Triadimefon ameliorates negative effects of in vitro salt stress on two cultivars of alfalfa(Medicago sativa L.)

Triadimefon (TRD) is a member of triazol plant growth regulator group which is responsible for increasing stress tolerance. Effects of TRD on two cultivars of Medicago sativa (Hamedani and Yazdi) were investigated. The in vitro grown plantlets in MS medium were cultured in TRD at concentration 1.0,...

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Main Authors: Leila, A., Ehsanpour, A.
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2013
Online Access:http://journalarticle.ukm.my/7201/
http://journalarticle.ukm.my/7201/1/42_1_02.pdf
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author Leila, A.
Ehsanpour, A.
author_facet Leila, A.
Ehsanpour, A.
author_sort Leila, A.
building UKM Institutional Repository
collection Online Access
description Triadimefon (TRD) is a member of triazol plant growth regulator group which is responsible for increasing stress tolerance. Effects of TRD on two cultivars of Medicago sativa (Hamedani and Yazdi) were investigated. The in vitro grown plantlets in MS medium were cultured in TRD at concentration 1.0, 2.0 and 4.0 mg/l combined with 0, 100 and 140 mM NaCl respectively. The changes in growth parameters, photosynthetic pigment, carbohydrate, α-tochopherol, glycine betain, proline and proline dehydrogenase (PDH) activity as well as its expression were measured after four weeks post treatment. NaCl treatment decreased overall plant growth and reduced the total chlorophyll, carbohydrate content, PDH activity and increased α-tochopherol, glycine betain content and proline content. The level of PDH transcript was changed in different combinations of NaCl and TRD, however, at 140 mM NaCl and 2 mg/l TRD expression was decreased. When plants were subjected to salt stress, TRD minimized the inhibitory effects of NaCl on plant growth by increasing, total chlorophyll, carbohydrate, α-tochopherol, glycine betaine content.
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spelling oai:generic.eprints.org:72012016-12-14T06:43:23Z http://journalarticle.ukm.my/7201/ Triadimefon ameliorates negative effects of in vitro salt stress on two cultivars of alfalfa(Medicago sativa L.) Leila, A. Ehsanpour, A. Triadimefon (TRD) is a member of triazol plant growth regulator group which is responsible for increasing stress tolerance. Effects of TRD on two cultivars of Medicago sativa (Hamedani and Yazdi) were investigated. The in vitro grown plantlets in MS medium were cultured in TRD at concentration 1.0, 2.0 and 4.0 mg/l combined with 0, 100 and 140 mM NaCl respectively. The changes in growth parameters, photosynthetic pigment, carbohydrate, α-tochopherol, glycine betain, proline and proline dehydrogenase (PDH) activity as well as its expression were measured after four weeks post treatment. NaCl treatment decreased overall plant growth and reduced the total chlorophyll, carbohydrate content, PDH activity and increased α-tochopherol, glycine betain content and proline content. The level of PDH transcript was changed in different combinations of NaCl and TRD, however, at 140 mM NaCl and 2 mg/l TRD expression was decreased. When plants were subjected to salt stress, TRD minimized the inhibitory effects of NaCl on plant growth by increasing, total chlorophyll, carbohydrate, α-tochopherol, glycine betaine content. Penerbit Universiti Kebangsaan Malaysia 2013-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7201/1/42_1_02.pdf Leila, A. and Ehsanpour, A. (2013) Triadimefon ameliorates negative effects of in vitro salt stress on two cultivars of alfalfa(Medicago sativa L.). Malaysian Applied Biology, 42 (1). pp. 15-24. ISSN 0126-8643 http://mabjournal.com/
spellingShingle Leila, A.
Ehsanpour, A.
Triadimefon ameliorates negative effects of in vitro salt stress on two cultivars of alfalfa(Medicago sativa L.)
title Triadimefon ameliorates negative effects of in vitro salt stress on two cultivars of alfalfa(Medicago sativa L.)
title_full Triadimefon ameliorates negative effects of in vitro salt stress on two cultivars of alfalfa(Medicago sativa L.)
title_fullStr Triadimefon ameliorates negative effects of in vitro salt stress on two cultivars of alfalfa(Medicago sativa L.)
title_full_unstemmed Triadimefon ameliorates negative effects of in vitro salt stress on two cultivars of alfalfa(Medicago sativa L.)
title_short Triadimefon ameliorates negative effects of in vitro salt stress on two cultivars of alfalfa(Medicago sativa L.)
title_sort triadimefon ameliorates negative effects of in vitro salt stress on two cultivars of alfalfa(medicago sativa l.)
url http://journalarticle.ukm.my/7201/
http://journalarticle.ukm.my/7201/
http://journalarticle.ukm.my/7201/1/42_1_02.pdf