Polyphenol-rich extract of roselle improves oxidative stress status, renal function and structure of diabetic rat
Diabetic nephropathy is a progressive chronic renal disease, that leads to renal failure mediated by oxidative stress. Roselle or Hibiscus sabdariffa is well-known for its health benefits and is rich in polyphenols with high antioxidant activities. Limited studies evaluate the effects of polyphenol-...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2025
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| Online Access: | http://journalarticle.ukm.my/25071/ http://journalarticle.ukm.my/25071/1/SSb%2019.pdf |
| _version_ | 1848816261031526400 |
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| author | Fatima Mohammed Yusr, Fatin Farhana Jubaidi, Sumayyah Ismail, Siti Balkis Budin, |
| author_facet | Fatima Mohammed Yusr, Fatin Farhana Jubaidi, Sumayyah Ismail, Siti Balkis Budin, |
| author_sort | Fatima Mohammed Yusr, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Diabetic nephropathy is a progressive chronic renal disease, that leads to renal failure mediated by oxidative stress. Roselle or Hibiscus sabdariffa is well-known for its health benefits and is rich in polyphenols with high antioxidant activities. Limited studies evaluate the effects of polyphenol-rich extract of roselle (HPE) on the development of diabetic nephropathy. Hence, this study aimed to determine the effect of HPE in the renal of diabetic rats focusing on oxidative stress, renal function, and structural changes. Type 1 diabetes mellitus was induced in adult male Sprague-Dawley rats which were then divided into three groups: untreated diabetes (DM), HPE-supplemented diabetic rats (DM+HPE), and metformin-treated diabetic rats (DM+MET). Non-diabetic rats (NDM) served as normal controls. All rats were left untreated for four weeks followed by another four weeks of treatment, respectively. At the end of the study, all rats were sacrificed whereby blood was collected for creatinine level analysis, and kidneys were collected for oxidative stress and antioxidant markers as well as histological analyses. Data showed that HPE reduced blood glucose and creatinine levels significantly and improved catalase (CAT) activity. HPE supplementation also reduced the lipid and protein oxidation process, marked by significant reductions in malondialdehyde (MDA) and advanced oxidation protein products (AOPP). Moreover, HPE supplementation improved renal structure, especially in reducing glomerulus degeneration and renal tubule damage. In conclusion, this study suggests that HPE is able to reduce renal damage by mitigating oxidative stress and improving antioxidant status that potentially prevents the progression of diabetic nephropathy in diabetic rat model. |
| first_indexed | 2025-11-15T01:03:03Z |
| format | Article |
| id | oai:generic.eprints.org:25071 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:03:03Z |
| publishDate | 2025 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:250712025-04-08T08:20:33Z http://journalarticle.ukm.my/25071/ Polyphenol-rich extract of roselle improves oxidative stress status, renal function and structure of diabetic rat Fatima Mohammed Yusr, Fatin Farhana Jubaidi, Sumayyah Ismail, Siti Balkis Budin, Diabetic nephropathy is a progressive chronic renal disease, that leads to renal failure mediated by oxidative stress. Roselle or Hibiscus sabdariffa is well-known for its health benefits and is rich in polyphenols with high antioxidant activities. Limited studies evaluate the effects of polyphenol-rich extract of roselle (HPE) on the development of diabetic nephropathy. Hence, this study aimed to determine the effect of HPE in the renal of diabetic rats focusing on oxidative stress, renal function, and structural changes. Type 1 diabetes mellitus was induced in adult male Sprague-Dawley rats which were then divided into three groups: untreated diabetes (DM), HPE-supplemented diabetic rats (DM+HPE), and metformin-treated diabetic rats (DM+MET). Non-diabetic rats (NDM) served as normal controls. All rats were left untreated for four weeks followed by another four weeks of treatment, respectively. At the end of the study, all rats were sacrificed whereby blood was collected for creatinine level analysis, and kidneys were collected for oxidative stress and antioxidant markers as well as histological analyses. Data showed that HPE reduced blood glucose and creatinine levels significantly and improved catalase (CAT) activity. HPE supplementation also reduced the lipid and protein oxidation process, marked by significant reductions in malondialdehyde (MDA) and advanced oxidation protein products (AOPP). Moreover, HPE supplementation improved renal structure, especially in reducing glomerulus degeneration and renal tubule damage. In conclusion, this study suggests that HPE is able to reduce renal damage by mitigating oxidative stress and improving antioxidant status that potentially prevents the progression of diabetic nephropathy in diabetic rat model. Penerbit Universiti Kebangsaan Malaysia 2025 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/25071/1/SSb%2019.pdf Fatima Mohammed Yusr, and Fatin Farhana Jubaidi, and Sumayyah Ismail, and Siti Balkis Budin, (2025) Polyphenol-rich extract of roselle improves oxidative stress status, renal function and structure of diabetic rat. Sains Malaysiana, 54 (1). pp. 243-253. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol54num1_2025/contentsVol54num1_2025.html |
| spellingShingle | Fatima Mohammed Yusr, Fatin Farhana Jubaidi, Sumayyah Ismail, Siti Balkis Budin, Polyphenol-rich extract of roselle improves oxidative stress status, renal function and structure of diabetic rat |
| title | Polyphenol-rich extract of roselle improves oxidative stress status, renal function and structure of diabetic rat |
| title_full | Polyphenol-rich extract of roselle improves oxidative stress status, renal function and structure of diabetic rat |
| title_fullStr | Polyphenol-rich extract of roselle improves oxidative stress status, renal function and structure of diabetic rat |
| title_full_unstemmed | Polyphenol-rich extract of roselle improves oxidative stress status, renal function and structure of diabetic rat |
| title_short | Polyphenol-rich extract of roselle improves oxidative stress status, renal function and structure of diabetic rat |
| title_sort | polyphenol-rich extract of roselle improves oxidative stress status, renal function and structure of diabetic rat |
| url | http://journalarticle.ukm.my/25071/ http://journalarticle.ukm.my/25071/ http://journalarticle.ukm.my/25071/1/SSb%2019.pdf |