Antioxidant activities and laxative effect of bioactive compounds from Cynara cardunculus var. sylvestris
The present study aims to investigate the antioxidant and anti-constipation activities of the derived phenolic extracts in ribs wild cardoon (Cynara sylvestris). Analysed extracts (acetone, methanol and aqueous) exhibited high level of phenolic compounds and excellent antioxidant activities with sig...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2024
|
| Online Access: | http://journalarticle.ukm.my/24243/ http://journalarticle.ukm.my/24243/1/ST%2011.pdf |
| _version_ | 1848816048255533056 |
|---|---|
| author | Tajini, Fatma Jelassi, Awatef Hamdani, Amal Salem, Ali Abdelhedi, Ola Ouerghui, Abid Sebai, Hichem |
| author_facet | Tajini, Fatma Jelassi, Awatef Hamdani, Amal Salem, Ali Abdelhedi, Ola Ouerghui, Abid Sebai, Hichem |
| author_sort | Tajini, Fatma |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The present study aims to investigate the antioxidant and anti-constipation activities of the derived phenolic extracts in ribs wild cardoon (Cynara sylvestris). Analysed extracts (acetone, methanol and aqueous) exhibited high level of phenolic compounds and excellent antioxidant activities with significant IC50 values (p≤0.05), as assessed by the DPPH radical-scavenging activity (0.84±0.08 - 2.44±0.007 μg/mL), ABTS cationic radical test (0.96 ±0.01- 136.67±6.75 μg/mL) and β-carotene bleaching assay (2.09±0.02-51.12±1.32 μg/mL). Also, higher levels of insoluble dietary fibers were found (56.18 ± 0.91% DW of neutral detergent fiber). The in vivo investigation was performed on Wistar rats to explore the ability of C. sylvestris aqueous extract (CSAE) in the enhancement of the gastrointestinal transit and the treatment of induced constipation by Loperamide (commercialized as Idium). A significant increase of 15, 24.5 and 32.4% in gastrointestinal motility was recorded when doses of CSAE increased (75, 150 and 300 mg/ kg b.w, respectively). Food intake, water consumption, number and weight of stools were also increased in a dosedependent manner, compared to the positive control (untreated). Moreover, CSAE provided significantly (p≤0.05) and dose-dependently protection against oxidative stress by preserving normal antioxidant enzymes activities (SOD and GPx) in intestinal and colonic mucosa, and resorted hepatic enzymes (AST and ALT) and renal (urea and creatinine) levels to normal values. These results can be explained by the abundance of phenolic compounds and insoluble fibers in this plant. Therefore, its use can be encouraged in alimentary and pharmaceutical applications as antioxidant and laxative food supplements. |
| first_indexed | 2025-11-15T00:59:40Z |
| format | Article |
| id | oai:generic.eprints.org:24243 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:59:40Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:242432024-09-24T08:08:36Z http://journalarticle.ukm.my/24243/ Antioxidant activities and laxative effect of bioactive compounds from Cynara cardunculus var. sylvestris Tajini, Fatma Jelassi, Awatef Hamdani, Amal Salem, Ali Abdelhedi, Ola Ouerghui, Abid Sebai, Hichem The present study aims to investigate the antioxidant and anti-constipation activities of the derived phenolic extracts in ribs wild cardoon (Cynara sylvestris). Analysed extracts (acetone, methanol and aqueous) exhibited high level of phenolic compounds and excellent antioxidant activities with significant IC50 values (p≤0.05), as assessed by the DPPH radical-scavenging activity (0.84±0.08 - 2.44±0.007 μg/mL), ABTS cationic radical test (0.96 ±0.01- 136.67±6.75 μg/mL) and β-carotene bleaching assay (2.09±0.02-51.12±1.32 μg/mL). Also, higher levels of insoluble dietary fibers were found (56.18 ± 0.91% DW of neutral detergent fiber). The in vivo investigation was performed on Wistar rats to explore the ability of C. sylvestris aqueous extract (CSAE) in the enhancement of the gastrointestinal transit and the treatment of induced constipation by Loperamide (commercialized as Idium). A significant increase of 15, 24.5 and 32.4% in gastrointestinal motility was recorded when doses of CSAE increased (75, 150 and 300 mg/ kg b.w, respectively). Food intake, water consumption, number and weight of stools were also increased in a dosedependent manner, compared to the positive control (untreated). Moreover, CSAE provided significantly (p≤0.05) and dose-dependently protection against oxidative stress by preserving normal antioxidant enzymes activities (SOD and GPx) in intestinal and colonic mucosa, and resorted hepatic enzymes (AST and ALT) and renal (urea and creatinine) levels to normal values. These results can be explained by the abundance of phenolic compounds and insoluble fibers in this plant. Therefore, its use can be encouraged in alimentary and pharmaceutical applications as antioxidant and laxative food supplements. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24243/1/ST%2011.pdf Tajini, Fatma and Jelassi, Awatef and Hamdani, Amal and Salem, Ali and Abdelhedi, Ola and Ouerghui, Abid and Sebai, Hichem (2024) Antioxidant activities and laxative effect of bioactive compounds from Cynara cardunculus var. sylvestris. Sains Malaysiana, 53 (7). pp. 1617-1630. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num7_2024/contentsVol53num7_2024.html |
| spellingShingle | Tajini, Fatma Jelassi, Awatef Hamdani, Amal Salem, Ali Abdelhedi, Ola Ouerghui, Abid Sebai, Hichem Antioxidant activities and laxative effect of bioactive compounds from Cynara cardunculus var. sylvestris |
| title | Antioxidant activities and laxative effect of bioactive compounds from Cynara cardunculus var. sylvestris |
| title_full | Antioxidant activities and laxative effect of bioactive compounds from Cynara cardunculus var. sylvestris |
| title_fullStr | Antioxidant activities and laxative effect of bioactive compounds from Cynara cardunculus var. sylvestris |
| title_full_unstemmed | Antioxidant activities and laxative effect of bioactive compounds from Cynara cardunculus var. sylvestris |
| title_short | Antioxidant activities and laxative effect of bioactive compounds from Cynara cardunculus var. sylvestris |
| title_sort | antioxidant activities and laxative effect of bioactive compounds from cynara cardunculus var. sylvestris |
| url | http://journalarticle.ukm.my/24243/ http://journalarticle.ukm.my/24243/ http://journalarticle.ukm.my/24243/1/ST%2011.pdf |