Limit of DNA Barcode in delineating penaeus monodon and in its developing stages
Mysis, post larvae and adult specimens of Penaeus monodon were sequenced for Cyctochrome C Oxidase subunit I (COI) gene (DNA barcode) to check the efficiency of DNA barcode in delineating species irrespective of its different life stages. COI gene sequences of different life stages of P. monodon wer...
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2012
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| Online Access: | http://journalarticle.ukm.my/5669/ http://journalarticle.ukm.my/5669/1/05%2520C.%2520Prasanna%2520Kumar.pdf |
| _version_ | 1848810904002494464 |
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| author | Prasanna Kumar, C Akbar John, B Ajmal Khan, S Lyla, P.S Jalal, K.C.A |
| author_facet | Prasanna Kumar, C Akbar John, B Ajmal Khan, S Lyla, P.S Jalal, K.C.A |
| author_sort | Prasanna Kumar, C |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Mysis, post larvae and adult specimens of Penaeus monodon were sequenced for Cyctochrome C Oxidase subunit I (COI) gene (DNA barcode) to check the efficiency of DNA barcode in delineating species irrespective of its different life stages. COI gene sequences of different life stages of P. monodon were subjected to pair-wise distance analysis, nucleotide sequence diversity estimation, phylogenetic and BOLD analysis. The pair-wise distance estimation revealed that intra-specific variations within the barcode sequences of P. monodon were low when compared with other species of genus Penaeus spp. The average pair-wise distance within the sequences of different stages of P. monodon was two times lesser than the pair-wise distance of the out-group used. The nucleotide diversity within the barcodes were low (π = 0.324). The constructed phylogram clearly showed that the DNA barcoding using partial COI gene would provide an accurate delineation of species irrespective of their different life stages. The BOLD analysis accurately identified all sequences as belonging to P. monodon. |
| first_indexed | 2025-11-14T23:37:54Z |
| format | Article |
| id | oai:generic.eprints.org:5669 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:37:54Z |
| publishDate | 2012 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
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| spelling | oai:generic.eprints.org:56692016-12-14T06:39:08Z http://journalarticle.ukm.my/5669/ Limit of DNA Barcode in delineating penaeus monodon and in its developing stages Prasanna Kumar, C Akbar John, B Ajmal Khan, S Lyla, P.S Jalal, K.C.A Mysis, post larvae and adult specimens of Penaeus monodon were sequenced for Cyctochrome C Oxidase subunit I (COI) gene (DNA barcode) to check the efficiency of DNA barcode in delineating species irrespective of its different life stages. COI gene sequences of different life stages of P. monodon were subjected to pair-wise distance analysis, nucleotide sequence diversity estimation, phylogenetic and BOLD analysis. The pair-wise distance estimation revealed that intra-specific variations within the barcode sequences of P. monodon were low when compared with other species of genus Penaeus spp. The average pair-wise distance within the sequences of different stages of P. monodon was two times lesser than the pair-wise distance of the out-group used. The nucleotide diversity within the barcodes were low (π = 0.324). The constructed phylogram clearly showed that the DNA barcoding using partial COI gene would provide an accurate delineation of species irrespective of their different life stages. The BOLD analysis accurately identified all sequences as belonging to P. monodon. Universiti Kebangsaan Malaysia 2012-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/5669/1/05%2520C.%2520Prasanna%2520Kumar.pdf Prasanna Kumar, C and Akbar John, B and Ajmal Khan, S and Lyla, P.S and Jalal, K.C.A (2012) Limit of DNA Barcode in delineating penaeus monodon and in its developing stages. Sains Malaysiana, 41 (12). pp. 1527-1533. ISSN 0126-6039 http://www.ukm.my/jsm/ |
| spellingShingle | Prasanna Kumar, C Akbar John, B Ajmal Khan, S Lyla, P.S Jalal, K.C.A Limit of DNA Barcode in delineating penaeus monodon and in its developing stages |
| title | Limit of DNA Barcode in delineating penaeus monodon and in its developing stages |
| title_full | Limit of DNA Barcode in delineating penaeus monodon and in its developing stages |
| title_fullStr | Limit of DNA Barcode in delineating penaeus monodon and in its developing stages |
| title_full_unstemmed | Limit of DNA Barcode in delineating penaeus monodon and in its developing stages |
| title_short | Limit of DNA Barcode in delineating penaeus monodon and in its developing stages |
| title_sort | limit of dna barcode in delineating penaeus monodon and in its developing stages |
| url | http://journalarticle.ukm.my/5669/ http://journalarticle.ukm.my/5669/ http://journalarticle.ukm.my/5669/1/05%2520C.%2520Prasanna%2520Kumar.pdf |