Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts
Replicative senescence of human diploid fibroblasts (HDFs) occurs when cells lose their capacity to proliferate and enter a phase of irreversible growth arrest. Stress-induced premature senescence (SIPS) on the other hand is caused by subcytotoxic concentrations of various oxidants which trigger acc...
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2011
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| Online Access: | http://journalarticle.ukm.my/2935/ http://journalarticle.ukm.my/2935/1/07_Haryati.pdf |
| _version_ | 1848810022426902528 |
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| author | Goon Jo Aan, Haryati Ahmad Hairi, Suzana Makpol, Mariati Abdul Rahman, Saiful Anuar Karsani, Wan Zurinah Wan Ngah, |
| author_facet | Goon Jo Aan, Haryati Ahmad Hairi, Suzana Makpol, Mariati Abdul Rahman, Saiful Anuar Karsani, Wan Zurinah Wan Ngah, |
| author_sort | Goon Jo Aan, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Replicative senescence of human diploid fibroblasts (HDFs) occurs when cells lose their capacity to proliferate and enter a phase of irreversible growth arrest. Stress-induced premature senescence (SIPS) on the other hand is caused by subcytotoxic concentrations of various oxidants which trigger accelerated cellular senescence. In this study, a SIPS model was established by exposing human diploid fibroblasts (HDFs) to 20 μM H2O2 for 2 weeks. A proteomic comparison between young, senescent and SIPS cells was done using two dimensional gel electrophoresis (2DGE) to elucidate the changes in protein expression associated with cellular aging. Our analysis showed that 28 protein spots were differentially expressed in senescent cells whereas 10 protein spots were differentially expressed in SIPS as compared to young cells. Three similar protein spots were differentially expressed in both senescent and SIPS cells when compared to the young cells. These results indicate that a difference in protein expression exists between senescent cells and SIPS cells compared to young cells. |
| first_indexed | 2025-11-14T23:23:54Z |
| format | Article |
| id | oai:generic.eprints.org:2935 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:23:54Z |
| publishDate | 2011 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
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| spelling | oai:generic.eprints.org:29352016-12-14T06:33:06Z http://journalarticle.ukm.my/2935/ Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts Goon Jo Aan, Haryati Ahmad Hairi, Suzana Makpol, Mariati Abdul Rahman, Saiful Anuar Karsani, Wan Zurinah Wan Ngah, Replicative senescence of human diploid fibroblasts (HDFs) occurs when cells lose their capacity to proliferate and enter a phase of irreversible growth arrest. Stress-induced premature senescence (SIPS) on the other hand is caused by subcytotoxic concentrations of various oxidants which trigger accelerated cellular senescence. In this study, a SIPS model was established by exposing human diploid fibroblasts (HDFs) to 20 μM H2O2 for 2 weeks. A proteomic comparison between young, senescent and SIPS cells was done using two dimensional gel electrophoresis (2DGE) to elucidate the changes in protein expression associated with cellular aging. Our analysis showed that 28 protein spots were differentially expressed in senescent cells whereas 10 protein spots were differentially expressed in SIPS as compared to young cells. Three similar protein spots were differentially expressed in both senescent and SIPS cells when compared to the young cells. These results indicate that a difference in protein expression exists between senescent cells and SIPS cells compared to young cells. Universiti Kebangsaan Malaysia 2011-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2935/1/07_Haryati.pdf Goon Jo Aan, and Haryati Ahmad Hairi, and Suzana Makpol, and Mariati Abdul Rahman, and Saiful Anuar Karsani, and Wan Zurinah Wan Ngah, (2011) Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts. Sains Malaysiana, 40 (11). pp. 1247-1253. ISSN 0126-6039 http://www.ukm.my/jsm/contents.html |
| spellingShingle | Goon Jo Aan, Haryati Ahmad Hairi, Suzana Makpol, Mariati Abdul Rahman, Saiful Anuar Karsani, Wan Zurinah Wan Ngah, Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts |
| title | Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts |
| title_full | Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts |
| title_fullStr | Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts |
| title_full_unstemmed | Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts |
| title_short | Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts |
| title_sort | differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (sips) fibroblasts |
| url | http://journalarticle.ukm.my/2935/ http://journalarticle.ukm.my/2935/ http://journalarticle.ukm.my/2935/1/07_Haryati.pdf |