Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process
B. subtilis under certain types of media and fermentation conditions can produce surfactin, a biosurfactant which belongs to the lipopeptide class. Surfactin has exceptional surfactant activity, and exhibits some interesting biological characteristics such as antibacterial activity, antitumoral acti...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2011
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| Online Access: | http://journalarticle.ukm.my/2639/ http://journalarticle.ukm.my/2639/1/15_Mohd_Hafez.pdf |
| _version_ | 1848809929244147712 |
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| author | Mohd. Hafez Mohd Isa, Richard Frazier .A, Paula Jauregi, |
| author_facet | Mohd. Hafez Mohd Isa, Richard Frazier .A, Paula Jauregi, |
| author_sort | Mohd. Hafez Mohd Isa, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | B. subtilis under certain types of media and fermentation conditions can produce surfactin, a biosurfactant which belongs to the lipopeptide class. Surfactin has exceptional surfactant activity, and exhibits some interesting biological characteristics such as antibacterial activity, antitumoral activity against ascites carcinoma cells, and a hypocholesterolemic activity that inhibits cAMP phosphodiesterase, as well as having anti-HIV properties. A cost effective recovery and purification of surfactin from fermentation broth using a two-step ultrafiltration (UF) process has been developed in order to reduce the cost of surfactin production. In this study, competitive adsorption of surfactin and proteins at the air-water interface was studied using surface pressure measurements. Small volumes of bovine serum albumin (BSA) and β-casein solutions were added to the air-water interface on a Langmuir trough and allowed to stabilise before the addition of surfactin to the subphase. Contrasting interfacial behaviour of proteins was observed with β-casein showing faster initial adsorption compared to BSA. On introduction of surfactin both proteins were displaced but a longer time were taken to displace β-casein. Overall the results showed surfactin were highly surface-active by forming a β-sheet structure at the air-water interface after reaching its critical micelle concentration (CMC) and were effective in removing both protein films, which can be explained following the orogenic mechanism. Results showed that the two-step UF process was effective to achieve high purity and fully functional surfactin. |
| first_indexed | 2025-11-14T23:22:25Z |
| format | Article |
| id | oai:generic.eprints.org:2639 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T23:22:25Z |
| publishDate | 2011 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:26392016-12-14T06:32:13Z http://journalarticle.ukm.my/2639/ Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process Mohd. Hafez Mohd Isa, Richard Frazier .A, Paula Jauregi, B. subtilis under certain types of media and fermentation conditions can produce surfactin, a biosurfactant which belongs to the lipopeptide class. Surfactin has exceptional surfactant activity, and exhibits some interesting biological characteristics such as antibacterial activity, antitumoral activity against ascites carcinoma cells, and a hypocholesterolemic activity that inhibits cAMP phosphodiesterase, as well as having anti-HIV properties. A cost effective recovery and purification of surfactin from fermentation broth using a two-step ultrafiltration (UF) process has been developed in order to reduce the cost of surfactin production. In this study, competitive adsorption of surfactin and proteins at the air-water interface was studied using surface pressure measurements. Small volumes of bovine serum albumin (BSA) and β-casein solutions were added to the air-water interface on a Langmuir trough and allowed to stabilise before the addition of surfactin to the subphase. Contrasting interfacial behaviour of proteins was observed with β-casein showing faster initial adsorption compared to BSA. On introduction of surfactin both proteins were displaced but a longer time were taken to displace β-casein. Overall the results showed surfactin were highly surface-active by forming a β-sheet structure at the air-water interface after reaching its critical micelle concentration (CMC) and were effective in removing both protein films, which can be explained following the orogenic mechanism. Results showed that the two-step UF process was effective to achieve high purity and fully functional surfactin. Universiti Kebangsaan Malaysia 2011-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/2639/1/15_Mohd_Hafez.pdf Mohd. Hafez Mohd Isa, and Richard Frazier .A, and Paula Jauregi, (2011) Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process. Sains Malaysiana, 40 (9). pp. 1043-1048. ISSN 0126-6039 http:www.ukm.my/jsm/ |
| spellingShingle | Mohd. Hafez Mohd Isa, Richard Frazier .A, Paula Jauregi, Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process |
| title | Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process |
| title_full | Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process |
| title_fullStr | Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process |
| title_full_unstemmed | Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process |
| title_short | Surface activity of surfactin recovered and purified from fermentation broth using a two-step Ultrafiltration (Uf) Process |
| title_sort | surface activity of surfactin recovered and purified from fermentation broth using a two-step ultrafiltration (uf) process |
| url | http://journalarticle.ukm.my/2639/ http://journalarticle.ukm.my/2639/ http://journalarticle.ukm.my/2639/1/15_Mohd_Hafez.pdf |