Potential of macang (Mangifera foetida) bark extract on antioxidant levels and pro-apoptotic proteins in rats (Rattus norvegicus) exposed to polystyrene nanoplastics

Nanoplastics in the environment primarily originate from contaminated aquatic animals, detergent waste, and cosmetic products, which can accumulate in the body through ingestion, inhalation, and skin exposure. This accumulation poses significant health risks, including reproductive health disruption...

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Main Authors: Safitri, Maya, Nurbani, Farah Annisa, Lulhaq, Hidayaturrohman Mulvi, Jamaluddin, Faiz, Wulandari, Khairani Resti, Pramudya, Manikya, Dewi, Firli Rahmah Primula, Lim, Vuanghao, Hayati, Alfiah
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2025
Online Access:http://journalarticle.ukm.my/25808/
http://journalarticle.ukm.my/25808/1/SME%204.pdf
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author Safitri, Maya
Nurbani, Farah Annisa
Lulhaq, Hidayaturrohman Mulvi
Jamaluddin, Faiz
Wulandari, Khairani Resti
Pramudya, Manikya
Dewi, Firli Rahmah Primula
Lim, Vuanghao
Hayati, Alfiah
author_facet Safitri, Maya
Nurbani, Farah Annisa
Lulhaq, Hidayaturrohman Mulvi
Jamaluddin, Faiz
Wulandari, Khairani Resti
Pramudya, Manikya
Dewi, Firli Rahmah Primula
Lim, Vuanghao
Hayati, Alfiah
author_sort Safitri, Maya
building UKM Institutional Repository
collection Online Access
description Nanoplastics in the environment primarily originate from contaminated aquatic animals, detergent waste, and cosmetic products, which can accumulate in the body through ingestion, inhalation, and skin exposure. This accumulation poses significant health risks, including reproductive health disruptions. This study aims to assess the potential of Mangifera foetida (macang) bark extract as an antioxidant to enhance immune and reproductive health in rats exposed to polystyrene nanoplastics (NP). Twenty-five male rats were divided into five groups: a control group, a negative control (10 μL/kg NP), and three treatment groups receiving 10 μL/kg NP with 50, 100, or 200 mg/kg of M. foetida extract for 35 days. The DPPH assay showed an IC50 value of 22.176 ppm, indicating strong antioxidant activity of the bark extract. Exposure to NP reduced the levels of endogenous antioxidant enzymes (SOD and CAT) and increased pro-apoptotic proteins (Bax and Caspase-3), affecting the histological structure and size of the testes. Treatment with M. foetida extract restored SOD and CAT levels while reducing Bax and Caspase-3 levels, improving histological structure by increasing the epithelium thickness and seminiferous tubule diameter. These findings suggest that M. foetida bark extract has potent antioxidant properties and can mitigate NP-induced toxicity, making it a promising protective agent.
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spelling oai:generic.eprints.org:258082025-09-11T03:39:59Z http://journalarticle.ukm.my/25808/ Potential of macang (Mangifera foetida) bark extract on antioxidant levels and pro-apoptotic proteins in rats (Rattus norvegicus) exposed to polystyrene nanoplastics Safitri, Maya Nurbani, Farah Annisa Lulhaq, Hidayaturrohman Mulvi Jamaluddin, Faiz Wulandari, Khairani Resti Pramudya, Manikya Dewi, Firli Rahmah Primula Lim, Vuanghao Hayati, Alfiah Nanoplastics in the environment primarily originate from contaminated aquatic animals, detergent waste, and cosmetic products, which can accumulate in the body through ingestion, inhalation, and skin exposure. This accumulation poses significant health risks, including reproductive health disruptions. This study aims to assess the potential of Mangifera foetida (macang) bark extract as an antioxidant to enhance immune and reproductive health in rats exposed to polystyrene nanoplastics (NP). Twenty-five male rats were divided into five groups: a control group, a negative control (10 μL/kg NP), and three treatment groups receiving 10 μL/kg NP with 50, 100, or 200 mg/kg of M. foetida extract for 35 days. The DPPH assay showed an IC50 value of 22.176 ppm, indicating strong antioxidant activity of the bark extract. Exposure to NP reduced the levels of endogenous antioxidant enzymes (SOD and CAT) and increased pro-apoptotic proteins (Bax and Caspase-3), affecting the histological structure and size of the testes. Treatment with M. foetida extract restored SOD and CAT levels while reducing Bax and Caspase-3 levels, improving histological structure by increasing the epithelium thickness and seminiferous tubule diameter. These findings suggest that M. foetida bark extract has potent antioxidant properties and can mitigate NP-induced toxicity, making it a promising protective agent. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25808/1/SME%204.pdf Safitri, Maya and Nurbani, Farah Annisa and Lulhaq, Hidayaturrohman Mulvi and Jamaluddin, Faiz and Wulandari, Khairani Resti and Pramudya, Manikya and Dewi, Firli Rahmah Primula and Lim, Vuanghao and Hayati, Alfiah (2025) Potential of macang (Mangifera foetida) bark extract on antioxidant levels and pro-apoptotic proteins in rats (Rattus norvegicus) exposed to polystyrene nanoplastics. Sains Malaysiana, 54 (6). pp. 1477-1487. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num6_2025/contentsVol54num6_2025.html
spellingShingle Safitri, Maya
Nurbani, Farah Annisa
Lulhaq, Hidayaturrohman Mulvi
Jamaluddin, Faiz
Wulandari, Khairani Resti
Pramudya, Manikya
Dewi, Firli Rahmah Primula
Lim, Vuanghao
Hayati, Alfiah
Potential of macang (Mangifera foetida) bark extract on antioxidant levels and pro-apoptotic proteins in rats (Rattus norvegicus) exposed to polystyrene nanoplastics
title Potential of macang (Mangifera foetida) bark extract on antioxidant levels and pro-apoptotic proteins in rats (Rattus norvegicus) exposed to polystyrene nanoplastics
title_full Potential of macang (Mangifera foetida) bark extract on antioxidant levels and pro-apoptotic proteins in rats (Rattus norvegicus) exposed to polystyrene nanoplastics
title_fullStr Potential of macang (Mangifera foetida) bark extract on antioxidant levels and pro-apoptotic proteins in rats (Rattus norvegicus) exposed to polystyrene nanoplastics
title_full_unstemmed Potential of macang (Mangifera foetida) bark extract on antioxidant levels and pro-apoptotic proteins in rats (Rattus norvegicus) exposed to polystyrene nanoplastics
title_short Potential of macang (Mangifera foetida) bark extract on antioxidant levels and pro-apoptotic proteins in rats (Rattus norvegicus) exposed to polystyrene nanoplastics
title_sort potential of macang (mangifera foetida) bark extract on antioxidant levels and pro-apoptotic proteins in rats (rattus norvegicus) exposed to polystyrene nanoplastics
url http://journalarticle.ukm.my/25808/
http://journalarticle.ukm.my/25808/
http://journalarticle.ukm.my/25808/1/SME%204.pdf