Exploring the therapeutic potential: Malaysian channa striatus water extract enriched with arachidonic acid for wound healing in human foetal lung cells (IMR-90)
Channa striatus, also known as haruan, is traditionally used in Malaysia for its wound-healing properties. This study evaluated the wound healing potential of Channa striatus water extract (CSWE) and identified key compounds promoting fibroblast cell growth. Channa striatus were deboned to max...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2024
|
| Online Access: | http://journalarticle.ukm.my/24481/ http://journalarticle.ukm.my/24481/1/Sains%20Kesihatan_22_2_7.pdf |
| Summary: | Channa striatus, also known as haruan, is traditionally used in Malaysia for its wound-healing
properties. This study evaluated the wound healing potential of Channa striatus water extract
(CSWE) and identified key compounds promoting fibroblast cell growth. Channa striatus were
deboned to maximize fillet retention. CSWE was obtained through aqueous extraction, and its
physicochemical properties were analyzed, including pH, rheological characteristics, moisture
content, amino acid composition, and arachidonic acid presence. The effective concentration
(EC50) of the sample was determined using a 2-D cell culture system with human fibroblast
cells (IMR-90) over three days. Results showed CSWE had a near-neutral pH (6.34 ± 0.01)
and high moisture content (97.3 ± 0.01%). The extract displayed Newtonian fluid behavior
with a viscosity of 1.50 ± 0.31 mPa.s. CSWE contained essential amino acid glycine and
arachidonic acid, important for wound healing, but in low concentrations. These low
concentrations did not significantly promote IMR-90 cell growth (p>0.05) compared to the
control. Consequently, EC50 values for CSWE were invalid due to over-dilution from a high
fish weight-to-solvent ratio. Despite this, IMR-90 cell growth rates remained consistent across
different CSWE concentrations, with no observed mortality during the three-day incubation.
Overall, IMR-90 cells exhibited insignificant growth, even with arachidonic acid and glycine
at low concentrations during treatment. |
|---|