Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l
We report here the isolation and sequence analysis of a cDNA fragment for an endoglucanase gene from Aspergillus terreus SUK-I. A pair of primers was designed based on conserved regions of endoglucanase gene. Amplification of A. terreus SUK-I cDNA using the primers produced a DNA band of 642 bp. The...
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Universiti Kebangsaan Malaysia
2006
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| Online Access: | http://journalarticle.ukm.my/3971/ |
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| author | Nik Marzuki Sidik, Shaiful Adzni Sharifuddin, Othman Omar , Abdul Jalil Abdul Kader, Sahidan Senafi, |
| author_facet | Nik Marzuki Sidik, Shaiful Adzni Sharifuddin, Othman Omar , Abdul Jalil Abdul Kader, Sahidan Senafi, |
| author_sort | Nik Marzuki Sidik, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | We report here the isolation and sequence analysis of a cDNA fragment for an endoglucanase gene from Aspergillus terreus SUK-I. A pair of primers was designed based on conserved regions of endoglucanase gene. Amplification of A. terreus SUK-I cDNA using the primers produced a DNA band of 642 bp. The cDNA fragment was purified and cloned into pCR®ll-TOPO plasmid vector. The cDNA insert was designated as SA2 and sequenced. Analysis of the SA2 sequence showed a high degree of identity towards endoglucanase gene sequences from A. aculeatus, A kawachii and Talaromyces emersonii. While, the putative amino acid sequence of SA2 showed 74 % identity towards endoglucanase protein from Thermoascus aurantiacus and 69 % identity towards endoglucanase protein from A. kawachii, A. niger, A. aculeatus and A. nidulans. A 66 % identity between SA2 putative amino acid sequence and A. oryzae endoglucanase protein sequence was also observed. Therefore, we concluded that SA2 codes for a putative endoglucanase gene of A. terreus SUK-I. |
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| format | Article |
| id | oai:generic.eprints.org:3971 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| last_indexed | 2025-11-14T23:29:22Z |
| publishDate | 2006 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
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| spelling | oai:generic.eprints.org:39712012-03-28T00:53:11Z http://journalarticle.ukm.my/3971/ Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l Nik Marzuki Sidik, Shaiful Adzni Sharifuddin, Othman Omar , Abdul Jalil Abdul Kader, Sahidan Senafi, We report here the isolation and sequence analysis of a cDNA fragment for an endoglucanase gene from Aspergillus terreus SUK-I. A pair of primers was designed based on conserved regions of endoglucanase gene. Amplification of A. terreus SUK-I cDNA using the primers produced a DNA band of 642 bp. The cDNA fragment was purified and cloned into pCR®ll-TOPO plasmid vector. The cDNA insert was designated as SA2 and sequenced. Analysis of the SA2 sequence showed a high degree of identity towards endoglucanase gene sequences from A. aculeatus, A kawachii and Talaromyces emersonii. While, the putative amino acid sequence of SA2 showed 74 % identity towards endoglucanase protein from Thermoascus aurantiacus and 69 % identity towards endoglucanase protein from A. kawachii, A. niger, A. aculeatus and A. nidulans. A 66 % identity between SA2 putative amino acid sequence and A. oryzae endoglucanase protein sequence was also observed. Therefore, we concluded that SA2 codes for a putative endoglucanase gene of A. terreus SUK-I. Universiti Kebangsaan Malaysia 2006-07 Article PeerReviewed Nik Marzuki Sidik, and Shaiful Adzni Sharifuddin, and Othman Omar , and Abdul Jalil Abdul Kader, and Sahidan Senafi, (2006) Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l. Sains Malaysiana, 35 (1). pp. 31-35. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol35num1_2006/vol35num1_06page31-35.html |
| spellingShingle | Nik Marzuki Sidik, Shaiful Adzni Sharifuddin, Othman Omar , Abdul Jalil Abdul Kader, Sahidan Senafi, Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l |
| title | Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l |
| title_full | Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l |
| title_fullStr | Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l |
| title_full_unstemmed | Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l |
| title_short | Isolation and molecular analysis of cDNA encoding a partial endoglucanase gene from aspergillus terreus SUK-l |
| title_sort | isolation and molecular analysis of cdna encoding a partial endoglucanase gene from aspergillus terreus suk-l |
| url | http://journalarticle.ukm.my/3971/ http://journalarticle.ukm.my/3971/ |