Gastroprotective effect and anti-apoptotic role of Lawsonia inermis extract against ethanol-induced gastric ulcers in Sprague Dawley
Lawsonia inermis (LI) or henna has been traditionally used since antiquity for cosmetics, medicinal and healing purposes including wound healing. Even so, the proof of the gastroprotective consequences of LI is inadequate while the pharmacological mechanisms continue to be scarce. The purpose of thi...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25636/ http://journalarticle.ukm.my/25636/1/SE%2013.pdf |
| _version_ | 1848816411962507264 |
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| author | Muhammad Remy Othman, Sayidah Asma Basir, Khadher Ahmad, Nur’ain Salehen, Giribabu Nelli, Rozana Othman, Zulkifli Mohd Yusoff, Syarifah Nur Syed Abdul Rahman, Hazrina Hazni, Khalijah Awang, |
| author_facet | Muhammad Remy Othman, Sayidah Asma Basir, Khadher Ahmad, Nur’ain Salehen, Giribabu Nelli, Rozana Othman, Zulkifli Mohd Yusoff, Syarifah Nur Syed Abdul Rahman, Hazrina Hazni, Khalijah Awang, |
| author_sort | Muhammad Remy Othman, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Lawsonia inermis (LI) or henna has been traditionally used since antiquity for cosmetics, medicinal and healing purposes including wound healing. Even so, the proof of the gastroprotective consequences of LI is inadequate while the pharmacological mechanisms continue to be scarce. The purpose of this research was to examine the acute toxicity and gastroprotective activities of Lawsonia inermis L. leaves ethanol:water (80:20) extract (LILEW) in contrast to ethanol-induced gastric ulcers within rats. The acute oral toxicity experiment was performed using Sprague Dawley female rats (n=6) for low dose (2000 mg/kg), and high dose (5000 mg/kg) as well as vehicle (distilled water). The gastroprotective result of LILEW was performed in an ethanol-induced ulcer inside Sprague Dawley rats (n=6). The animals were distributed among five groups namely ulcer control, normal control, drug control-omeprazole, high dose, and low dose of LILEW (500 mg/kg and 250 mg/kg, respectively). Acute toxicity experiments with LILEW on the rats did not demonstrate any signs of mortality and toxicity up to 5 g/kg suggesting that LILEW is safe to consume. The gastroprotective effect of LILEW at doses 250 mg/kg and 500 mg/kg, as well as omeprazole (20 mg/kg), showed significant rates of inhibition as much as 70.50±1.38% (drug control), 66.67±1.42% (low dose of LILEW) and 68.45±1.53% (high dose of LILEW) of rats attenuated gastric lesions that were generated by ethanol. In conclusion, these findings confirmed that Lawsonia inermis L. leaves ethanol: water extract is safe to consume and able to protect against gastric ulcers. |
| first_indexed | 2025-11-15T01:05:27Z |
| format | Article |
| id | oai:generic.eprints.org:25636 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:05:27Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:256362025-07-16T08:01:41Z http://journalarticle.ukm.my/25636/ Gastroprotective effect and anti-apoptotic role of Lawsonia inermis extract against ethanol-induced gastric ulcers in Sprague Dawley Muhammad Remy Othman, Sayidah Asma Basir, Khadher Ahmad, Nur’ain Salehen, Giribabu Nelli, Rozana Othman, Zulkifli Mohd Yusoff, Syarifah Nur Syed Abdul Rahman, Hazrina Hazni, Khalijah Awang, Lawsonia inermis (LI) or henna has been traditionally used since antiquity for cosmetics, medicinal and healing purposes including wound healing. Even so, the proof of the gastroprotective consequences of LI is inadequate while the pharmacological mechanisms continue to be scarce. The purpose of this research was to examine the acute toxicity and gastroprotective activities of Lawsonia inermis L. leaves ethanol:water (80:20) extract (LILEW) in contrast to ethanol-induced gastric ulcers within rats. The acute oral toxicity experiment was performed using Sprague Dawley female rats (n=6) for low dose (2000 mg/kg), and high dose (5000 mg/kg) as well as vehicle (distilled water). The gastroprotective result of LILEW was performed in an ethanol-induced ulcer inside Sprague Dawley rats (n=6). The animals were distributed among five groups namely ulcer control, normal control, drug control-omeprazole, high dose, and low dose of LILEW (500 mg/kg and 250 mg/kg, respectively). Acute toxicity experiments with LILEW on the rats did not demonstrate any signs of mortality and toxicity up to 5 g/kg suggesting that LILEW is safe to consume. The gastroprotective effect of LILEW at doses 250 mg/kg and 500 mg/kg, as well as omeprazole (20 mg/kg), showed significant rates of inhibition as much as 70.50±1.38% (drug control), 66.67±1.42% (low dose of LILEW) and 68.45±1.53% (high dose of LILEW) of rats attenuated gastric lesions that were generated by ethanol. In conclusion, these findings confirmed that Lawsonia inermis L. leaves ethanol: water extract is safe to consume and able to protect against gastric ulcers. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25636/1/SE%2013.pdf Muhammad Remy Othman, and Sayidah Asma Basir, and Khadher Ahmad, and Nur’ain Salehen, and Giribabu Nelli, and Rozana Othman, and Zulkifli Mohd Yusoff, and Syarifah Nur Syed Abdul Rahman, and Hazrina Hazni, and Khalijah Awang, (2025) Gastroprotective effect and anti-apoptotic role of Lawsonia inermis extract against ethanol-induced gastric ulcers in Sprague Dawley. Sains Malaysiana, 54 (4). pp. 1127-1145. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num4_2025/contentsVol54num4_2025.html |
| spellingShingle | Muhammad Remy Othman, Sayidah Asma Basir, Khadher Ahmad, Nur’ain Salehen, Giribabu Nelli, Rozana Othman, Zulkifli Mohd Yusoff, Syarifah Nur Syed Abdul Rahman, Hazrina Hazni, Khalijah Awang, Gastroprotective effect and anti-apoptotic role of Lawsonia inermis extract against ethanol-induced gastric ulcers in Sprague Dawley |
| title | Gastroprotective effect and anti-apoptotic role of Lawsonia inermis extract against ethanol-induced gastric ulcers in Sprague Dawley |
| title_full | Gastroprotective effect and anti-apoptotic role of Lawsonia inermis extract against ethanol-induced gastric ulcers in Sprague Dawley |
| title_fullStr | Gastroprotective effect and anti-apoptotic role of Lawsonia inermis extract against ethanol-induced gastric ulcers in Sprague Dawley |
| title_full_unstemmed | Gastroprotective effect and anti-apoptotic role of Lawsonia inermis extract against ethanol-induced gastric ulcers in Sprague Dawley |
| title_short | Gastroprotective effect and anti-apoptotic role of Lawsonia inermis extract against ethanol-induced gastric ulcers in Sprague Dawley |
| title_sort | gastroprotective effect and anti-apoptotic role of lawsonia inermis extract against ethanol-induced gastric ulcers in sprague dawley |
| url | http://journalarticle.ukm.my/25636/ http://journalarticle.ukm.my/25636/ http://journalarticle.ukm.my/25636/1/SE%2013.pdf |