Characterisation and stabilisation of recombinant Humicola insolens endoglucanase produced in Pichia pastoris
Cellulases are industrially important hydrolytic enzymes that are applicable in the bioconversion of cellulosic biomass to simple sugars. In this work, Pichia pastoris carrying an endoglucanase cDNA (CMC3) from a thermophilic fungus, Humicola insolens, was grown in a 30 L bioreactor to produce rec...
| Main Authors: | Abdul Fattah, S.S., Mohamed, R., Wan Seman, W.M.K., Jahim, M.J., Illias, R.M., Abu Bakar, F.D., Murad, A.M.A. |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2015
|
| Online Access: | http://journalarticle.ukm.my/8723/ http://journalarticle.ukm.my/8723/1/44_1_25.pdf |
Similar Items
Cloning, heterologous expression and characterisation of a recombinant cellobiohydrolase from Humicola insolens ATCC16454 in Pichia pastoris
by: Amatul Samahah Md Ali,, et al.
Published: (2015)
by: Amatul Samahah Md Ali,, et al.
Published: (2015)
Overexpression, purification and characterization
of Aspergillus niger beta-glucosidase in Pichia pastoris
by: Kamaruddin, S., et al.
Published: (2015)
by: Kamaruddin, S., et al.
Published: (2015)
Characterization and potential applications of a
recombinant antifreeze protein from an antarctic
yeast Glaciozyma antarctica produced in Pichia pastoris
by: Md Tab M.,, et al.
Published: (2017)
by: Md Tab M.,, et al.
Published: (2017)
Production of long helical capsid of Nipah virus by Pichia pastoris.
by: Joseph, Narcisse M. S., et al.
Published: (2011)
by: Joseph, Narcisse M. S., et al.
Published: (2011)
Transformation of Chitinase in Yeast Pichia Pastoris GS115
by: Rasyidah, binti Ramlee
Published: (2015)
by: Rasyidah, binti Ramlee
Published: (2015)
Cloning of Aspergillus Niger BglA and expression of recombinant β-glucosidase in methylotrophic yeast Pichia Pastoris
by: Kamaruddin, Shazilah, et al.
Published: (2008)
by: Kamaruddin, Shazilah, et al.
Published: (2008)
Expression of segment a of infectious bursal disease virus in pichia pastoris
by: Ghazali, Mohd. Shahrin, et al.
Published: (2014)
by: Ghazali, Mohd. Shahrin, et al.
Published: (2014)
Cloning and expression of the Glomerella cingulata cutinase recombinant gene in the yeast, Pichia pastoris
by: Lias, Norhatiah Md., et al.
Published: (2004)
by: Lias, Norhatiah Md., et al.
Published: (2004)
Expression And Characterization Of Recombinant Thermostable L2 Lipase In Pichia Pastoris
by: Sabri, Suriana
Published: (2007)
by: Sabri, Suriana
Published: (2007)
Production of thermostable L2 lipase using recombinant Pichia pastoris
by: Shahidan, Noor Hidayah
Published: (2012)
by: Shahidan, Noor Hidayah
Published: (2012)
Expression of recombinant thermostable W200R protease in Pichia pastoris
by: Mohamad Sultan, Muhamad Zarir
Published: (2011)
by: Mohamad Sultan, Muhamad Zarir
Published: (2011)
Cloning And Expression Of Synthetic Human
Erythropoietin In Methylotrophic Yeast, Pichia Pastoris
by: Bustami, Yazmin
Published: (2010)
by: Bustami, Yazmin
Published: (2010)
Nucleocapsid and matrix proteins of nipah virus produced in Pichia pastoris and their interaction
by: Narcisse Mary, Sither Joseph Vesudian
Published: (2016)
by: Narcisse Mary, Sither Joseph Vesudian
Published: (2016)
Development and validation of promoter prediction system for recombinant lipase expression in Pichia pastoris
by: Gogo, Mayaki Fatima
Published: (2017)
by: Gogo, Mayaki Fatima
Published: (2017)
Expression of Alkaline Protease from Bacillus Stearothermophilus F1 in Pichia Pastoris
by: Ahmad Latiffi, Amaliawati
Published: (2008)
by: Ahmad Latiffi, Amaliawati
Published: (2008)
Cloning, expression and purification of squalene synthase from Candida tropicalis in Pichia pastoris
by: Lee, Pey Yee, et al.
Published: (2014)
by: Lee, Pey Yee, et al.
Published: (2014)
Expression of human epidermal growth factor in pichia pastoris and optimization of fermentation conditions
by: Eissazadeh, Samira
Published: (2012)
by: Eissazadeh, Samira
Published: (2012)
Expression of recombinant geobacillus SP a-amylase in pichia pastoris GS 115
by: Gandhi, Sivasangkary
Published: (2014)
by: Gandhi, Sivasangkary
Published: (2014)
Purification and characterization of recombinant thermostable T1 lipase expressed from Pichia pastoris
by: Periyasamy, Nalini
Published: (2015)
by: Periyasamy, Nalini
Published: (2015)
Biosynthetic production of human growth hormone gene in methylotrophic yeast, Pichia pastoris
by: Loh, Danley, et al.
Published: (2004)
by: Loh, Danley, et al.
Published: (2004)
Expression Of Recombinant Human Dna Topoisomerase In Pichia Pastoris And Analysis Of Its Activity
by: Chan, Mooi Kwai
Published: (2017)
by: Chan, Mooi Kwai
Published: (2017)
Native-state stability determines the extent of degradation relative to secretion of protein variants from Pichia pastoris
by: Whyteside, Graham, et al.
Published: (2011)
by: Whyteside, Graham, et al.
Published: (2011)
Production of the virus-like particles of nipah virus matrix protein in Pichia pastoris as diagnostic reagents
by: Joseph, Narcisse M. S., et al.
Published: (2016)
by: Joseph, Narcisse M. S., et al.
Published: (2016)
Molecular cloning of phytase gene from ASUIA279 and its expression in Pichia Pastoris system.
by: Shobirin, Anis, et al.
Published: (2011)
by: Shobirin, Anis, et al.
Published: (2011)
Humicola sp. as a cause of peritoneal dialysis-associated peritonitis
by: Burns, N., et al.
Published: (2015)
by: Burns, N., et al.
Published: (2015)
Molecular cloning of phytase gene from ASUIA279 and its expression in Pichia Pastoris system
by: Maifiah, M.H. Mahamad, et al.
Published: (2011)
by: Maifiah, M.H. Mahamad, et al.
Published: (2011)
Cloning and Expression of the Nucleocapsid Protein of Newcastle Disease Virus in Pichia Pastoris (Guillierm.) Phaff
by: Saidin, Syazwan
Published: (2010)
by: Saidin, Syazwan
Published: (2010)
The effect of carbon sources on the expression level of thermostable L2 lipase in Pichia pastoris
by: Shahidan, Noor Hidayah, et al.
Published: (2011)
by: Shahidan, Noor Hidayah, et al.
Published: (2011)
Pichia pastoris as a host to overexpress the thermostable T1 lipase from Geobacillus zalihae
by: Oslan, Siti Nurbaya, et al.
Published: (2014)
by: Oslan, Siti Nurbaya, et al.
Published: (2014)
Expression and characterization of Geobacillus stearothermophilus SR74 recombinant a-Amylase in Pichia pastoris
by: Gandhi, Sivasangkary, et al.
Published: (2015)
by: Gandhi, Sivasangkary, et al.
Published: (2015)
Cloning and expression of highly pathogenic avian influenza virus full-length nonstructural gene in Pichia pastoris.
by: M.B., Abu Bakar, et al.
Published: (2011)
by: M.B., Abu Bakar, et al.
Published: (2011)
Molecular cloning and production of recombinant phytase from Bacillus Subtilis ASUIA243 in Pichia Pastoris
by: Mohd Dali, Nor Soleha, et al.
Published: (2011)
by: Mohd Dali, Nor Soleha, et al.
Published: (2011)
Functional expression of Vanda Mimi Palmer monoterpene synthases in Pichia pastoris, a yeast system
by: Rahim Mohd-Hairul, Ab
Published: (2017)
by: Rahim Mohd-Hairul, Ab
Published: (2017)
Molecular cloning and production of recombinant phytase from bacillus subtilis ASUIA243 in Pichia Pastoris.
by: Mohd Dali , Nor Soleha, et al.
Published: (2011)
by: Mohd Dali , Nor Soleha, et al.
Published: (2011)
Feeding Strategies In Recombinant Human Erythropoietin Production
By Pichia Pastoris Fermentation
In Stirred-Tank Bioreactor
by: Shahkillahwati , Mohd Ridhwan
Published: (2014)
by: Shahkillahwati , Mohd Ridhwan
Published: (2014)
Development Of High Cell Density Recombinant Pichia Pastoris Culture For Human Growth Hormone Production
by: Lee, Yoeu Chek
Published: (2008)
by: Lee, Yoeu Chek
Published: (2008)
Characterisation of a recombinant β-xylosidase
(xylA) from Aspergillus oryzae expressed in Pichia
pastoris
by: Kirikyali, Narin, et al.
Published: (2014)
by: Kirikyali, Narin, et al.
Published: (2014)
Production of in vivo biotinylated scFv specific to almond (Prunus dulcis) proteins by recombinant Pichia pastoris
by: de la Cruz, Silvia, et al.
Published: (2016)
by: de la Cruz, Silvia, et al.
Published: (2016)
Heterologous, Expression, and Characterization of Thermostable Glucoamylase Derived from Aspergillus flavus NSH9 in Pichia pastoris
by: Kazi Muhammad, Rezaul Karim, et al.
Published: (2016)
by: Kazi Muhammad, Rezaul Karim, et al.
Published: (2016)
Heterologous, Expression, and Characterization of
Thermostable Glucoamylase Derived from Aspergillus flavus
NSH9 in Pichia pastoris
by: Kazi Muhammad, Rezaul Karim, et al.
Published: (2016)
by: Kazi Muhammad, Rezaul Karim, et al.
Published: (2016)
Similar Items
-
Cloning, heterologous expression and characterisation of a recombinant cellobiohydrolase from Humicola insolens ATCC16454 in Pichia pastoris
by: Amatul Samahah Md Ali,, et al.
Published: (2015) -
Overexpression, purification and characterization
of Aspergillus niger beta-glucosidase in Pichia pastoris
by: Kamaruddin, S., et al.
Published: (2015) -
Characterization and potential applications of a
recombinant antifreeze protein from an antarctic
yeast Glaciozyma antarctica produced in Pichia pastoris
by: Md Tab M.,, et al.
Published: (2017) -
Production of long helical capsid of Nipah virus by Pichia pastoris.
by: Joseph, Narcisse M. S., et al.
Published: (2011) -
Transformation of Chitinase in Yeast Pichia Pastoris GS115
by: Rasyidah, binti Ramlee
Published: (2015)