Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids
A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis,cis-11,12;14,15-diepoxyeicosanoate) were isolated from the matrix by transeste...
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2014
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| Online Access: | http://journalarticle.ukm.my/6926/ http://journalarticle.ukm.my/6926/1/10_Hong_Keat_Quek.pdf |
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| author | Hong, Keat Quek Mohd Marsin Sanagi, Wan Aini Wan Ibrahim, Syairah Salleh, Mazidatulakmam Miskam, |
| author_facet | Hong, Keat Quek Mohd Marsin Sanagi, Wan Aini Wan Ibrahim, Syairah Salleh, Mazidatulakmam Miskam, |
| author_sort | Hong, Keat Quek |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis,cis-11,12;14,15-diepoxyeicosanoate) were isolated from the matrix by transesterification process. The developed method showed good linear dynamic range between 0.7-20 μg mL-1 with coefficients of determination (R2) > 0.9968 and acceptable limit of detection and limit of quantitation of 7 and 23 mg kg-1, respectively, based on linearity calculations. Analyte recoveries were 90.84±27.24% for low concentration, 78.05±11.59% for medium concentration and 99.23±10.20% for high concentration. This first fully validated GC-MS method was successfully applied for the determination of ESBO in foods stored in glass jar with metal lid. Among the 31 food samples studied, 6 samples were found to exceed the specific migration limit of 60 mg kg-1 (based on EU Directive 2002/72/EC). The developed method is thus potentially useful for routine analysis for the determination of ESBO. |
| first_indexed | 2025-11-14T23:44:24Z |
| format | Article |
| id | oai:generic.eprints.org:6926 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:44:24Z |
| publishDate | 2014 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
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| spelling | oai:generic.eprints.org:69262016-12-14T06:42:36Z http://journalarticle.ukm.my/6926/ Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids Hong, Keat Quek Mohd Marsin Sanagi, Wan Aini Wan Ibrahim, Syairah Salleh, Mazidatulakmam Miskam, A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis,cis-11,12;14,15-diepoxyeicosanoate) were isolated from the matrix by transesterification process. The developed method showed good linear dynamic range between 0.7-20 μg mL-1 with coefficients of determination (R2) > 0.9968 and acceptable limit of detection and limit of quantitation of 7 and 23 mg kg-1, respectively, based on linearity calculations. Analyte recoveries were 90.84±27.24% for low concentration, 78.05±11.59% for medium concentration and 99.23±10.20% for high concentration. This first fully validated GC-MS method was successfully applied for the determination of ESBO in foods stored in glass jar with metal lid. Among the 31 food samples studied, 6 samples were found to exceed the specific migration limit of 60 mg kg-1 (based on EU Directive 2002/72/EC). The developed method is thus potentially useful for routine analysis for the determination of ESBO. Universiti Kebangsaan Malaysia 2014-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6926/1/10_Hong_Keat_Quek.pdf Hong, Keat Quek and Mohd Marsin Sanagi, and Wan Aini Wan Ibrahim, and Syairah Salleh, and Mazidatulakmam Miskam, (2014) Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids. Sains Malaysiana, 43 (3). pp. 407-413. ISSN 0126-6039 http://www.ukm.my/jsm/ |
| spellingShingle | Hong, Keat Quek Mohd Marsin Sanagi, Wan Aini Wan Ibrahim, Syairah Salleh, Mazidatulakmam Miskam, Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids |
| title | Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids |
| title_full | Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids |
| title_fullStr | Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids |
| title_full_unstemmed | Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids |
| title_short | Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids |
| title_sort | development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids |
| url | http://journalarticle.ukm.my/6926/ http://journalarticle.ukm.my/6926/ http://journalarticle.ukm.my/6926/1/10_Hong_Keat_Quek.pdf |