Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells
Matrix metallopeptidase 3 (MMP3) is among the key players in breast cancer metastasis that contributes to the highest cancer-related deaths in females globally. Previously, in silico analyses had shown that several coding single nucleotide polymorphisms (SNPs) of MMP3 were predicted to alter the sec...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24116/ http://journalarticle.ukm.my/24116/1/SME%202.pdf |
| _version_ | 1848816016560226304 |
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| author | Shafinah Ahmad Suhaimi, Soon, Choy Chan Pei, Pei Chong Norazalina Saad, De, Ming Chau Rozita Rosli, |
| author_facet | Shafinah Ahmad Suhaimi, Soon, Choy Chan Pei, Pei Chong Norazalina Saad, De, Ming Chau Rozita Rosli, |
| author_sort | Shafinah Ahmad Suhaimi, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Matrix metallopeptidase 3 (MMP3) is among the key players in breast cancer metastasis that contributes to the highest cancer-related deaths in females globally. Previously, in silico analyses had shown that several coding single nucleotide polymorphisms (SNPs) of MMP3 were predicted to alter the secondary structures of MMP3 and subsequently reduce its mRNA stability. To validate the mentioned hypotheses, this study aimed to determine the effects of six coding SNPs of MMP3 on its mRNA stability, protein expression level as well as cell invasiveness in vitro. In this study, breast adenocarcinoma MCF7 cells were transfected with MMP3 wild type (MMP3-WT) and a variant containing SNPs (MMP3-Var). Following transfection, protein expression level, mRNA stability and enzyme activity of MMP3-WT and MMP3-Var were evaluated. Finally, the effect of MMP3 coding SNPs on cell invasiveness in breast cancer was determined. In this study, the mRNA stability, protein expression level and enzymatic activity of MMP3-Var were significantly reduced. Moreover, the presence of MMP3 coding SNPs led to attenuated invasiveness of transfected MCF7 cells. In conclusion, these findings may contribute to the current understanding of these coding SNPs with metastasis in breast cancer. |
| first_indexed | 2025-11-15T00:59:10Z |
| format | Article |
| id | oai:generic.eprints.org:24116 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:59:10Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
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| spelling | oai:generic.eprints.org:241162024-09-05T03:25:50Z http://journalarticle.ukm.my/24116/ Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells Shafinah Ahmad Suhaimi, Soon, Choy Chan Pei, Pei Chong Norazalina Saad, De, Ming Chau Rozita Rosli, Matrix metallopeptidase 3 (MMP3) is among the key players in breast cancer metastasis that contributes to the highest cancer-related deaths in females globally. Previously, in silico analyses had shown that several coding single nucleotide polymorphisms (SNPs) of MMP3 were predicted to alter the secondary structures of MMP3 and subsequently reduce its mRNA stability. To validate the mentioned hypotheses, this study aimed to determine the effects of six coding SNPs of MMP3 on its mRNA stability, protein expression level as well as cell invasiveness in vitro. In this study, breast adenocarcinoma MCF7 cells were transfected with MMP3 wild type (MMP3-WT) and a variant containing SNPs (MMP3-Var). Following transfection, protein expression level, mRNA stability and enzyme activity of MMP3-WT and MMP3-Var were evaluated. Finally, the effect of MMP3 coding SNPs on cell invasiveness in breast cancer was determined. In this study, the mRNA stability, protein expression level and enzymatic activity of MMP3-Var were significantly reduced. Moreover, the presence of MMP3 coding SNPs led to attenuated invasiveness of transfected MCF7 cells. In conclusion, these findings may contribute to the current understanding of these coding SNPs with metastasis in breast cancer. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24116/1/SME%202.pdf Shafinah Ahmad Suhaimi, and Soon, Choy Chan and Pei, Pei Chong and Norazalina Saad, and De, Ming Chau and Rozita Rosli, (2024) Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells. Sains Malaysiana, 53 (6). pp. 1243-1253. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num6_2024/contentsVol53num6_2024.html |
| spellingShingle | Shafinah Ahmad Suhaimi, Soon, Choy Chan Pei, Pei Chong Norazalina Saad, De, Ming Chau Rozita Rosli, Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title | Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title_full | Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title_fullStr | Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title_full_unstemmed | Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title_short | Matrix metallopeptidase 3 coding SNPs suppress cell invasion in MCF7 breast cancer cells |
| title_sort | matrix metallopeptidase 3 coding snps suppress cell invasion in mcf7 breast cancer cells |
| url | http://journalarticle.ukm.my/24116/ http://journalarticle.ukm.my/24116/ http://journalarticle.ukm.my/24116/1/SME%202.pdf |