Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers
Banana (Musa spp.) is an economically important crop widely consumed all over the world. Understanding the genetic diversity and relatedness between various banana cultivars is important to continuously improve its fruit characteristics, nutritional value, and disease resistance. This study aimed to...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24242/ http://journalarticle.ukm.my/24242/1/ST%2010.pdf |
| _version_ | 1848816048011214848 |
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| author | Sharmilah Vetaryan, Hooi, Wei Yeng Lee, Yang Ping |
| author_facet | Sharmilah Vetaryan, Hooi, Wei Yeng Lee, Yang Ping |
| author_sort | Sharmilah Vetaryan, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Banana (Musa spp.) is an economically important crop widely consumed all over the world. Understanding the genetic diversity and relatedness between various banana cultivars is important to continuously improve its fruit characteristics, nutritional value, and disease resistance. This study aimed to develop a DNA fingerprinting panel using polymorphic simple sequence repeat (SSR) markers for the genetic characterisation of 11 banana cultivars in Malaysia. A total of 10 polymorphic SSR markers were developed into a multiplex PCR reaction and capillary electrophoresis to uniquely profile our collection of banana cultivars. The developed fingerprinting panel successfully amplified a total of 95 alleles, with 6 to 13 alleles per SSR marker. The average SSR marker polymorphic information content (PIC) value is 0.812, indicating the informativeness of the panel. Analysis of molecular variance (AMOVA) shows that 97% of the molecular variance from our banana collection is due to inter-variety genetic diversity, while the remaining 3% is due to intra-variety genetic diversity. A population structure analysis groups our collection of banana varieties according to the presence of at least one M. balbisiana (B) genome in their genetic makeup. The Cavendish variety, however, showed a distinct structure compared to the other cultivars. This SSR fingerprinting panel provides valuable insights into the genetic diversity and relatedness between banana cultivars in Malaysia. It has the potential to assist future banana breeding initiatives and serve as an effective quality control measure for verifying varieties in a tissue culture facility involved in banana planting materials production. |
| first_indexed | 2025-11-15T00:59:40Z |
| format | Article |
| id | oai:generic.eprints.org:24242 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:59:40Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:242422024-09-24T07:59:23Z http://journalarticle.ukm.my/24242/ Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers Sharmilah Vetaryan, Hooi, Wei Yeng Lee, Yang Ping Banana (Musa spp.) is an economically important crop widely consumed all over the world. Understanding the genetic diversity and relatedness between various banana cultivars is important to continuously improve its fruit characteristics, nutritional value, and disease resistance. This study aimed to develop a DNA fingerprinting panel using polymorphic simple sequence repeat (SSR) markers for the genetic characterisation of 11 banana cultivars in Malaysia. A total of 10 polymorphic SSR markers were developed into a multiplex PCR reaction and capillary electrophoresis to uniquely profile our collection of banana cultivars. The developed fingerprinting panel successfully amplified a total of 95 alleles, with 6 to 13 alleles per SSR marker. The average SSR marker polymorphic information content (PIC) value is 0.812, indicating the informativeness of the panel. Analysis of molecular variance (AMOVA) shows that 97% of the molecular variance from our banana collection is due to inter-variety genetic diversity, while the remaining 3% is due to intra-variety genetic diversity. A population structure analysis groups our collection of banana varieties according to the presence of at least one M. balbisiana (B) genome in their genetic makeup. The Cavendish variety, however, showed a distinct structure compared to the other cultivars. This SSR fingerprinting panel provides valuable insights into the genetic diversity and relatedness between banana cultivars in Malaysia. It has the potential to assist future banana breeding initiatives and serve as an effective quality control measure for verifying varieties in a tissue culture facility involved in banana planting materials production. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24242/1/ST%2010.pdf Sharmilah Vetaryan, and Hooi, Wei Yeng and Lee, Yang Ping (2024) Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers. Sains Malaysiana, 53 (7). pp. 1605-1616. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num7_2024/contentsVol53num7_2024.html |
| spellingShingle | Sharmilah Vetaryan, Hooi, Wei Yeng Lee, Yang Ping Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers |
| title | Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers |
| title_full | Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers |
| title_fullStr | Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers |
| title_full_unstemmed | Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers |
| title_short | Assessment of genetic diversity and relatedness of local banana (Musa spp.) cultivars in Malaysia using simple sequence repeat (SSR) markers |
| title_sort | assessment of genetic diversity and relatedness of local banana (musa spp.) cultivars in malaysia using simple sequence repeat (ssr) markers |
| url | http://journalarticle.ukm.my/24242/ http://journalarticle.ukm.my/24242/ http://journalarticle.ukm.my/24242/1/ST%2010.pdf |