Isolation and antibacterial screening of marine heterotrophic bacteria from Makassar Strait, Indonesia
The biological potential of microorganisms in Indonesian waters has yet to be widely reported despite their high diversity. This research aimed to isolate bacteria from seawater and seabed sediments in Makassar Strait and then screen their potential antibacterial abilities. Subsequently, we tried to...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24938/ http://journalarticle.ukm.my/24938/1/SD%202.pdf |
| _version_ | 1848816224500187136 |
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| author | De Fretes, Charlie Ester Pesilette, Rosmi Nuslah Sohilait, Mario Rowan Yanubi, Dorthea Ririhatuela, Florensia Claudya Thalia Satwika, Dhira |
| author_facet | De Fretes, Charlie Ester Pesilette, Rosmi Nuslah Sohilait, Mario Rowan Yanubi, Dorthea Ririhatuela, Florensia Claudya Thalia Satwika, Dhira |
| author_sort | De Fretes, Charlie Ester |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The biological potential of microorganisms in Indonesian waters has yet to be widely reported despite their high diversity. This research aimed to isolate bacteria from seawater and seabed sediments in Makassar Strait and then screen their potential antibacterial abilities. Subsequently, we tried to determine the bioactive compound content of the bacterial extracts. We purified 15 bacterial isolates and based on a 16S rRNA analysis, we found that these bacteria represented three phyla, namely Actinobacteria, Firmicutes, and Proteobacteria. All the isolates were found to have antibacterial capabilities, while 2 isolates showed positive results for inhibiting the growth of the pathogens tested. Molecular analysis studies provided information that the two isolates which were Bacillus sp., namely B. stratosphericus TR4 and B. thuringiensis TR13, were detected to have non-ribosomal peptide synthetase (NRPS) genes, which are thought to contribute to their antibacterial potential. Compounds from the imidazole group, quinolone ester derivatives, steroid derivatives, and acetamide derivatives were found in the extracts of both strains. Further research needs to be conducted to better understand each compound’s antibacterial mechanisms and possible interesting bioactive properties. |
| first_indexed | 2025-11-15T01:02:29Z |
| format | Article |
| id | oai:generic.eprints.org:24938 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:02:29Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:249382025-03-17T02:21:43Z http://journalarticle.ukm.my/24938/ Isolation and antibacterial screening of marine heterotrophic bacteria from Makassar Strait, Indonesia De Fretes, Charlie Ester Pesilette, Rosmi Nuslah Sohilait, Mario Rowan Yanubi, Dorthea Ririhatuela, Florensia Claudya Thalia Satwika, Dhira The biological potential of microorganisms in Indonesian waters has yet to be widely reported despite their high diversity. This research aimed to isolate bacteria from seawater and seabed sediments in Makassar Strait and then screen their potential antibacterial abilities. Subsequently, we tried to determine the bioactive compound content of the bacterial extracts. We purified 15 bacterial isolates and based on a 16S rRNA analysis, we found that these bacteria represented three phyla, namely Actinobacteria, Firmicutes, and Proteobacteria. All the isolates were found to have antibacterial capabilities, while 2 isolates showed positive results for inhibiting the growth of the pathogens tested. Molecular analysis studies provided information that the two isolates which were Bacillus sp., namely B. stratosphericus TR4 and B. thuringiensis TR13, were detected to have non-ribosomal peptide synthetase (NRPS) genes, which are thought to contribute to their antibacterial potential. Compounds from the imidazole group, quinolone ester derivatives, steroid derivatives, and acetamide derivatives were found in the extracts of both strains. Further research needs to be conducted to better understand each compound’s antibacterial mechanisms and possible interesting bioactive properties. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24938/1/SD%202.pdf De Fretes, Charlie Ester and Pesilette, Rosmi Nuslah and Sohilait, Mario Rowan and Yanubi, Dorthea and Ririhatuela, Florensia Claudya Thalia and Satwika, Dhira (2024) Isolation and antibacterial screening of marine heterotrophic bacteria from Makassar Strait, Indonesia. Sains Malaysiana, 53 (12). pp. 3859-3870. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num12_2024/contentsVol53num12_2024.html |
| spellingShingle | De Fretes, Charlie Ester Pesilette, Rosmi Nuslah Sohilait, Mario Rowan Yanubi, Dorthea Ririhatuela, Florensia Claudya Thalia Satwika, Dhira Isolation and antibacterial screening of marine heterotrophic bacteria from Makassar Strait, Indonesia |
| title | Isolation and antibacterial screening of marine heterotrophic bacteria from Makassar Strait, Indonesia |
| title_full | Isolation and antibacterial screening of marine heterotrophic bacteria from Makassar Strait, Indonesia |
| title_fullStr | Isolation and antibacterial screening of marine heterotrophic bacteria from Makassar Strait, Indonesia |
| title_full_unstemmed | Isolation and antibacterial screening of marine heterotrophic bacteria from Makassar Strait, Indonesia |
| title_short | Isolation and antibacterial screening of marine heterotrophic bacteria from Makassar Strait, Indonesia |
| title_sort | isolation and antibacterial screening of marine heterotrophic bacteria from makassar strait, indonesia |
| url | http://journalarticle.ukm.my/24938/ http://journalarticle.ukm.my/24938/ http://journalarticle.ukm.my/24938/1/SD%202.pdf |