Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS
The plant of Metroxylon sago Rottb. which is found abundantly in Indonesia, Malaysia, Philippines, and Papua New Guinea, is famous as carbohydrate producing plants. The sago fruit has not been yet extensively used, although they are rich in oligosaccharides which is potential as prebiotic. Sago frui...
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24118/ http://journalarticle.ukm.my/24118/1/SME%204.pdf |
| _version_ | 1848816017107582976 |
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| author | Fadhilla, Reza Yuliana, Nancy Dewi Kusnandar, Feri Kusumaningrum, Harsi Dewantari |
| author_facet | Fadhilla, Reza Yuliana, Nancy Dewi Kusnandar, Feri Kusumaningrum, Harsi Dewantari |
| author_sort | Fadhilla, Reza |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | The plant of Metroxylon sago Rottb. which is found abundantly in Indonesia, Malaysia, Philippines, and Papua New Guinea, is famous as carbohydrate producing plants. The sago fruit has not been yet extensively used, although they are rich in oligosaccharides which is potential as prebiotic. Sago fruit flour was studied as growth substrate for five selected probiotics and phytochemical profile of its methanol extracts were determined. All bacteria grew well up to 24 h on the growth medium containing sago fruit flour as carbohydrate substitute. However, Lacticaseibacillus rhamnosus FNCC 0099 and Lactobacillus acidophilus FNCC 0051 showed viability stability up to 72 h, while the others were slightly decreased. The sago fruit flour contained 20.4 mg/g total sugars, 14.8 mg QE/g flavonoids, and 36.7 mg GAE/g phenolics. After extraction of the fruit flour with 80% methanol using an ultrasonicator at 55 °C at 40 kHz for 40 min, the extract was analysed for its phytochemical profiles using untargeted LC-MS/MS screening with negative ionization mode. Seven compounds categorised in three distinct groups i.e., sugar alcohol, plant glycosides and fatty acids, have been identified as having possible prebiotic activity. These include ((1xi)-1,5-anhydro-2,3,6-tris-O-(carboxymethyl)-1-methyl-4-Omethyl-D-glucitol), quercitrin, (15Z)-9,12,13-trihydroxy-15-octadecenoic acid, corchorifatty acid F, 3,5-dihydroxy-2- (4-hydroxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl-hexopyranoside, gynocardin, and (4-methylumbelliferone)-βD-glucopyranoside. As the sago fruit flour has been proved as potential prebiotic in this study, its extensive prebiotics activity, prebiotic index, and stability under digestive condition will be subjected for further study. |
| first_indexed | 2025-11-15T00:59:11Z |
| format | Article |
| id | oai:generic.eprints.org:24118 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:59:11Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:241182024-09-05T03:41:11Z http://journalarticle.ukm.my/24118/ Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS Fadhilla, Reza Yuliana, Nancy Dewi Kusnandar, Feri Kusumaningrum, Harsi Dewantari The plant of Metroxylon sago Rottb. which is found abundantly in Indonesia, Malaysia, Philippines, and Papua New Guinea, is famous as carbohydrate producing plants. The sago fruit has not been yet extensively used, although they are rich in oligosaccharides which is potential as prebiotic. Sago fruit flour was studied as growth substrate for five selected probiotics and phytochemical profile of its methanol extracts were determined. All bacteria grew well up to 24 h on the growth medium containing sago fruit flour as carbohydrate substitute. However, Lacticaseibacillus rhamnosus FNCC 0099 and Lactobacillus acidophilus FNCC 0051 showed viability stability up to 72 h, while the others were slightly decreased. The sago fruit flour contained 20.4 mg/g total sugars, 14.8 mg QE/g flavonoids, and 36.7 mg GAE/g phenolics. After extraction of the fruit flour with 80% methanol using an ultrasonicator at 55 °C at 40 kHz for 40 min, the extract was analysed for its phytochemical profiles using untargeted LC-MS/MS screening with negative ionization mode. Seven compounds categorised in three distinct groups i.e., sugar alcohol, plant glycosides and fatty acids, have been identified as having possible prebiotic activity. These include ((1xi)-1,5-anhydro-2,3,6-tris-O-(carboxymethyl)-1-methyl-4-Omethyl-D-glucitol), quercitrin, (15Z)-9,12,13-trihydroxy-15-octadecenoic acid, corchorifatty acid F, 3,5-dihydroxy-2- (4-hydroxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-7-yl-hexopyranoside, gynocardin, and (4-methylumbelliferone)-βD-glucopyranoside. As the sago fruit flour has been proved as potential prebiotic in this study, its extensive prebiotics activity, prebiotic index, and stability under digestive condition will be subjected for further study. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24118/1/SME%204.pdf Fadhilla, Reza and Yuliana, Nancy Dewi and Kusnandar, Feri and Kusumaningrum, Harsi Dewantari (2024) Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS. Sains Malaysiana, 53 (6). pp. 1269-1280. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html |
| spellingShingle | Fadhilla, Reza Yuliana, Nancy Dewi Kusnandar, Feri Kusumaningrum, Harsi Dewantari Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS |
| title | Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS |
| title_full | Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS |
| title_fullStr | Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS |
| title_full_unstemmed | Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS |
| title_short | Metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by LC-MS/MS |
| title_sort | metroxylon sagu rottb. fruit flour as potential prebiotics for selected probiotics and phytochemical profiling of its methanolic extract by lc-ms/ms |
| url | http://journalarticle.ukm.my/24118/ http://journalarticle.ukm.my/24118/ http://journalarticle.ukm.my/24118/1/SME%204.pdf |