Overexpression, purification and characterization of Aspergillus niger beta-glucosidase in Pichia pastoris
This study describes the expression of β-glucosidase (BglA) from Aspergillus niger in Pichia pastoris, a methylotrophic yeast strain, under the regulation of an alcohol oxidase promoter. The heterologous expression of BglA was optimized in a shake flask. Optimal conditions were achieved using an i...
| Main Authors: | Kamaruddin, S., Abu Bakar, F.D., Illias, R.M., Said, M., Hassan, O., Murad, A.M.A. |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2015
|
| Online Access: | http://journalarticle.ukm.my/8690/ http://journalarticle.ukm.my/8690/1/44_1_02.pdf |
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