Profiling the non-targeted proteins in Sunda porcupine (Hystrix javanica) quills to identify the wound healing potential substance
Sunda porcupine (Hystrix javanica) has quill as the main appendage of its skin and used as a means of self-defence against predators. In some regions of Indonesia, roasted porcupine quills are employed as a traditional remedy for dental pain and wound healing. This treatment is likely to use protein...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25628/ http://journalarticle.ukm.my/25628/1/SE%205.pdf |
| _version_ | 1848816409772032000 |
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| author | Gifardi, Muhammad Dirga Sutardi, Lina Noviyanti Farida, Wartika Rosa Prawira, Andhika Yudha Maulana, Tulus Mazaya, Maulida Agungpriyono, Srihadi |
| author_facet | Gifardi, Muhammad Dirga Sutardi, Lina Noviyanti Farida, Wartika Rosa Prawira, Andhika Yudha Maulana, Tulus Mazaya, Maulida Agungpriyono, Srihadi |
| author_sort | Gifardi, Muhammad Dirga |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Sunda porcupine (Hystrix javanica) has quill as the main appendage of its skin and used as a means of self-defence against predators. In some regions of Indonesia, roasted porcupine quills are employed as a traditional remedy for dental pain and wound healing. This treatment is likely to use protein and other compounds in the quills that interact directly with tissues to produce pharmacological effects. The aim of this study was profiling the protein composition in the porcupine quills in order to screening the potential substances which helps improve wound healing. Natural detached porcupine quills were collected and extracted for total protein. The protein composition was identified using SDS-PAGE and LC-MS/MS instrument. The data were analyzed using bioinformatics approach. This study demonstrates that porcupine quills have a soluble protein concentration of 2.11 ± 1.14 mg/mL composed mainly of proteins with molecular weights of 245 kDa and 60 kDa. LC-MS/MS utilisation showed the presence of 19 suspected proteins, including keratin 1, 2, 5, 6a, 8, 10, 14, 17, and 84. Bioinformatic analysis showed that three proteins with more than 1 unique peptide were potentially affecting wound healing, which were Keratin Type II, cytoskeletal 1 (Keratin 1); Keratin Type II, cytoskeletal 6a (Keratin 6a); and Annexin. The results of study provide scientific evidence regarding the potential substance of porcupine quills to help improve wound healing. |
| first_indexed | 2025-11-15T01:05:25Z |
| format | Article |
| id | oai:generic.eprints.org:25628 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:05:25Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:256282025-07-16T01:55:58Z http://journalarticle.ukm.my/25628/ Profiling the non-targeted proteins in Sunda porcupine (Hystrix javanica) quills to identify the wound healing potential substance Gifardi, Muhammad Dirga Sutardi, Lina Noviyanti Farida, Wartika Rosa Prawira, Andhika Yudha Maulana, Tulus Mazaya, Maulida Agungpriyono, Srihadi Sunda porcupine (Hystrix javanica) has quill as the main appendage of its skin and used as a means of self-defence against predators. In some regions of Indonesia, roasted porcupine quills are employed as a traditional remedy for dental pain and wound healing. This treatment is likely to use protein and other compounds in the quills that interact directly with tissues to produce pharmacological effects. The aim of this study was profiling the protein composition in the porcupine quills in order to screening the potential substances which helps improve wound healing. Natural detached porcupine quills were collected and extracted for total protein. The protein composition was identified using SDS-PAGE and LC-MS/MS instrument. The data were analyzed using bioinformatics approach. This study demonstrates that porcupine quills have a soluble protein concentration of 2.11 ± 1.14 mg/mL composed mainly of proteins with molecular weights of 245 kDa and 60 kDa. LC-MS/MS utilisation showed the presence of 19 suspected proteins, including keratin 1, 2, 5, 6a, 8, 10, 14, 17, and 84. Bioinformatic analysis showed that three proteins with more than 1 unique peptide were potentially affecting wound healing, which were Keratin Type II, cytoskeletal 1 (Keratin 1); Keratin Type II, cytoskeletal 6a (Keratin 6a); and Annexin. The results of study provide scientific evidence regarding the potential substance of porcupine quills to help improve wound healing. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25628/1/SE%205.pdf Gifardi, Muhammad Dirga and Sutardi, Lina Noviyanti and Farida, Wartika Rosa and Prawira, Andhika Yudha and Maulana, Tulus and Mazaya, Maulida and Agungpriyono, Srihadi (2025) Profiling the non-targeted proteins in Sunda porcupine (Hystrix javanica) quills to identify the wound healing potential substance. Sains Malaysiana, 54 (4). pp. 1025-1036. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num4_2025/contentsVol54num4_2025.html |
| spellingShingle | Gifardi, Muhammad Dirga Sutardi, Lina Noviyanti Farida, Wartika Rosa Prawira, Andhika Yudha Maulana, Tulus Mazaya, Maulida Agungpriyono, Srihadi Profiling the non-targeted proteins in Sunda porcupine (Hystrix javanica) quills to identify the wound healing potential substance |
| title | Profiling the non-targeted proteins in Sunda porcupine (Hystrix javanica) quills to identify the wound healing potential substance |
| title_full | Profiling the non-targeted proteins in Sunda porcupine (Hystrix javanica) quills to identify the wound healing potential substance |
| title_fullStr | Profiling the non-targeted proteins in Sunda porcupine (Hystrix javanica) quills to identify the wound healing potential substance |
| title_full_unstemmed | Profiling the non-targeted proteins in Sunda porcupine (Hystrix javanica) quills to identify the wound healing potential substance |
| title_short | Profiling the non-targeted proteins in Sunda porcupine (Hystrix javanica) quills to identify the wound healing potential substance |
| title_sort | profiling the non-targeted proteins in sunda porcupine (hystrix javanica) quills to identify the wound healing potential substance |
| url | http://journalarticle.ukm.my/25628/ http://journalarticle.ukm.my/25628/ http://journalarticle.ukm.my/25628/1/SE%205.pdf |