Inhibitory effect of Trichoderma spp. causing green mold disease on the edible mushroom Pleurotus pulmonarius and Pleurotus floridanus
The popular cultivated edible oyster mushrooms in Malaysia, Pleurotus pulmonarius (grey oyster mushroom) and Pleurotus floridanus (white oyster mushroom), are susceptible to green mold disease caused by Trichoderma spp. The decline of the quality and yield production of the mushrooms up to 100% has...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25981/ http://journalarticle.ukm.my/25981/1/SML%203.pdf |
| _version_ | 1848816498040111104 |
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| author | Shan, Wan Swan Fasehah Mohd Mulana, Jaya Seelan Sathiya Seelan, Tan, Yee Shin |
| author_facet | Shan, Wan Swan Fasehah Mohd Mulana, Jaya Seelan Sathiya Seelan, Tan, Yee Shin |
| author_sort | Shan, Wan Swan |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The popular cultivated edible oyster mushrooms in Malaysia, Pleurotus pulmonarius (grey oyster mushroom) and Pleurotus floridanus (white oyster mushroom), are susceptible to green mold disease caused by Trichoderma spp. The decline of the quality and yield production of the mushrooms up to 100% has been frequently observed due to infection by Trichoderma. The objective of this study was to investigate the competitive inhibition of Trichoderma spp. isolated from the farm with Pleurotus spp. Nine strains of Trichoderma were cultured in vitro with Pleurotus on potato dextrose agar and the radial growth as well as mycelial diameter growth were recorded. The direct plate assay results showed that T. koningiopsis and T. harzianum were the most pathogenic strains against P. pulmonarius and P. floridanus by overgrowing and replacing the mycelia, respectively, due to space competition and nutrient suppression. The bi-plate Petri dish approach for assessing contactless inhibition of Trichoderma spp. against Pleurotus spp. demonstrated that T. asperellum, T. ghanense, and T. koningiopsis were able to cross over the partition and inhibit P. pulmonarius mycelium. Meanwhile, inverted plate assay demonstrated that T. ghanense and T. reesei inhibited P. pulmonarius mycelia, respectively, indicating contactless inhibition of Pleurotus by Trichoderma. When tested with P. floridanus, only T. ghanense demonstrated inhibition in bi-plate Petri dish method and inverted plate assay. In summary, all nine Trichoderma spp. suppressed Pleurotus growth in varying degrees. Thus, besides competing with mushroom mycelia for nutrients and space, Trichoderma sp. could also release volatile organic compounds that act without direct contact. |
| first_indexed | 2025-11-15T01:06:49Z |
| format | Article |
| id | oai:generic.eprints.org:25981 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:06:49Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:259812025-10-13T07:03:34Z http://journalarticle.ukm.my/25981/ Inhibitory effect of Trichoderma spp. causing green mold disease on the edible mushroom Pleurotus pulmonarius and Pleurotus floridanus Shan, Wan Swan Fasehah Mohd Mulana, Jaya Seelan Sathiya Seelan, Tan, Yee Shin The popular cultivated edible oyster mushrooms in Malaysia, Pleurotus pulmonarius (grey oyster mushroom) and Pleurotus floridanus (white oyster mushroom), are susceptible to green mold disease caused by Trichoderma spp. The decline of the quality and yield production of the mushrooms up to 100% has been frequently observed due to infection by Trichoderma. The objective of this study was to investigate the competitive inhibition of Trichoderma spp. isolated from the farm with Pleurotus spp. Nine strains of Trichoderma were cultured in vitro with Pleurotus on potato dextrose agar and the radial growth as well as mycelial diameter growth were recorded. The direct plate assay results showed that T. koningiopsis and T. harzianum were the most pathogenic strains against P. pulmonarius and P. floridanus by overgrowing and replacing the mycelia, respectively, due to space competition and nutrient suppression. The bi-plate Petri dish approach for assessing contactless inhibition of Trichoderma spp. against Pleurotus spp. demonstrated that T. asperellum, T. ghanense, and T. koningiopsis were able to cross over the partition and inhibit P. pulmonarius mycelium. Meanwhile, inverted plate assay demonstrated that T. ghanense and T. reesei inhibited P. pulmonarius mycelia, respectively, indicating contactless inhibition of Pleurotus by Trichoderma. When tested with P. floridanus, only T. ghanense demonstrated inhibition in bi-plate Petri dish method and inverted plate assay. In summary, all nine Trichoderma spp. suppressed Pleurotus growth in varying degrees. Thus, besides competing with mushroom mycelia for nutrients and space, Trichoderma sp. could also release volatile organic compounds that act without direct contact. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25981/1/SML%203.pdf Shan, Wan Swan and Fasehah Mohd Mulana, and Jaya Seelan Sathiya Seelan, and Tan, Yee Shin (2025) Inhibitory effect of Trichoderma spp. causing green mold disease on the edible mushroom Pleurotus pulmonarius and Pleurotus floridanus. Sains Malaysiana, 54 (8). pp. 1901-1912. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num8_2025/contentsVol54num8_2025.html |
| spellingShingle | Shan, Wan Swan Fasehah Mohd Mulana, Jaya Seelan Sathiya Seelan, Tan, Yee Shin Inhibitory effect of Trichoderma spp. causing green mold disease on the edible mushroom Pleurotus pulmonarius and Pleurotus floridanus |
| title | Inhibitory effect of Trichoderma spp. causing green mold disease on the edible mushroom Pleurotus pulmonarius and Pleurotus floridanus |
| title_full | Inhibitory effect of Trichoderma spp. causing green mold disease on the edible mushroom Pleurotus pulmonarius and Pleurotus floridanus |
| title_fullStr | Inhibitory effect of Trichoderma spp. causing green mold disease on the edible mushroom Pleurotus pulmonarius and Pleurotus floridanus |
| title_full_unstemmed | Inhibitory effect of Trichoderma spp. causing green mold disease on the edible mushroom Pleurotus pulmonarius and Pleurotus floridanus |
| title_short | Inhibitory effect of Trichoderma spp. causing green mold disease on the edible mushroom Pleurotus pulmonarius and Pleurotus floridanus |
| title_sort | inhibitory effect of trichoderma spp. causing green mold disease on the edible mushroom pleurotus pulmonarius and pleurotus floridanus |
| url | http://journalarticle.ukm.my/25981/ http://journalarticle.ukm.my/25981/ http://journalarticle.ukm.my/25981/1/SML%203.pdf |