf Zawawi Dhiya Dalila, and Ja’afar Hafsah, and Zainuddin Rokiah, and Kari Rodziah, and Mohd Noor Madihah, (2015) Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia. Sains Malaysiana, 44 (7). pp. 913-920. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid44bil7_2015/KandunganJilid44Bil7_2015.html |
| spellingShingle | Zawawi Dhiya Dalila, Ja’afar Hafsah, Zainuddin Rokiah, Kari Rodziah, Mohd Noor Madihah, Thidiazuron induces high frequency direct somatic embryogenesis growth from cotyledon culture of eurycoma longifolia |
| title | Thidiazuron induces high frequency direct somatic embryogenesis
growth from cotyledon culture of eurycoma longifolia |
| title_full | Thidiazuron induces high frequency direct somatic embryogenesis
growth from cotyledon culture of eurycoma longifolia |
| title_fullStr | Thidiazuron induces high frequency direct somatic embryogenesis
growth from cotyledon culture of eurycoma longifolia |
| title_full_unstemmed | Thidiazuron induces high frequency direct somatic embryogenesis
growth from cotyledon culture of eurycoma longifolia |
| title_short | Thidiazuron induces high frequency direct somatic embryogenesis
growth from cotyledon culture of eurycoma longifolia |
| title_sort | thidiazuron induces high frequency direct somatic embryogenesis
growth from cotyledon culture of eurycoma longifolia |
| url | http://journalarticle.ukm.my/8974/ http://journalarticle.ukm.my/8974/ http://journalarticle.ukm.my/8974/1/01_Zawawi_Dhiya.pdf |