In silico elucidation of protein-protein interaction network in fish pathogen Flavobacterium columnare
Flavobacterium columnare is a virulent intracellular bacterial pathogen that causes an infection known as columnaris in many species of fish. Some economically important fish species are strongly affected by columnaris, leading to a high mortality rate and significant economic losses. Previous in si...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24741/ http://journalarticle.ukm.my/24741/1/MAT%2013.pdf |
| _version_ | 1848816174992719872 |
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| author | Pershia Nematiasgarabad, Nikman Adli Nor Hashim, Mohd Fakharul Zaman Raja Yahya, |
| author_facet | Pershia Nematiasgarabad, Nikman Adli Nor Hashim, Mohd Fakharul Zaman Raja Yahya, |
| author_sort | Pershia Nematiasgarabad, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Flavobacterium columnare is a virulent intracellular bacterial pathogen that causes an infection known as columnaris in many species of fish. Some economically important fish species are strongly affected by columnaris, leading to a high mortality rate and significant economic losses. Previous in silico studies have provided various biological insights into F. columnare, including its interaction with MHC class I alleles and the epitopic region within outer membrane proteins. However, the protein-protein interaction networks underlying the growth, defense, and pathogenesis of F. columnare remain largely unknown. This study was conducted to identify the protein-protein interaction (PPI) networks and hub proteins of F. columnare that can be used as drug or vaccine targets. A total of 500 protein sequences were retrieved from UniprotKB in FASTA format and analyzed using VaxiJen, PSORTb, STRING, Cytoscape, and BLASTp programs. The results demonstrated that 60% of F. columnare proteins were predicted as antigenic proteins, most of which were associated with catalytic activity and metabolic processes, identified as cytoplasmic proteins. Ten hub proteins with the highest number of functional interactions were identified, which were also antigenic and non-host homologous. In conclusion, F. columnare hub proteins represent potential therapeutic targets in drug and vaccine development against columnaris infection. |
| first_indexed | 2025-11-15T01:01:41Z |
| format | Article |
| id | oai:generic.eprints.org:24741 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T01:01:41Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:247412025-01-17T08:40:01Z http://journalarticle.ukm.my/24741/ In silico elucidation of protein-protein interaction network in fish pathogen Flavobacterium columnare Pershia Nematiasgarabad, Nikman Adli Nor Hashim, Mohd Fakharul Zaman Raja Yahya, Flavobacterium columnare is a virulent intracellular bacterial pathogen that causes an infection known as columnaris in many species of fish. Some economically important fish species are strongly affected by columnaris, leading to a high mortality rate and significant economic losses. Previous in silico studies have provided various biological insights into F. columnare, including its interaction with MHC class I alleles and the epitopic region within outer membrane proteins. However, the protein-protein interaction networks underlying the growth, defense, and pathogenesis of F. columnare remain largely unknown. This study was conducted to identify the protein-protein interaction (PPI) networks and hub proteins of F. columnare that can be used as drug or vaccine targets. A total of 500 protein sequences were retrieved from UniprotKB in FASTA format and analyzed using VaxiJen, PSORTb, STRING, Cytoscape, and BLASTp programs. The results demonstrated that 60% of F. columnare proteins were predicted as antigenic proteins, most of which were associated with catalytic activity and metabolic processes, identified as cytoplasmic proteins. Ten hub proteins with the highest number of functional interactions were identified, which were also antigenic and non-host homologous. In conclusion, F. columnare hub proteins represent potential therapeutic targets in drug and vaccine development against columnaris infection. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24741/1/MAT%2013.pdf Pershia Nematiasgarabad, and Nikman Adli Nor Hashim, and Mohd Fakharul Zaman Raja Yahya, (2024) In silico elucidation of protein-protein interaction network in fish pathogen Flavobacterium columnare. Malaysian Applied Biology, 53 (3). pp. 137-146. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/60 |
| spellingShingle | Pershia Nematiasgarabad, Nikman Adli Nor Hashim, Mohd Fakharul Zaman Raja Yahya, In silico elucidation of protein-protein interaction network in fish pathogen Flavobacterium columnare |
| title | In silico elucidation of protein-protein interaction network in fish pathogen Flavobacterium columnare |
| title_full | In silico elucidation of protein-protein interaction network in fish pathogen Flavobacterium columnare |
| title_fullStr | In silico elucidation of protein-protein interaction network in fish pathogen Flavobacterium columnare |
| title_full_unstemmed | In silico elucidation of protein-protein interaction network in fish pathogen Flavobacterium columnare |
| title_short | In silico elucidation of protein-protein interaction network in fish pathogen Flavobacterium columnare |
| title_sort | in silico elucidation of protein-protein interaction network in fish pathogen flavobacterium columnare |
| url | http://journalarticle.ukm.my/24741/ http://journalarticle.ukm.my/24741/ http://journalarticle.ukm.my/24741/1/MAT%2013.pdf |