Evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast
Cryopreservation is a vital process for long-term preservation of cells without compromising their viability and functionality. Continuous cell culturing can lead to reduction in cell viability, higher risk of contamination, and increased reagent consumption. Optimizing short-term storage is essenti...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25987/ http://journalarticle.ukm.my/25987/1/SML%209.pdf |
| _version_ | 1848816499619266560 |
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| author | Tithteeya Rattanachot, Nur Rasyidah Hazimah Mohd Rosdi, Nusaibah Sallehuddin, Fauzi Mh Busra, Manira Maarof, |
| author_facet | Tithteeya Rattanachot, Nur Rasyidah Hazimah Mohd Rosdi, Nusaibah Sallehuddin, Fauzi Mh Busra, Manira Maarof, |
| author_sort | Tithteeya Rattanachot, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Cryopreservation is a vital process for long-term preservation of cells without compromising their viability and functionality. Continuous cell culturing can lead to reduction in cell viability, higher risk of contamination, and increased reagent consumption. Optimizing short-term storage is essential to minimize cell damage and enhance cell adaptability for applications requiring brief storage duration. This study evaluates the effects of short-term storage on human dermal fibroblasts cryopreserved in different cryopreservation media. Redundant skin samples were obtained from surgeries with patient consent, processed, and sub-cultured to passage three (P3). Confluent cells were trypsinised and cryopreserved in three cryopreservation media: Foetal bovine serum with 10% dimethyl sulfoxide (FBS+10%DMSO), CryoStor10 (CS10), and cryo freezing serum-free media (CF-SFM). Cells were stored at -80 °C for 7 days, 14 days, and 1 month. Fibroblasts maintained their spindle-shaped, elongated morphology across all groups post-storage. The total number of live cells slightly decreased after 1 month, but no significant differences were found between the groups. Cell viability in CS10 after 1 month was significantly lower compared to the other storage durations, while no significant differences were observed in the other two media groups. Immunocytochemistry showed positive collagen type I (Col-1) and Ki67 expression at all storage durations. These findings suggest that fibroblasts retain their characteristics after short-term storage at -80 °C in different cryopreservation media. However, further studies are needed to examine the impact of long-term storage on other cell types. |
| first_indexed | 2025-11-15T01:06:51Z |
| format | Article |
| id | oai:generic.eprints.org:25987 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:06:51Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:259872025-10-13T07:52:41Z http://journalarticle.ukm.my/25987/ Evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast Tithteeya Rattanachot, Nur Rasyidah Hazimah Mohd Rosdi, Nusaibah Sallehuddin, Fauzi Mh Busra, Manira Maarof, Cryopreservation is a vital process for long-term preservation of cells without compromising their viability and functionality. Continuous cell culturing can lead to reduction in cell viability, higher risk of contamination, and increased reagent consumption. Optimizing short-term storage is essential to minimize cell damage and enhance cell adaptability for applications requiring brief storage duration. This study evaluates the effects of short-term storage on human dermal fibroblasts cryopreserved in different cryopreservation media. Redundant skin samples were obtained from surgeries with patient consent, processed, and sub-cultured to passage three (P3). Confluent cells were trypsinised and cryopreserved in three cryopreservation media: Foetal bovine serum with 10% dimethyl sulfoxide (FBS+10%DMSO), CryoStor10 (CS10), and cryo freezing serum-free media (CF-SFM). Cells were stored at -80 °C for 7 days, 14 days, and 1 month. Fibroblasts maintained their spindle-shaped, elongated morphology across all groups post-storage. The total number of live cells slightly decreased after 1 month, but no significant differences were found between the groups. Cell viability in CS10 after 1 month was significantly lower compared to the other storage durations, while no significant differences were observed in the other two media groups. Immunocytochemistry showed positive collagen type I (Col-1) and Ki67 expression at all storage durations. These findings suggest that fibroblasts retain their characteristics after short-term storage at -80 °C in different cryopreservation media. However, further studies are needed to examine the impact of long-term storage on other cell types. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25987/1/SML%209.pdf Tithteeya Rattanachot, and Nur Rasyidah Hazimah Mohd Rosdi, and Nusaibah Sallehuddin, and Fauzi Mh Busra, and Manira Maarof, (2025) Evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast. Sains Malaysiana, 54 (8). pp. 1985-1994. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num8_2025/contentsVol54num8_2025.html |
| spellingShingle | Tithteeya Rattanachot, Nur Rasyidah Hazimah Mohd Rosdi, Nusaibah Sallehuddin, Fauzi Mh Busra, Manira Maarof, Evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast |
| title | Evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast |
| title_full | Evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast |
| title_fullStr | Evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast |
| title_full_unstemmed | Evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast |
| title_short | Evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast |
| title_sort | evaluating the efficacy of cryopreservation media for the preservation and short-term storage of human dermal fibroblast |
| url | http://journalarticle.ukm.my/25987/ http://journalarticle.ukm.my/25987/ http://journalarticle.ukm.my/25987/1/SML%209.pdf |