Curcumin improves the expression of phospholiphase C and transient receptor potential vanilloid type 1 on neuronal myometrium in dysmenorrhea model
The pathogenesis of primary dysmenorrhea is closely related to increased expression of phospholipase C (PLC) and transient receptor potential vanilloid 1 (TRPV1) in myometrial nerve cells. Curcumin has traditionally been used to reduce the pain associated with dysmenorrhea. To date, the effect of cu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25310/ http://journalarticle.ukm.my/25310/1/ST%2020.pdf |
| _version_ | 1848816322832498688 |
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| author | Mukhoirotin, Purba, Abdul Khairul Rizki Purwanto, Bambang Ernawati, Zainiyah, Hamimatus Gutjahr, Lina Patricia |
| author_facet | Mukhoirotin, Purba, Abdul Khairul Rizki Purwanto, Bambang Ernawati, Zainiyah, Hamimatus Gutjahr, Lina Patricia |
| author_sort | Mukhoirotin, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The pathogenesis of primary dysmenorrhea is closely related to increased expression of phospholipase C (PLC) and transient receptor potential vanilloid 1 (TRPV1) in myometrial nerve cells. Curcumin has traditionally been used to reduce the pain associated with dysmenorrhea. To date, the effect of curcumin on the expression of PLC and TRPV1 in myometrial pain sensitization has not been studied. This study aimed to determine the impact of curcumin on the expression of PLC and TRPV1 in the myometrial nerves of a primary dysmenorrhea model. The study involved a randomized post-test control group design using non-pregnant female Balb/c mice as a model of primary dysmenorrhea induced by estradiol benzoate and oxytocin. Thirty-five mice that met the inclusion criteria were divided into five groups: sham group (SG), receiving placebo; ibuprofen group (IG) receiving ibuprofen 100 mg/kg; Cur100 receiving curcumin 100 mg/kg; Cur200 receiving curcumin 200 mg/kg; and Cur400 receiving curcumin 400 mg/kg. Each drug was administered orally twice daily for 7 days. Finally, oxytocin was administered to induce a writhing response in the mice. PLC and TRPV1 expression were examined using double immunofluorescence staining. Data were analyzed using a one-way analysis of variance and Tukey’s test. Curcumin significantly lowered PLC and TRPV1 expressions in the primary dysmenorrhea model compared to those in the SG (p<0.05). The best effect was observed in the Cur400 group with the PLC expression at 27.31+7.42 (p<0.001), and TRPV1 expression at 33.21±9.99 (p<0.001). In a primary dysmenorrhea model, Curcumin effectively improves PLC and TRPV1 expression in myometrial nerves. |
| first_indexed | 2025-11-15T01:04:02Z |
| format | Article |
| id | oai:generic.eprints.org:25310 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:04:02Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
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| spelling | oai:generic.eprints.org:253102025-05-22T01:34:53Z http://journalarticle.ukm.my/25310/ Curcumin improves the expression of phospholiphase C and transient receptor potential vanilloid type 1 on neuronal myometrium in dysmenorrhea model Mukhoirotin, Purba, Abdul Khairul Rizki Purwanto, Bambang Ernawati, Zainiyah, Hamimatus Gutjahr, Lina Patricia The pathogenesis of primary dysmenorrhea is closely related to increased expression of phospholipase C (PLC) and transient receptor potential vanilloid 1 (TRPV1) in myometrial nerve cells. Curcumin has traditionally been used to reduce the pain associated with dysmenorrhea. To date, the effect of curcumin on the expression of PLC and TRPV1 in myometrial pain sensitization has not been studied. This study aimed to determine the impact of curcumin on the expression of PLC and TRPV1 in the myometrial nerves of a primary dysmenorrhea model. The study involved a randomized post-test control group design using non-pregnant female Balb/c mice as a model of primary dysmenorrhea induced by estradiol benzoate and oxytocin. Thirty-five mice that met the inclusion criteria were divided into five groups: sham group (SG), receiving placebo; ibuprofen group (IG) receiving ibuprofen 100 mg/kg; Cur100 receiving curcumin 100 mg/kg; Cur200 receiving curcumin 200 mg/kg; and Cur400 receiving curcumin 400 mg/kg. Each drug was administered orally twice daily for 7 days. Finally, oxytocin was administered to induce a writhing response in the mice. PLC and TRPV1 expression were examined using double immunofluorescence staining. Data were analyzed using a one-way analysis of variance and Tukey’s test. Curcumin significantly lowered PLC and TRPV1 expressions in the primary dysmenorrhea model compared to those in the SG (p<0.05). The best effect was observed in the Cur400 group with the PLC expression at 27.31+7.42 (p<0.001), and TRPV1 expression at 33.21±9.99 (p<0.001). In a primary dysmenorrhea model, Curcumin effectively improves PLC and TRPV1 expression in myometrial nerves. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25310/1/ST%2020.pdf Mukhoirotin, and Purba, Abdul Khairul Rizki and Purwanto, Bambang and Ernawati, and Zainiyah, Hamimatus and Gutjahr, Lina Patricia (2025) Curcumin improves the expression of phospholiphase C and transient receptor potential vanilloid type 1 on neuronal myometrium in dysmenorrhea model. Sains Malaysiana, 54 (2). pp. 557-567. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num2_2025/contentsVol54num2_2025.html |
| spellingShingle | Mukhoirotin, Purba, Abdul Khairul Rizki Purwanto, Bambang Ernawati, Zainiyah, Hamimatus Gutjahr, Lina Patricia Curcumin improves the expression of phospholiphase C and transient receptor potential vanilloid type 1 on neuronal myometrium in dysmenorrhea model |
| title | Curcumin improves the expression of phospholiphase C and transient receptor potential vanilloid type 1 on neuronal myometrium in dysmenorrhea model |
| title_full | Curcumin improves the expression of phospholiphase C and transient receptor potential vanilloid type 1 on neuronal myometrium in dysmenorrhea model |
| title_fullStr | Curcumin improves the expression of phospholiphase C and transient receptor potential vanilloid type 1 on neuronal myometrium in dysmenorrhea model |
| title_full_unstemmed | Curcumin improves the expression of phospholiphase C and transient receptor potential vanilloid type 1 on neuronal myometrium in dysmenorrhea model |
| title_short | Curcumin improves the expression of phospholiphase C and transient receptor potential vanilloid type 1 on neuronal myometrium in dysmenorrhea model |
| title_sort | curcumin improves the expression of phospholiphase c and transient receptor potential vanilloid type 1 on neuronal myometrium in dysmenorrhea model |
| url | http://journalarticle.ukm.my/25310/ http://journalarticle.ukm.my/25310/ http://journalarticle.ukm.my/25310/1/ST%2020.pdf |