Antibacterial potential of fungal endophytes from selected seaweeds from Johor Coast

Endophytic fungi from marine seaweed have been known to be the source of new secondary biological metabolites. The ample coast in Malaysia in particular the Johor Coast has diverse marine seaweed, home to potential marine endophytic fungi. In this light, this study aims to characterise endophytic fu...

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Main Authors: Muhammad Haziq Anwar Azlan, Nur Farah Ain Zainee, Nazlina Ibrahim
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/24730/
http://journalarticle.ukm.my/24730/1/MAT%202.pdf
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author Muhammad Haziq Anwar Azlan,
Nur Farah Ain Zainee,
Nazlina Ibrahim,
author_facet Muhammad Haziq Anwar Azlan,
Nur Farah Ain Zainee,
Nazlina Ibrahim,
author_sort Muhammad Haziq Anwar Azlan,
building UKM Institutional Repository
collection Online Access
description Endophytic fungi from marine seaweed have been known to be the source of new secondary biological metabolites. The ample coast in Malaysia in particular the Johor Coast has diverse marine seaweed, home to potential marine endophytic fungi. In this light, this study aims to characterise endophytic fungi in selected seaweeds from the Johor Coast and determine the antibacterial potential. Fungal endophytes were aseptically isolated from brown seaweed, Sargassum sp. and green algae, Ulva lactuca. Macroscopic and microscopic observations were performed for characterisation as fungal genera. Sequence analysis of Internal Transcribed Spacer (ITS) suggested the five fungal isolates as Trichoderma asperellum, Aspergillus aculeatus, Aspergillus fumigatus, Penicillium sp. FKI-3389 and Hypoxylon monticulosum. Antibacterial activity was determined by minimum inhibition concentration assay against five human pathogenic bacteria. Only T. asperellum, A. fumigatus and H. monticulosum showed antibacterial potential with the latter indicating broad-spectrum antibacterial activity. As a conclusion, five endophytic fungal species were successfully determined from the brown and green seaweeds. Three of the fungal endophytes showed potential in antibacterial activity with H. monticulosum displayed broad spectrum activity.
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spelling oai:generic.eprints.org:247302025-01-17T03:39:55Z http://journalarticle.ukm.my/24730/ Antibacterial potential of fungal endophytes from selected seaweeds from Johor Coast Muhammad Haziq Anwar Azlan, Nur Farah Ain Zainee, Nazlina Ibrahim, Endophytic fungi from marine seaweed have been known to be the source of new secondary biological metabolites. The ample coast in Malaysia in particular the Johor Coast has diverse marine seaweed, home to potential marine endophytic fungi. In this light, this study aims to characterise endophytic fungi in selected seaweeds from the Johor Coast and determine the antibacterial potential. Fungal endophytes were aseptically isolated from brown seaweed, Sargassum sp. and green algae, Ulva lactuca. Macroscopic and microscopic observations were performed for characterisation as fungal genera. Sequence analysis of Internal Transcribed Spacer (ITS) suggested the five fungal isolates as Trichoderma asperellum, Aspergillus aculeatus, Aspergillus fumigatus, Penicillium sp. FKI-3389 and Hypoxylon monticulosum. Antibacterial activity was determined by minimum inhibition concentration assay against five human pathogenic bacteria. Only T. asperellum, A. fumigatus and H. monticulosum showed antibacterial potential with the latter indicating broad-spectrum antibacterial activity. As a conclusion, five endophytic fungal species were successfully determined from the brown and green seaweeds. Three of the fungal endophytes showed potential in antibacterial activity with H. monticulosum displayed broad spectrum activity. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24730/1/MAT%202.pdf Muhammad Haziq Anwar Azlan, and Nur Farah Ain Zainee, and Nazlina Ibrahim, (2024) Antibacterial potential of fungal endophytes from selected seaweeds from Johor Coast. Malaysian Applied Biology, 53 (3). pp. 15-21. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/60
spellingShingle Muhammad Haziq Anwar Azlan,
Nur Farah Ain Zainee,
Nazlina Ibrahim,
Antibacterial potential of fungal endophytes from selected seaweeds from Johor Coast
title Antibacterial potential of fungal endophytes from selected seaweeds from Johor Coast
title_full Antibacterial potential of fungal endophytes from selected seaweeds from Johor Coast
title_fullStr Antibacterial potential of fungal endophytes from selected seaweeds from Johor Coast
title_full_unstemmed Antibacterial potential of fungal endophytes from selected seaweeds from Johor Coast
title_short Antibacterial potential of fungal endophytes from selected seaweeds from Johor Coast
title_sort antibacterial potential of fungal endophytes from selected seaweeds from johor coast
url http://journalarticle.ukm.my/24730/
http://journalarticle.ukm.my/24730/
http://journalarticle.ukm.my/24730/1/MAT%202.pdf