Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms
Pseudomonas aeruginosa is a pathogenic bacterium that can be considered a high risk to human health due to its remarkable capacity to resist antibiotics, either intrinsically or following the acquisition of resistance genes. P. aeruginosa has been considered a major threat to human health as all the...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2024
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| Online Access: | http://journalarticle.ukm.my/24032/ http://journalarticle.ukm.my/24032/1/SEE%206.pdf |
| _version_ | 1848815996582756352 |
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| author | Aqeel, Moaz Bukhari, Sayed Muhammad Ata Ullah Shah Ullah, Hidayat Hussain, Tahir Ullah, Aman Jamal, Muhsin Nawaz, Muhammad Asif Andleeb, Saadia Khan, Khurshaid Pervez, Sidra Imran, Muhammad |
| author_facet | Aqeel, Moaz Bukhari, Sayed Muhammad Ata Ullah Shah Ullah, Hidayat Hussain, Tahir Ullah, Aman Jamal, Muhsin Nawaz, Muhammad Asif Andleeb, Saadia Khan, Khurshaid Pervez, Sidra Imran, Muhammad |
| author_sort | Aqeel, Moaz |
| building | UKM Institutional Repository |
| collection | Online Access |
| description | Pseudomonas aeruginosa is a pathogenic bacterium that can be considered a high risk to human health due to its remarkable capacity to resist antibiotics, either intrinsically or following the acquisition of resistance genes. P. aeruginosa has been considered a major threat to human health as all the known remedies seem ineffective. Bacteriophages as a natural killer of bacteria can offer alternative therapy for antibiotics. The study aimed to isolate and characterize a lytic bacteriophage against P. aeruginosa (clinically resistant strain/superbug) and to investigate its lytic potential to control bacterial planktonic cells and their biofilms on stainless steel surfaces. A lytic bacteriophage known as MAC-1 was isolated from wastewater against the selected P. aeruginosa-2750 strain. Its host range was moderate and it only infected six isolates. Phage MAC-1 was characterized through transmission electron microscopy and was classified to the Siphoviridae family. It has good heat and pH stability. Its latent time was 24 min with a burst size of about 410 virions per cell. The phage MAC-1 efficacy was determined against P. aeruginosa planktonic cells and biofilms on metallic surfaces. Isolated phage MAC-1 demonstrated promising activity against bacterial planktonic cells as well as in reducing bacterial biofilm biomass formed in 96-well and on stainless steel plates. However, a phage cocktail may be used to avoid resistance and ensure complete eradication of bacterial biofilms. |
| first_indexed | 2025-11-15T00:58:51Z |
| format | Article |
| id | oai:generic.eprints.org:24032 |
| institution | Universiti Kebangasaan Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-15T00:58:51Z |
| publishDate | 2024 |
| publisher | Penerbit Universiti Kebangsaan Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | oai:generic.eprints.org:240322024-08-19T02:57:50Z http://journalarticle.ukm.my/24032/ Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms Aqeel, Moaz Bukhari, Sayed Muhammad Ata Ullah Shah Ullah, Hidayat Hussain, Tahir Ullah, Aman Jamal, Muhsin Nawaz, Muhammad Asif Andleeb, Saadia Khan, Khurshaid Pervez, Sidra Imran, Muhammad Pseudomonas aeruginosa is a pathogenic bacterium that can be considered a high risk to human health due to its remarkable capacity to resist antibiotics, either intrinsically or following the acquisition of resistance genes. P. aeruginosa has been considered a major threat to human health as all the known remedies seem ineffective. Bacteriophages as a natural killer of bacteria can offer alternative therapy for antibiotics. The study aimed to isolate and characterize a lytic bacteriophage against P. aeruginosa (clinically resistant strain/superbug) and to investigate its lytic potential to control bacterial planktonic cells and their biofilms on stainless steel surfaces. A lytic bacteriophage known as MAC-1 was isolated from wastewater against the selected P. aeruginosa-2750 strain. Its host range was moderate and it only infected six isolates. Phage MAC-1 was characterized through transmission electron microscopy and was classified to the Siphoviridae family. It has good heat and pH stability. Its latent time was 24 min with a burst size of about 410 virions per cell. The phage MAC-1 efficacy was determined against P. aeruginosa planktonic cells and biofilms on metallic surfaces. Isolated phage MAC-1 demonstrated promising activity against bacterial planktonic cells as well as in reducing bacterial biofilm biomass formed in 96-well and on stainless steel plates. However, a phage cocktail may be used to avoid resistance and ensure complete eradication of bacterial biofilms. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24032/1/SEE%206.pdf Aqeel, Moaz and Bukhari, Sayed Muhammad Ata Ullah Shah and Ullah, Hidayat and Hussain, Tahir and Ullah, Aman and Jamal, Muhsin and Nawaz, Muhammad Asif and Andleeb, Saadia and Khan, Khurshaid and Pervez, Sidra and Imran, Muhammad (2024) Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms. Sains Malaysiana, 53 (5). pp. 1043-1054. ISSN 0126-6039 https://www.ukm.my/jsm/english_journals/vol53num5_2024/contentsVol53num5_2024.html |
| spellingShingle | Aqeel, Moaz Bukhari, Sayed Muhammad Ata Ullah Shah Ullah, Hidayat Hussain, Tahir Ullah, Aman Jamal, Muhsin Nawaz, Muhammad Asif Andleeb, Saadia Khan, Khurshaid Pervez, Sidra Imran, Muhammad Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms |
| title | Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms |
| title_full | Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms |
| title_fullStr | Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms |
| title_full_unstemmed | Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms |
| title_short | Evaluation of the bacteriophage MAC-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms |
| title_sort | evaluation of the bacteriophage mac-1 potential to control pseudomonas aeruginosa planktonic cells and biofilms |
| url | http://journalarticle.ukm.my/24032/ http://journalarticle.ukm.my/24032/ http://journalarticle.ukm.my/24032/1/SEE%206.pdf |