Genetic diversity of garut mutant (Maranta arundinacea L.) (M3) with random amplified polymorphic DNA (RAPD) analysis
Arrowroot is a type of tuber that is rich in carbohydrates. This plant can grow well in various parts of Indonesia. However, arrowroot has a low level of genetic diversity, making it difficult to obtain superior varieties with high productivity. Mutation treatment with gamma-ray radiation is expecte...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25301/ http://journalarticle.ukm.my/25301/1/ST%2011.pdf |
| _version_ | 1848816320045383680 |
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| author | Paradisa, Yashanti Berlinda Deswina, Puspita Priadi, Dodi Anggraheni, Yuliana Galih Dyan Nurbaeti, Santi Chaidir, Liberty Wicaksana, Noladhi |
| author_facet | Paradisa, Yashanti Berlinda Deswina, Puspita Priadi, Dodi Anggraheni, Yuliana Galih Dyan Nurbaeti, Santi Chaidir, Liberty Wicaksana, Noladhi |
| author_sort | Paradisa, Yashanti Berlinda |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Arrowroot is a type of tuber that is rich in carbohydrates. This plant can grow well in various parts of Indonesia. However, arrowroot has a low level of genetic diversity, making it difficult to obtain superior varieties with high productivity. Mutation treatment with gamma-ray radiation is expected to increase the diversity of arrowroot to produce superior-quality plants. This study aimed to determine the genetic diversity of arrowroot mutants resulting from the 4th generation of gamma-ray radiation (M3). The research analysed molecular markers using Random Amplified Polymorphic DNA (RAPD). A total of 30 samples of arrowroot M3 DNA were amplified using 11 selected primers. The results of the analysis show that there are six specific primers selected from the selection, which have the potential to produce high polymorphism bands that can be used for diversity analysis. The similarity index of arrowroot mutant is 0.69 to 0.99, which means that it still has a high similarity between arrowroot mutant. Based on genetic similarity of 84%, mutant arrowroot (M3) can be divided into 7 clusters. |
| first_indexed | 2025-11-15T01:04:00Z |
| format | Article |
| id | oai:generic.eprints.org:25301 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:04:00Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:253012025-05-29T06:38:44Z http://journalarticle.ukm.my/25301/ Genetic diversity of garut mutant (Maranta arundinacea L.) (M3) with random amplified polymorphic DNA (RAPD) analysis Paradisa, Yashanti Berlinda Deswina, Puspita Priadi, Dodi Anggraheni, Yuliana Galih Dyan Nurbaeti, Santi Chaidir, Liberty Wicaksana, Noladhi Arrowroot is a type of tuber that is rich in carbohydrates. This plant can grow well in various parts of Indonesia. However, arrowroot has a low level of genetic diversity, making it difficult to obtain superior varieties with high productivity. Mutation treatment with gamma-ray radiation is expected to increase the diversity of arrowroot to produce superior-quality plants. This study aimed to determine the genetic diversity of arrowroot mutants resulting from the 4th generation of gamma-ray radiation (M3). The research analysed molecular markers using Random Amplified Polymorphic DNA (RAPD). A total of 30 samples of arrowroot M3 DNA were amplified using 11 selected primers. The results of the analysis show that there are six specific primers selected from the selection, which have the potential to produce high polymorphism bands that can be used for diversity analysis. The similarity index of arrowroot mutant is 0.69 to 0.99, which means that it still has a high similarity between arrowroot mutant. Based on genetic similarity of 84%, mutant arrowroot (M3) can be divided into 7 clusters. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25301/1/ST%2011.pdf Paradisa, Yashanti Berlinda and Deswina, Puspita and Priadi, Dodi and Anggraheni, Yuliana Galih Dyan and Nurbaeti, Santi and Chaidir, Liberty and Wicaksana, Noladhi (2025) Genetic diversity of garut mutant (Maranta arundinacea L.) (M3) with random amplified polymorphic DNA (RAPD) analysis. Sains Malaysiana, 54 (2). pp. 449-460. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num2_2025/contentsVol54num2_2025.html |
| spellingShingle | Paradisa, Yashanti Berlinda Deswina, Puspita Priadi, Dodi Anggraheni, Yuliana Galih Dyan Nurbaeti, Santi Chaidir, Liberty Wicaksana, Noladhi Genetic diversity of garut mutant (Maranta arundinacea L.) (M3) with random amplified polymorphic DNA (RAPD) analysis |
| title | Genetic diversity of garut mutant (Maranta arundinacea L.) (M3) with random amplified polymorphic DNA (RAPD) analysis |
| title_full | Genetic diversity of garut mutant (Maranta arundinacea L.) (M3) with random amplified polymorphic DNA (RAPD) analysis |
| title_fullStr | Genetic diversity of garut mutant (Maranta arundinacea L.) (M3) with random amplified polymorphic DNA (RAPD) analysis |
| title_full_unstemmed | Genetic diversity of garut mutant (Maranta arundinacea L.) (M3) with random amplified polymorphic DNA (RAPD) analysis |
| title_short | Genetic diversity of garut mutant (Maranta arundinacea L.) (M3) with random amplified polymorphic DNA (RAPD) analysis |
| title_sort | genetic diversity of garut mutant (maranta arundinacea l.) (m3) with random amplified polymorphic dna (rapd) analysis |
| url | http://journalarticle.ukm.my/25301/ http://journalarticle.ukm.my/25301/ http://journalarticle.ukm.my/25301/1/ST%2011.pdf |