Antioxidant activity in crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables

In this study, crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables namely, garlic chives (Allium tuberosum), celery (Apium graveolens (L.)), sweet potato leaves (Ipomoea batatas (L.)), curry leaves (Murraya koenigii (L.)), winged beans (Psophocarpus tetragonol...

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Main Authors: Sumathi Murti, Nurhayati Zainal Abidin, Ashril Yusof
Format: Article
Language:English
Published: Universiti Kebangsaan Malaysia 2013
Online Access:http://journalarticle.ukm.my/6467/
http://journalarticle.ukm.my/6467/1/07_Sumathi_Murti.pdf
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author Sumathi Murti,
Nurhayati Zainal Abidin,
Ashril Yusof,
author_facet Sumathi Murti,
Nurhayati Zainal Abidin,
Ashril Yusof,
author_sort Sumathi Murti,
building UKM Institutional Repository
collection Online Access
description In this study, crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables namely, garlic chives (Allium tuberosum), celery (Apium graveolens (L.)), sweet potato leaves (Ipomoea batatas (L.)), curry leaves (Murraya koenigii (L.)), winged beans (Psophocarpus tetragonolobus) and sweet leaves (Sauropus androgynus) were tested for antioxidant activities using three bioassays namely 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, reducing power assay and metal chelating assay. Overall, among the 24 crude extracts tested, petroleum benzene and chloroform extract of Murraya koenigii (L.) showed higher free radical scavenging activities (IC50 = 0.02 mg/mL and 0.0225 mg/mL, respectively) when compared to ascorbic acid (IC50 = 0.00375 mg/mL) and strong reducing powers with absorbance value of 1.208 ± 0.006 and 1.833 ± 0.003 when compared to butylated hydroxyanisole with absorbance value of 2.625 ± 0.004 at the highest concentration tested, 1 mg/mL in both DPPH and reducing power assay, respectively. In metal chelating assay, methanol, petroleum benzene and chloroform extracts of Murraya koenigii (L.) showed moderate metal chelating activities of 88.60 ± 0.02%, 78.30 ± 0.23% and 73.61 ± 0.22%, respectively, at 1 mg/mL when compared to ethylenediaminetetraacetic acid which is 98.63 ± 0.13%. These findings suggested the important significance of Murraya koenigii (L.) consumption in prevention of diseases.
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spelling oai:generic.eprints.org:64672016-12-14T06:41:16Z http://journalarticle.ukm.my/6467/ Antioxidant activity in crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables Sumathi Murti, Nurhayati Zainal Abidin, Ashril Yusof, In this study, crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables namely, garlic chives (Allium tuberosum), celery (Apium graveolens (L.)), sweet potato leaves (Ipomoea batatas (L.)), curry leaves (Murraya koenigii (L.)), winged beans (Psophocarpus tetragonolobus) and sweet leaves (Sauropus androgynus) were tested for antioxidant activities using three bioassays namely 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, reducing power assay and metal chelating assay. Overall, among the 24 crude extracts tested, petroleum benzene and chloroform extract of Murraya koenigii (L.) showed higher free radical scavenging activities (IC50 = 0.02 mg/mL and 0.0225 mg/mL, respectively) when compared to ascorbic acid (IC50 = 0.00375 mg/mL) and strong reducing powers with absorbance value of 1.208 ± 0.006 and 1.833 ± 0.003 when compared to butylated hydroxyanisole with absorbance value of 2.625 ± 0.004 at the highest concentration tested, 1 mg/mL in both DPPH and reducing power assay, respectively. In metal chelating assay, methanol, petroleum benzene and chloroform extracts of Murraya koenigii (L.) showed moderate metal chelating activities of 88.60 ± 0.02%, 78.30 ± 0.23% and 73.61 ± 0.22%, respectively, at 1 mg/mL when compared to ethylenediaminetetraacetic acid which is 98.63 ± 0.13%. These findings suggested the important significance of Murraya koenigii (L.) consumption in prevention of diseases. Universiti Kebangsaan Malaysia 2013-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/6467/1/07_Sumathi_Murti.pdf Sumathi Murti, and Nurhayati Zainal Abidin, and Ashril Yusof, (2013) Antioxidant activity in crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables. Sains Malaysiana, 42 (9). pp. 1253-1259. ISSN 0126-6039 http://www.ukm.my/jsm/
spellingShingle Sumathi Murti,
Nurhayati Zainal Abidin,
Ashril Yusof,
Antioxidant activity in crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables
title Antioxidant activity in crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables
title_full Antioxidant activity in crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables
title_fullStr Antioxidant activity in crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables
title_full_unstemmed Antioxidant activity in crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables
title_short Antioxidant activity in crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables
title_sort antioxidant activity in crude petroleum benzene, chloroform, methanol and water extracts of six selected vegetables
url http://journalarticle.ukm.my/6467/
http://journalarticle.ukm.my/6467/
http://journalarticle.ukm.my/6467/1/07_Sumathi_Murti.pdf