DNA barcoding shows taxonomic uncertainty in North Sumatran Mahseer, Neolissochilus: towards comprehensive revision in Indonesia
The mahseer species Neolissochilus holds significant ecological and socioeconomic value, but its wild population is declining. A major challenge in conservation efforts is taxonomy uncertainty. This study used DNA barcoding of three mitochondrial DNA (mtDNA) segments (COI, Cyt b, and 16S rRNA) to ve...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25806/ http://journalarticle.ukm.my/25806/1/SME%202.pdf |
| _version_ | 1848816455154401280 |
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| author | Larashati, Sekar Wahyudewantoro, Gema Widoretno, Mey Ristanti Febrianti, Dwi Marnis, Huria Barus, Ternala Alexander Sinuhaji, Yolanda Simaretha Christiany Kusumah, Ruby Vidia Samir, Octavianto Widiyanto, Tri |
| author_facet | Larashati, Sekar Wahyudewantoro, Gema Widoretno, Mey Ristanti Febrianti, Dwi Marnis, Huria Barus, Ternala Alexander Sinuhaji, Yolanda Simaretha Christiany Kusumah, Ruby Vidia Samir, Octavianto Widiyanto, Tri |
| author_sort | Larashati, Sekar |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The mahseer species Neolissochilus holds significant ecological and socioeconomic value, but its wild population is declining. A major challenge in conservation efforts is taxonomy uncertainty. This study used DNA barcoding of three mitochondrial DNA (mtDNA) segments (COI, Cyt b, and 16S rRNA) to verify the morphological identification of nine specimens collected from Bonan Dolok River and eight Neolissochilus sumatranus from the Tulas River, as well as three Neolissochilus soro samples from the Tulas River, and eight from Boho River, respectively, in North Sumatera. Morphological identification was based on body height, eye diameter/length, length of pectoral fin to the dorsal fin, anal fin to the caudal fin, and dorsal fin branch rays. Neighbour Joining analysis was used to construct the phylogenetic trees, showing that samples of N. sumatranus and N. soro clustered with supported bootstrap values of 63-71%, and no genetic distance between them. ASAP and ABGD species delimitation supported this clustering. This suggests both are the same species and closely related to N. soroides and N. hendersoni (genetic distance: 0.001-0.012 and 0.000-0.002, respectively). This challenges existing taxonomy and emphasizes the need to revisit Neolissochilus classification in Indonesia. Further study involving traditional taxonomy and DNA barcoding on Neolissochilus species in Indonesia is needed to clarify species distinction, validate taxonomy, and update the conservation status. This approach will enhance species identification, guide conservation efforts, and improve management of these vital freshwater fish species. |
| first_indexed | 2025-11-15T01:06:08Z |
| format | Article |
| id | oai:generic.eprints.org:25806 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:06:08Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:258062025-09-04T01:59:31Z http://journalarticle.ukm.my/25806/ DNA barcoding shows taxonomic uncertainty in North Sumatran Mahseer, Neolissochilus: towards comprehensive revision in Indonesia Larashati, Sekar Wahyudewantoro, Gema Widoretno, Mey Ristanti Febrianti, Dwi Marnis, Huria Barus, Ternala Alexander Sinuhaji, Yolanda Simaretha Christiany Kusumah, Ruby Vidia Samir, Octavianto Widiyanto, Tri The mahseer species Neolissochilus holds significant ecological and socioeconomic value, but its wild population is declining. A major challenge in conservation efforts is taxonomy uncertainty. This study used DNA barcoding of three mitochondrial DNA (mtDNA) segments (COI, Cyt b, and 16S rRNA) to verify the morphological identification of nine specimens collected from Bonan Dolok River and eight Neolissochilus sumatranus from the Tulas River, as well as three Neolissochilus soro samples from the Tulas River, and eight from Boho River, respectively, in North Sumatera. Morphological identification was based on body height, eye diameter/length, length of pectoral fin to the dorsal fin, anal fin to the caudal fin, and dorsal fin branch rays. Neighbour Joining analysis was used to construct the phylogenetic trees, showing that samples of N. sumatranus and N. soro clustered with supported bootstrap values of 63-71%, and no genetic distance between them. ASAP and ABGD species delimitation supported this clustering. This suggests both are the same species and closely related to N. soroides and N. hendersoni (genetic distance: 0.001-0.012 and 0.000-0.002, respectively). This challenges existing taxonomy and emphasizes the need to revisit Neolissochilus classification in Indonesia. Further study involving traditional taxonomy and DNA barcoding on Neolissochilus species in Indonesia is needed to clarify species distinction, validate taxonomy, and update the conservation status. This approach will enhance species identification, guide conservation efforts, and improve management of these vital freshwater fish species. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25806/1/SME%202.pdf Larashati, Sekar and Wahyudewantoro, Gema and Widoretno, Mey Ristanti and Febrianti, Dwi and Marnis, Huria and Barus, Ternala Alexander and Sinuhaji, Yolanda Simaretha Christiany and Kusumah, Ruby Vidia and Samir, Octavianto and Widiyanto, Tri (2025) DNA barcoding shows taxonomic uncertainty in North Sumatran Mahseer, Neolissochilus: towards comprehensive revision in Indonesia. Sains Malaysiana, 54 (6). pp. 1451-1464. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num6_2025/contentsVol54num6_2025.html |
| spellingShingle | Larashati, Sekar Wahyudewantoro, Gema Widoretno, Mey Ristanti Febrianti, Dwi Marnis, Huria Barus, Ternala Alexander Sinuhaji, Yolanda Simaretha Christiany Kusumah, Ruby Vidia Samir, Octavianto Widiyanto, Tri DNA barcoding shows taxonomic uncertainty in North Sumatran Mahseer, Neolissochilus: towards comprehensive revision in Indonesia |
| title | DNA barcoding shows taxonomic uncertainty in North Sumatran Mahseer, Neolissochilus: towards comprehensive revision in Indonesia |
| title_full | DNA barcoding shows taxonomic uncertainty in North Sumatran Mahseer, Neolissochilus: towards comprehensive revision in Indonesia |
| title_fullStr | DNA barcoding shows taxonomic uncertainty in North Sumatran Mahseer, Neolissochilus: towards comprehensive revision in Indonesia |
| title_full_unstemmed | DNA barcoding shows taxonomic uncertainty in North Sumatran Mahseer, Neolissochilus: towards comprehensive revision in Indonesia |
| title_short | DNA barcoding shows taxonomic uncertainty in North Sumatran Mahseer, Neolissochilus: towards comprehensive revision in Indonesia |
| title_sort | dna barcoding shows taxonomic uncertainty in north sumatran mahseer, neolissochilus: towards comprehensive revision in indonesia |
| url | http://journalarticle.ukm.my/25806/ http://journalarticle.ukm.my/25806/ http://journalarticle.ukm.my/25806/1/SME%202.pdf |