In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine
Acanthamoeba keratitis is a serious infection of the eye which can result in permanent visual impairment. Therefore this study was performed to evaluate the effectiveness of antimicrobial agents on three Acanthamoeba clinical isolates (HS 6, HKL 95 and HTH 73). Antimicrobial agents used in this stud...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2012
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| Online Access: | http://journalarticle.ukm.my/4469/ http://journalarticle.ukm.my/4469/1/07%2520Noradilah.pdf |
| _version_ | 1848810528530497536 |
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| author | Noradilah Samseh Abdullah, Mohamed Kamel Abd Ghani, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad, |
| author_facet | Noradilah Samseh Abdullah, Mohamed Kamel Abd Ghani, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad, |
| author_sort | Noradilah Samseh Abdullah, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Acanthamoeba keratitis is a serious infection of the eye which can result in permanent visual impairment. Therefore this study was performed to evaluate the effectiveness of antimicrobial agents on three Acanthamoeba clinical isolates (HS 6, HKL 95 and HTH 73). Antimicrobial agents used in this study were polyhexamethylene biguanide (PHMB) and chlorhexidine and both were serially diluted. Cyst suspensions from all three strains were tested against the antimicrobial agents, respectively. After 48 h of incubation at 37°C, the suspension was filtered and the filter membrane was placed onto non-nutrient agar plate lawned with heat-killed Escherichia coli. The plates were examined daily under the inverted microscope until day 14 but were negative for Acanthamoeba trophozoites. The presence of trophozoites indicated ineffectiveness of the antimicrobial agents. Both antimicrobial agents used were found to be effective against Acanthamoeba cysts for all the strains tested. PHMB gave minimum cysticidal concentration (MCC) mean value of 4.232 μg/mL and chlorhexidine showed MCC mean value of 3.906 μg/mL. So, from this study, it can be concluded that PHMB and chlorhexidine were effective in killing the tested Acanthamoeba cysts |
| first_indexed | 2025-11-14T23:31:56Z |
| format | Article |
| id | oai:generic.eprints.org:4469 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:31:56Z |
| publishDate | 2012 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:44692016-12-14T06:36:06Z http://journalarticle.ukm.my/4469/ In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine Noradilah Samseh Abdullah, Mohamed Kamel Abd Ghani, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad, Acanthamoeba keratitis is a serious infection of the eye which can result in permanent visual impairment. Therefore this study was performed to evaluate the effectiveness of antimicrobial agents on three Acanthamoeba clinical isolates (HS 6, HKL 95 and HTH 73). Antimicrobial agents used in this study were polyhexamethylene biguanide (PHMB) and chlorhexidine and both were serially diluted. Cyst suspensions from all three strains were tested against the antimicrobial agents, respectively. After 48 h of incubation at 37°C, the suspension was filtered and the filter membrane was placed onto non-nutrient agar plate lawned with heat-killed Escherichia coli. The plates were examined daily under the inverted microscope until day 14 but were negative for Acanthamoeba trophozoites. The presence of trophozoites indicated ineffectiveness of the antimicrobial agents. Both antimicrobial agents used were found to be effective against Acanthamoeba cysts for all the strains tested. PHMB gave minimum cysticidal concentration (MCC) mean value of 4.232 μg/mL and chlorhexidine showed MCC mean value of 3.906 μg/mL. So, from this study, it can be concluded that PHMB and chlorhexidine were effective in killing the tested Acanthamoeba cysts Universiti Kebangsaan Malaysia 2012-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/4469/1/07%2520Noradilah.pdf Noradilah Samseh Abdullah, and Mohamed Kamel Abd Ghani, and Anisah Nordin, and Yusof Suboh, and Noraina Ab Rahim, and Norazah Ahmad, (2012) In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine. Sains Malaysiana, 41 (5). pp. 569-572. ISSN 0126-6039 http://www.ukm.my/jsm |
| spellingShingle | Noradilah Samseh Abdullah, Mohamed Kamel Abd Ghani, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad, In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine |
| title | In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine |
| title_full | In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine |
| title_fullStr | In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine |
| title_full_unstemmed | In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine |
| title_short | In vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (PHMB) and chlorhexidine |
| title_sort | in vitro sensitivity testing of acanthamoeba clinical isolates from patients with keratitis against polyhexamethylene biguanide (phmb) and chlorhexidine |
| url | http://journalarticle.ukm.my/4469/ http://journalarticle.ukm.my/4469/ http://journalarticle.ukm.my/4469/1/07%2520Noradilah.pdf |