A preliminary screening of single nucleotide polymorphism (SNP) of A1 and A2 beta-casein alleles in dairy cattle
Milk containing A1 variants in the beta-casein gene is considered to be a risk factor for coronary heart disease (CHD), type I diabetes (DM-I), autism, and schizophrenia due to the release of beta-casomorphin 7 (BCM-7). The objectives of this study were to screen for polymorphism of beta-casein in d...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24997/ http://journalarticle.ukm.my/24997/1/MAE%2011.pdf |
| _version_ | 1848816240286498816 |
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| author | Nadiatur Akmar Zulkifli, Nurhafizatul Ain Baharim, Sharifah Salmah Syed Hussain, |
| author_facet | Nadiatur Akmar Zulkifli, Nurhafizatul Ain Baharim, Sharifah Salmah Syed Hussain, |
| author_sort | Nadiatur Akmar Zulkifli, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Milk containing A1 variants in the beta-casein gene is considered to be a risk factor for coronary heart disease (CHD), type I diabetes (DM-I), autism, and schizophrenia due to the release of beta-casomorphin 7 (BCM-7). The objectives of this study were to screen for polymorphism of beta-casein in dairy cattle and to examine the allelic and genotypic frequencies of A1 and A2 variants. A total of 290 cattle blood samples were collected from two states and the samples were extracted. This was followed by PCR amplification for exon 7 of the beta-casein gene (CSN2). The PCR products were then subjected to DNA sequencing. The sequencing results were aligned and the variants were screened. In Pahang, 125 samples are of the A2A2 genotype, followed by A1A2 (n=97) and A1A1 (n=28). While in Melaka, the highest genotype was A2A2 (n=20) as compared to A1A2 (n=18) and A1A1 (n=2). Meanwhile, the frequency of the A2 allele was higher than A1 in both states. The high genotypic frequencies of A2A2 in these cattle populations could be influenced by the cattle breed as well as the rate of crossbreeding on the farm. Thus, screening of dairy cattle for A1 and A2 beta-casein could be used in selecting the preferred animals for breeding. |
| first_indexed | 2025-11-15T01:02:44Z |
| format | Article |
| id | oai:generic.eprints.org:24997 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:02:44Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:249972025-03-21T01:30:02Z http://journalarticle.ukm.my/24997/ A preliminary screening of single nucleotide polymorphism (SNP) of A1 and A2 beta-casein alleles in dairy cattle Nadiatur Akmar Zulkifli, Nurhafizatul Ain Baharim, Sharifah Salmah Syed Hussain, Milk containing A1 variants in the beta-casein gene is considered to be a risk factor for coronary heart disease (CHD), type I diabetes (DM-I), autism, and schizophrenia due to the release of beta-casomorphin 7 (BCM-7). The objectives of this study were to screen for polymorphism of beta-casein in dairy cattle and to examine the allelic and genotypic frequencies of A1 and A2 variants. A total of 290 cattle blood samples were collected from two states and the samples were extracted. This was followed by PCR amplification for exon 7 of the beta-casein gene (CSN2). The PCR products were then subjected to DNA sequencing. The sequencing results were aligned and the variants were screened. In Pahang, 125 samples are of the A2A2 genotype, followed by A1A2 (n=97) and A1A1 (n=28). While in Melaka, the highest genotype was A2A2 (n=20) as compared to A1A2 (n=18) and A1A1 (n=2). Meanwhile, the frequency of the A2 allele was higher than A1 in both states. The high genotypic frequencies of A2A2 in these cattle populations could be influenced by the cattle breed as well as the rate of crossbreeding on the farm. Thus, screening of dairy cattle for A1 and A2 beta-casein could be used in selecting the preferred animals for breeding. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24997/1/MAE%2011.pdf Nadiatur Akmar Zulkifli, and Nurhafizatul Ain Baharim, and Sharifah Salmah Syed Hussain, (2024) A preliminary screening of single nucleotide polymorphism (SNP) of A1 and A2 beta-casein alleles in dairy cattle. Malaysian Applied Biology, 53 (6). pp. 115-121. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/65 |
| spellingShingle | Nadiatur Akmar Zulkifli, Nurhafizatul Ain Baharim, Sharifah Salmah Syed Hussain, A preliminary screening of single nucleotide polymorphism (SNP) of A1 and A2 beta-casein alleles in dairy cattle |
| title | A preliminary screening of single nucleotide polymorphism (SNP) of A1 and A2 beta-casein alleles in dairy cattle |
| title_full | A preliminary screening of single nucleotide polymorphism (SNP) of A1 and A2 beta-casein alleles in dairy cattle |
| title_fullStr | A preliminary screening of single nucleotide polymorphism (SNP) of A1 and A2 beta-casein alleles in dairy cattle |
| title_full_unstemmed | A preliminary screening of single nucleotide polymorphism (SNP) of A1 and A2 beta-casein alleles in dairy cattle |
| title_short | A preliminary screening of single nucleotide polymorphism (SNP) of A1 and A2 beta-casein alleles in dairy cattle |
| title_sort | preliminary screening of single nucleotide polymorphism (snp) of a1 and a2 beta-casein alleles in dairy cattle |
| url | http://journalarticle.ukm.my/24997/ http://journalarticle.ukm.my/24997/ http://journalarticle.ukm.my/24997/1/MAE%2011.pdf |