Challenges in culturing macaca fascicularisBone marrow stem cells
Culturing Macaca fascicularis bone marrow stem cells in fetal bovine serum (FBS) resulted in low proliferation and long period of incubation. Therefore, its potential uses are exhausted. Here we report the establishment of culturing the Macaca fascicularis bone marrow stem cells using the FBS in com...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universiti Kebangsaan Malaysia
2012
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| Online Access: | http://journalarticle.ukm.my/3930/ http://journalarticle.ukm.my/3930/1/05%2520Sharen.pdf |
| _version_ | 1848810350332346368 |
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| author | Sharen, S@Sharen Aini Masfueh, R Badiah, B Ng, M.H Shamsul, B.S Chua, K.H Low, C.K Mohd Nazimi, A.J Rifqah, N Norziha, Y Shariffah Shuriana, A.S Ruszymah, B.H.I Siar, C Ramli, R |
| author_facet | Sharen, S@Sharen Aini Masfueh, R Badiah, B Ng, M.H Shamsul, B.S Chua, K.H Low, C.K Mohd Nazimi, A.J Rifqah, N Norziha, Y Shariffah Shuriana, A.S Ruszymah, B.H.I Siar, C Ramli, R |
| author_sort | Sharen, S@Sharen Aini |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Culturing Macaca fascicularis bone marrow stem cells in fetal bovine serum (FBS) resulted in low proliferation and long period of incubation. Therefore, its potential uses are exhausted. Here we report the establishment of culturing the Macaca fascicularis bone marrow stem cells using the FBS in combination with autologous serum. Five percent autologous serum was added to the Minimum Essential Medium (MEM) alpha medium and 10% FBS while 0.2 mM acid ascorbic 2-phosphate, 10 mM β-glycerolphosphate, 10-8 molar dexamethasone were used for osteogenic induction. Following this combination, our results showed higher growth kinetic i.e. 1.41% growth rate higher compared to only 0.46% growth rates of the cells using FBS alone and shorter population doubling time (4 to 7 days) compared to the culture without the combination of FBS and autologous serum (30 days). Thus, the combination of the FBS and autologous serum permits fast cell growth and tissue construction. |
| first_indexed | 2025-11-14T23:29:06Z |
| format | Article |
| id | oai:generic.eprints.org:3930 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:29:06Z |
| publishDate | 2012 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:39302016-12-14T06:35:20Z http://journalarticle.ukm.my/3930/ Challenges in culturing macaca fascicularisBone marrow stem cells Sharen, S@Sharen Aini Masfueh, R Badiah, B Ng, M.H Shamsul, B.S Chua, K.H Low, C.K Mohd Nazimi, A.J Rifqah, N Norziha, Y Shariffah Shuriana, A.S Ruszymah, B.H.I Siar, C Ramli, R Culturing Macaca fascicularis bone marrow stem cells in fetal bovine serum (FBS) resulted in low proliferation and long period of incubation. Therefore, its potential uses are exhausted. Here we report the establishment of culturing the Macaca fascicularis bone marrow stem cells using the FBS in combination with autologous serum. Five percent autologous serum was added to the Minimum Essential Medium (MEM) alpha medium and 10% FBS while 0.2 mM acid ascorbic 2-phosphate, 10 mM β-glycerolphosphate, 10-8 molar dexamethasone were used for osteogenic induction. Following this combination, our results showed higher growth kinetic i.e. 1.41% growth rate higher compared to only 0.46% growth rates of the cells using FBS alone and shorter population doubling time (4 to 7 days) compared to the culture without the combination of FBS and autologous serum (30 days). Thus, the combination of the FBS and autologous serum permits fast cell growth and tissue construction. Universiti Kebangsaan Malaysia 2012-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/3930/1/05%2520Sharen.pdf Sharen, S@Sharen Aini and Masfueh, R and Badiah, B and Ng, M.H and Shamsul, B.S and Chua, K.H and Low, C.K and Mohd Nazimi, A.J and Rifqah, N and Norziha, Y and Shariffah Shuriana, A.S and Ruszymah, B.H.I and Siar, C and Ramli, R (2012) Challenges in culturing macaca fascicularisBone marrow stem cells. Sains Malaysiana, 41 (4). pp. 419-422. ISSN 0126-6039 http://www.ukm.my/jsm/ |
| spellingShingle | Sharen, S@Sharen Aini Masfueh, R Badiah, B Ng, M.H Shamsul, B.S Chua, K.H Low, C.K Mohd Nazimi, A.J Rifqah, N Norziha, Y Shariffah Shuriana, A.S Ruszymah, B.H.I Siar, C Ramli, R Challenges in culturing macaca fascicularisBone marrow stem cells |
| title | Challenges in culturing macaca fascicularisBone marrow stem cells |
| title_full | Challenges in culturing macaca fascicularisBone marrow stem cells |
| title_fullStr | Challenges in culturing macaca fascicularisBone marrow stem cells |
| title_full_unstemmed | Challenges in culturing macaca fascicularisBone marrow stem cells |
| title_short | Challenges in culturing macaca fascicularisBone marrow stem cells |
| title_sort | challenges in culturing macaca fascicularisbone marrow stem cells |
| url | http://journalarticle.ukm.my/3930/ http://journalarticle.ukm.my/3930/ http://journalarticle.ukm.my/3930/1/05%2520Sharen.pdf |