Thermal adaptation response of Glaciozyma antarctica PI12: statistical analysis of gene expression under temperature conditions
Glaciozyma antarctica PI12, a psychrophilic yeast isolated from Antarctic Sea ice, exhibits remarkable cold adaptation mechanisms. While its optimal growth occurs at 12 °C, its transcriptional response across broader temperature ranges remains poorly understood. We analysed the expression patterns o...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25913/ http://journalarticle.ukm.my/25913/1/SMT%2017.pdf |
| _version_ | 1848816481841709056 |
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| author | Nurul Sarah Izzati Zahid, Nora Muda, |
| author_facet | Nurul Sarah Izzati Zahid, Nora Muda, |
| author_sort | Nurul Sarah Izzati Zahid, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Glaciozyma antarctica PI12, a psychrophilic yeast isolated from Antarctic Sea ice, exhibits remarkable cold adaptation mechanisms. While its optimal growth occurs at 12 °C, its transcriptional response across broader temperature ranges remains poorly understood. We analysed the expression patterns of 7,853 genes under varying temperatures (-12 °C to 20 °C) and exposure times (6 and 24 h) to understand thermal stress responses. Our findings showed that G. antarctica exhibits maximum transcriptional changes (182.4% increase in differentially expressed genes) at 12 °C after 24 h exposure compared to -12 °C/6 h, suggesting an optimal temperature range for cellular adaptation. Gene expression decreased at higher temperatures (16 °C & 20 °C), indicating potential thermal stress responses. Temporal analysis showed that 24-h exposure elicited stronger transcriptional responses compared to 6-h exposure, highlighting the time-dependent nature of thermal adaptation. Statistical modelling using negative binomial regression supported these biological observations. Our findings provide new insights into G. antarctica’s transcriptional response to thermal stress and establish a framework for analysing complex gene expression patterns in psychrophilic organisms. |
| first_indexed | 2025-11-15T01:06:34Z |
| format | Article |
| id | oai:generic.eprints.org:25913 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:06:34Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:259132025-09-17T07:28:09Z http://journalarticle.ukm.my/25913/ Thermal adaptation response of Glaciozyma antarctica PI12: statistical analysis of gene expression under temperature conditions Nurul Sarah Izzati Zahid, Nora Muda, Glaciozyma antarctica PI12, a psychrophilic yeast isolated from Antarctic Sea ice, exhibits remarkable cold adaptation mechanisms. While its optimal growth occurs at 12 °C, its transcriptional response across broader temperature ranges remains poorly understood. We analysed the expression patterns of 7,853 genes under varying temperatures (-12 °C to 20 °C) and exposure times (6 and 24 h) to understand thermal stress responses. Our findings showed that G. antarctica exhibits maximum transcriptional changes (182.4% increase in differentially expressed genes) at 12 °C after 24 h exposure compared to -12 °C/6 h, suggesting an optimal temperature range for cellular adaptation. Gene expression decreased at higher temperatures (16 °C & 20 °C), indicating potential thermal stress responses. Temporal analysis showed that 24-h exposure elicited stronger transcriptional responses compared to 6-h exposure, highlighting the time-dependent nature of thermal adaptation. Statistical modelling using negative binomial regression supported these biological observations. Our findings provide new insights into G. antarctica’s transcriptional response to thermal stress and establish a framework for analysing complex gene expression patterns in psychrophilic organisms. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25913/1/SMT%2017.pdf Nurul Sarah Izzati Zahid, and Nora Muda, (2025) Thermal adaptation response of Glaciozyma antarctica PI12: statistical analysis of gene expression under temperature conditions. Sains Malaysiana, 54 (7). pp. 1847-1857. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num7_2025/contentsVol54num7_2025.html |
| spellingShingle | Nurul Sarah Izzati Zahid, Nora Muda, Thermal adaptation response of Glaciozyma antarctica PI12: statistical analysis of gene expression under temperature conditions |
| title | Thermal adaptation response of Glaciozyma antarctica PI12: statistical analysis of gene expression under temperature conditions |
| title_full | Thermal adaptation response of Glaciozyma antarctica PI12: statistical analysis of gene expression under temperature conditions |
| title_fullStr | Thermal adaptation response of Glaciozyma antarctica PI12: statistical analysis of gene expression under temperature conditions |
| title_full_unstemmed | Thermal adaptation response of Glaciozyma antarctica PI12: statistical analysis of gene expression under temperature conditions |
| title_short | Thermal adaptation response of Glaciozyma antarctica PI12: statistical analysis of gene expression under temperature conditions |
| title_sort | thermal adaptation response of glaciozyma antarctica pi12: statistical analysis of gene expression under temperature conditions |
| url | http://journalarticle.ukm.my/25913/ http://journalarticle.ukm.my/25913/ http://journalarticle.ukm.my/25913/1/SMT%2017.pdf |