Exploring the resistance of pigmented rice to brown planthopper (Nilaparvata lugens Stål) by phenotypic and genotypic analyses
Developing new pigmented rice varieties that meet consumers’ preferences requires addressing resistance to brown planthopper (BPH), a highly destructive insect pest of rice. To effectively manage this pest, it is essential to explore BPH resistance genes from rice germplasms and incorporate them int...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24034/ http://journalarticle.ukm.my/24034/1/SEE%208.pdf |
| _version_ | 1848815997161570304 |
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| author | Chaerani, Yunus, Muhamad Hidayatun, Nurul Tasliah, Galurina, Rina Siti Matsohan, Andarini, Yusi Nurmalita Risliawati, Andari |
| author_facet | Chaerani, Yunus, Muhamad Hidayatun, Nurul Tasliah, Galurina, Rina Siti Matsohan, Andarini, Yusi Nurmalita Risliawati, Andari |
| author_sort | Chaerani, |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Developing new pigmented rice varieties that meet consumers’ preferences requires addressing resistance to brown planthopper (BPH), a highly destructive insect pest of rice. To effectively manage this pest, it is essential to explore BPH resistance genes from rice germplasms and incorporate them into elite varieties. The objective of this study was to identify the source of resistance among local and improved pigmented rice varieties in Indonesia against field BPH. Furthermore, the study aimed to characterize their genetic diversity using molecular markers associated with Bph genes and functional markers for these genes. Eighty-eight local varieties from the Indonesian Agricultural Gene Bank, collected across 18 provinces between 1971 and 2019, and four improved varieties, were evaluated using the standard seed box screening test method. Among these entries, 6 local accessions and 1 improved variety exhibited moderate resistance. Genetic analysis of these entries and three randomly selected susceptible entries using 12 SSR and InDel markers associated with Bph gene loci showed that the moderately resistant entries had higher allele numbers (11 to 13) compared to the susceptible entries (4 to 7). These markers could distinguish the resistant group from the susceptible ones in cluster analysis. Genotyping using functional markers further showed that the moderately resistant entries had 2 to 4 combinations of Bph genes. In contrast, the susceptible entries contained neither Bph genes nor only one gene. Local accessions with a 2 to 4 Bph gene combination can be donors of BPH resistance in improving pigmented rice varieties. |
| first_indexed | 2025-11-15T00:58:52Z |
| format | Article |
| id | oai:generic.eprints.org:24034 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:58:52Z |
| publishDate | 2024 |
| publisher | Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:240342024-08-19T03:12:58Z http://journalarticle.ukm.my/24034/ Exploring the resistance of pigmented rice to brown planthopper (Nilaparvata lugens Stål) by phenotypic and genotypic analyses Chaerani, Yunus, Muhamad Hidayatun, Nurul Tasliah, Galurina, Rina Siti Matsohan, Andarini, Yusi Nurmalita Risliawati, Andari Developing new pigmented rice varieties that meet consumers’ preferences requires addressing resistance to brown planthopper (BPH), a highly destructive insect pest of rice. To effectively manage this pest, it is essential to explore BPH resistance genes from rice germplasms and incorporate them into elite varieties. The objective of this study was to identify the source of resistance among local and improved pigmented rice varieties in Indonesia against field BPH. Furthermore, the study aimed to characterize their genetic diversity using molecular markers associated with Bph genes and functional markers for these genes. Eighty-eight local varieties from the Indonesian Agricultural Gene Bank, collected across 18 provinces between 1971 and 2019, and four improved varieties, were evaluated using the standard seed box screening test method. Among these entries, 6 local accessions and 1 improved variety exhibited moderate resistance. Genetic analysis of these entries and three randomly selected susceptible entries using 12 SSR and InDel markers associated with Bph gene loci showed that the moderately resistant entries had higher allele numbers (11 to 13) compared to the susceptible entries (4 to 7). These markers could distinguish the resistant group from the susceptible ones in cluster analysis. Genotyping using functional markers further showed that the moderately resistant entries had 2 to 4 combinations of Bph genes. In contrast, the susceptible entries contained neither Bph genes nor only one gene. Local accessions with a 2 to 4 Bph gene combination can be donors of BPH resistance in improving pigmented rice varieties. Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24034/1/SEE%208.pdf Chaerani, and Yunus, Muhamad and Hidayatun, Nurul and Tasliah, and Galurina, Rina Siti and Matsohan, and Andarini, Yusi Nurmalita and Risliawati, Andari (2024) Exploring the resistance of pigmented rice to brown planthopper (Nilaparvata lugens Stål) by phenotypic and genotypic analyses. Sains Malaysiana, 53 (5). pp. 1067-1080. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num5_2024/contentsVol53num5_2024.html |
| spellingShingle | Chaerani, Yunus, Muhamad Hidayatun, Nurul Tasliah, Galurina, Rina Siti Matsohan, Andarini, Yusi Nurmalita Risliawati, Andari Exploring the resistance of pigmented rice to brown planthopper (Nilaparvata lugens Stål) by phenotypic and genotypic analyses |
| title | Exploring the resistance of pigmented rice to brown planthopper (Nilaparvata lugens Stål) by phenotypic and genotypic analyses |
| title_full | Exploring the resistance of pigmented rice to brown planthopper (Nilaparvata lugens Stål) by phenotypic and genotypic analyses |
| title_fullStr | Exploring the resistance of pigmented rice to brown planthopper (Nilaparvata lugens Stål) by phenotypic and genotypic analyses |
| title_full_unstemmed | Exploring the resistance of pigmented rice to brown planthopper (Nilaparvata lugens Stål) by phenotypic and genotypic analyses |
| title_short | Exploring the resistance of pigmented rice to brown planthopper (Nilaparvata lugens Stål) by phenotypic and genotypic analyses |
| title_sort | exploring the resistance of pigmented rice to brown planthopper (nilaparvata lugens stål) by phenotypic and genotypic analyses |
| url | http://journalarticle.ukm.my/24034/ http://journalarticle.ukm.my/24034/ http://journalarticle.ukm.my/24034/1/SEE%208.pdf |