Antimalarial activity screening from endophytic fungus of red ginger (Zingiber officinale): in vitro and in silico studies
Diversity exploration of secondary metabolite compounds from plant endophytic fungi is expected to yield novel compounds that can efficiently overcome Plasmodium resistance. This study aimed to assess the antimalarial activity of the endophytic fungus derived from red ginger Zingiber officinale agai...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25812/ http://journalarticle.ukm.my/25812/1/SME%208.pdf |
| _version_ | 1848816456790179840 |
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| author | Artasasta, Muh Ade Faradilla, Diandra Mytha Rasyid, Herlina Listyorini, Dwi Putra, Wira Eka Handayani, Dian Riga, Riga Kuo, Ping-Chung Li, Hao-Ze Chang, Pei-Hung Fitri, Loeki Enggar Harwoko, Harwoko Endrawati, Heni |
| author_facet | Artasasta, Muh Ade Faradilla, Diandra Mytha Rasyid, Herlina Listyorini, Dwi Putra, Wira Eka Handayani, Dian Riga, Riga Kuo, Ping-Chung Li, Hao-Ze Chang, Pei-Hung Fitri, Loeki Enggar Harwoko, Harwoko Endrawati, Heni |
| author_sort | Artasasta, Muh Ade |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Diversity exploration of secondary metabolite compounds from plant endophytic fungi is expected to yield novel compounds that can efficiently overcome Plasmodium resistance. This study aimed to assess the antimalarial activity of the endophytic fungus derived from red ginger Zingiber officinale against Plasmodium berghei. Subsequently, the endophytic fungus was isolated from the sample using the dilution method, followed by evaporation. Nine isolates of endophytic fungi were successfully isolated that were assigned as JMR1, JMR2, JMR3, JMR5, JMB1, JMB2, JMB3, JMD1, and JMD2. The antimalarial efficacy showed that the JMR5 isolate exhibited significant activity in suppressing the proliferation of P. berghei. This activity was quantified by a per cent inhibition of 83.01% and an IC50 value of 5.81 μg/mL. The detected antimalarial activity of the JMR5 extract can be related to the presence of several phytochemicals, including alkaloids, flavonoids, and terpenoids. In addition, molecular identification was conducted using ITS primers on the JMR5 isolate, showing a complete genetic similarity of 100% with Aspergillus flavus. GNPS analysis was conducted using LCMS-MS data on ethyl acetate extract. Surafactin c, surafactin c14 and erucamide were probably secondary metabolites in the JMR5 extract. Furthermore, drug-likeness and molecular docking analysis were conducted. The result showed that erucamide is a potential antimalarial due to the fulfil of Lipinski’s rule of five and also the binding affinity (- 4.2 kcal/mol) against Plasmepsin I. Based on the results obtained, the development of secondary metabolites from Aspergillus flavus JMR5 as potential antimalarial compounds is important to carry out. |
| first_indexed | 2025-11-15T01:06:10Z |
| format | Article |
| id | oai:generic.eprints.org:25812 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:06:10Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:258122025-09-04T03:28:47Z http://journalarticle.ukm.my/25812/ Antimalarial activity screening from endophytic fungus of red ginger (Zingiber officinale): in vitro and in silico studies Artasasta, Muh Ade Faradilla, Diandra Mytha Rasyid, Herlina Listyorini, Dwi Putra, Wira Eka Handayani, Dian Riga, Riga Kuo, Ping-Chung Li, Hao-Ze Chang, Pei-Hung Fitri, Loeki Enggar Harwoko, Harwoko Endrawati, Heni Diversity exploration of secondary metabolite compounds from plant endophytic fungi is expected to yield novel compounds that can efficiently overcome Plasmodium resistance. This study aimed to assess the antimalarial activity of the endophytic fungus derived from red ginger Zingiber officinale against Plasmodium berghei. Subsequently, the endophytic fungus was isolated from the sample using the dilution method, followed by evaporation. Nine isolates of endophytic fungi were successfully isolated that were assigned as JMR1, JMR2, JMR3, JMR5, JMB1, JMB2, JMB3, JMD1, and JMD2. The antimalarial efficacy showed that the JMR5 isolate exhibited significant activity in suppressing the proliferation of P. berghei. This activity was quantified by a per cent inhibition of 83.01% and an IC50 value of 5.81 μg/mL. The detected antimalarial activity of the JMR5 extract can be related to the presence of several phytochemicals, including alkaloids, flavonoids, and terpenoids. In addition, molecular identification was conducted using ITS primers on the JMR5 isolate, showing a complete genetic similarity of 100% with Aspergillus flavus. GNPS analysis was conducted using LCMS-MS data on ethyl acetate extract. Surafactin c, surafactin c14 and erucamide were probably secondary metabolites in the JMR5 extract. Furthermore, drug-likeness and molecular docking analysis were conducted. The result showed that erucamide is a potential antimalarial due to the fulfil of Lipinski’s rule of five and also the binding affinity (- 4.2 kcal/mol) against Plasmepsin I. Based on the results obtained, the development of secondary metabolites from Aspergillus flavus JMR5 as potential antimalarial compounds is important to carry out. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25812/1/SME%208.pdf Artasasta, Muh Ade and Faradilla, Diandra Mytha and Rasyid, Herlina and Listyorini, Dwi and Putra, Wira Eka and Handayani, Dian and Riga, Riga and Kuo, Ping-Chung and Li, Hao-Ze and Chang, Pei-Hung and Fitri, Loeki Enggar and Harwoko, Harwoko and Endrawati, Heni (2025) Antimalarial activity screening from endophytic fungus of red ginger (Zingiber officinale): in vitro and in silico studies. Sains Malaysiana, 54 (6). pp. 1523-1534. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num6_2025/contentsVol54num6_2025.html |
| spellingShingle | Artasasta, Muh Ade Faradilla, Diandra Mytha Rasyid, Herlina Listyorini, Dwi Putra, Wira Eka Handayani, Dian Riga, Riga Kuo, Ping-Chung Li, Hao-Ze Chang, Pei-Hung Fitri, Loeki Enggar Harwoko, Harwoko Endrawati, Heni Antimalarial activity screening from endophytic fungus of red ginger (Zingiber officinale): in vitro and in silico studies |
| title | Antimalarial activity screening from endophytic fungus of red ginger (Zingiber officinale): in vitro and in silico studies |
| title_full | Antimalarial activity screening from endophytic fungus of red ginger (Zingiber officinale): in vitro and in silico studies |
| title_fullStr | Antimalarial activity screening from endophytic fungus of red ginger (Zingiber officinale): in vitro and in silico studies |
| title_full_unstemmed | Antimalarial activity screening from endophytic fungus of red ginger (Zingiber officinale): in vitro and in silico studies |
| title_short | Antimalarial activity screening from endophytic fungus of red ginger (Zingiber officinale): in vitro and in silico studies |
| title_sort | antimalarial activity screening from endophytic fungus of red ginger (zingiber officinale): in vitro and in silico studies |
| url | http://journalarticle.ukm.my/25812/ http://journalarticle.ukm.my/25812/ http://journalarticle.ukm.my/25812/1/SME%208.pdf |